Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bioactive polypeptide LPLP, and preparation and application thereof

A technology of biologically active polypeptides and uses, applied in the field of proteins, can solve problems such as human risks, and achieve the effect of promoting the body's immune activity, reducing damage, and enhancing phagocytic function.

Active Publication Date: 2015-06-24
ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Some early synthetic antioxidants such as butyl hydroxyanisole (BHA) and 2,6-di-tert-butyl-4-methylphenol (BHT) were used in food as lipid antioxidants, but these artificial Synthetic additives are potentially dangerous to the human body

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bioactive polypeptide LPLP, and preparation and application thereof
  • Bioactive polypeptide LPLP, and preparation and application thereof
  • Bioactive polypeptide LPLP, and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The preparation of embodiment 1 active peptide

[0051] 1. Preparation of fermented milk

[0052] 1) Lactobacillus helveticus fermented milk

[0053] Use skimmed milk powder (New Zealand NZMP brand skimmed milk powder) and water to prepare 12wt% skimmed milk (12g skimmed milk powder is added to 88g water, the same below). Under aseptic conditions, the tricyclic colony of Lactobacillus helveticus (CICC6024) was picked, added to sterilized 12wt% skim milk, and stirred evenly under aseptic conditions. After inoculation, seal with aluminum foil to prevent contamination. Place in an incubator at 37°C for 19 hours. After the cultivation is over, the curd is stirred evenly under aseptic conditions to complete the activation of the Lactobacillus helveticus and prepare the starter for preparing the Lactobacillus helveticus fermented milk.

[0054] Inoculate 10mL of the prepared Lactobacillus helveticus starter into 500mL of sterilized 12wt% skim milk (inoculation rate 2v / v%)...

Embodiment 2

[0126] Antioxidant activity experiment of embodiment 2 bioactive polypeptides

[0127] The antioxidant activity of the biologically active polypeptide LPLP obtained in Example 1 was tested by free radical scavenging method (DPPH·method) and total antioxidant capacity method (Ferric Reducing Ability Power FRAP method).

[0128] 1. Determination of in vitro antioxidant activity of biologically active polypeptide LPLP by [DPPH·] method

[0129] 1) Experimental reagents and instruments

[0130] Reagent: 1,1-diphenyl-2-trinitrophenylhydrazine (1,1-Diphenyl-2-picrylhydrazyl[DPPH·]), produced by Wako Company of Japan; Methanol, provided by Shanghai Sinopharm Company; obtained in Example 1 Milk-derived bioactive polypeptide LPLP obtained by fermentation of Lactobacillus helveticus (collected peak B sample).

[0131] Main instruments: Sunrise microplate reader, product of Tecan Company of Austria; 96-well cell culture plate, manufactured by Millipore Company of the United States; ana...

Embodiment 3

[0168] Example 3 Bioactive Polypeptide LPLP Immunity-Promoting Activity Experiment

[0169] 1. MTT method to determine the in vitro macrophage proliferation ability of biologically active polypeptide LPLP

[0170] 1) Experimental reagents and instruments

[0171] Reagent: experimental animal balb / c mouse (male 6-8 weeks old) Animal Experiment Center, College of Agriculture and Biology, Shanghai Jiaotong University; milk-derived bioactive polypeptide LPLP fermented by Lactobacillus helveticus; 3-(4,5-di Methylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT) Amresco; LPS (lipopolysaccharide), purchased from Sigma; bovine serum albumin (Bovine Serum Albumin, BSA) Genebase; Triple solution, containing 10% SDS, 5% isobutanol and 0.012mol / L HCl in water.

[0172] Equipment: LRH-250F biochemical incubator Shanghai Heng Technology Co., Ltd.; GL-22M high-speed refrigerated centrifuge Shanghai Luxiangyi Centrifuge Instrument Co., Ltd.; Hera cell 150CO 2 Incubator Heraeus; Dragon Wel...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
collision energyaaaaaaaaaa
correlation coefficientaaaaaaaaaa
Login to View More

Abstract

Provided is a milk-derived biologically active polypeptide having an amino acid sequence of LPLP, which has in-vitro antioxidation activity and enhances immunity of human body. The polypeptide has good biological antioxidation activity, and has the function of enhancing immunity of cells and human body.

Description

technical field [0001] The invention relates to the field of proteins, in particular to a bioactive polypeptide LPLP and its preparation and application. Background technique [0002] During the fermentation process of milk by lactic acid bacteria, a part of protein in milk is metabolized and utilized by lactic acid bacteria, and a series of physiological and biochemical reactions occur, making the protein into polypeptides or free amino acids, which are digested and absorbed by the human body or absorbed and transported by small intestinal epithelial cells directly into the blood circulation of the human body. Among these polypeptides, some have special physiological functions and are called "biologically active polypeptides". [0003] Oxidation reaction and oxidative metabolism are crucial for food and human body, and free radicals and reactive oxygen species cause a series of oxidation reactions. When excessive free radicals are formed, they will exceed the protective e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/103C12N15/12C12P21/06C07K1/36C07K1/34C07K1/20A23L1/305A61K38/07A61P39/06A61P37/04C12R1/225
CPCA23L33/18A61K38/018A61K38/07A61P37/04A61P39/06C07K5/101C12P21/02C12P21/06
Inventor 张少辉卢姗姗孙冠华马鎏镠周婕慧李锡安占东升
Owner ZHEJIANG HUITAI LIFE HEALTH TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com