Method for preparing fermentable sugar from water-washed alkaline pretreated plant fiber raw material

A plant fiber and fermentable sugar technology, which is applied in the field of fermentable sugar preparation, can solve the problems of large water consumption, large amount of waste water, high production cost, etc., and achieve the effects of ensuring recovery, ensuring high efficiency, and reducing inhibition

Active Publication Date: 2013-11-27
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional pulping and papermaking process uses a large amount of water to wash the alkaline pretreated plant fiber raw materials to achieve the purpose of removing residual chemicals, which consumes a lot of water, produces a large amount of wastewater, and has high production costs.

Method used

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  • Method for preparing fermentable sugar from water-washed alkaline pretreated plant fiber raw material
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  • Method for preparing fermentable sugar from water-washed alkaline pretreated plant fiber raw material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 The washing of green liquid pretreatment corn stalk

[0021] Corn stalks are crushed to 2-5cm, and the green liquor (Na 2 CO 3 and Na 2 S) is the pretreatment cooking liquid, the amount of alkali used is 8% (w / w), the degree of sulfidation is 40%, the ratio of cooking solid to liquid is 1:6 (w / v), after idling at 60°C for 0.5h, the temperature Raise it to 140°C, keep it warm for 1 hour, and after cooling, refine and filter out most of the black liquor together with the black liquor to recover chemicals. The filtered corn stalks are neutralized with acid and washed in three batches with solid-to-liquid ratios of 1:5, 1:10, and 1:15: add 1 / 3 of the washing water to the first stage of washing, and add a certain amount neutralize the acid to pH 4.5-5.5, and filter; add 1 / 3 of the washing water to the second and third sections respectively, and filter and squeeze the material dry. Collect the black liquor and the filtrate of the third stage separately, and a...

Embodiment 2

[0026] Example 2 Cellulase hydrolysis of washed corn stalks at a substrate concentration of 5% (w / v)

[0027]Take by weighing respectively the water-washed corn stalks of Example 1 whose absolute dry weight is 2.50g in three 250mL conical flasks, add 1mol / L citric acid buffer solution 2.5mL in each conical flask, and the cellulase consumption is 13.94 FPIU / g green liquid pretreatment corn stalk (25FPIU / g dextran, cellulase activity: β-glucosidase activity: xylanase activity=1:1.15:6.61) in each Erlenmeyer flask, add corresponding For enzyme, add an appropriate amount of distilled water to each Erlenmeyer flask so that the total volume of water in the enzymolysis system is 50mL, mix the reaction system thoroughly with a glass rod, cover it, and place it in a constant temperature shaker at 150 rpm and 50°C. Enzymolysis for 48h. After the hydrolysis, the hydrolyzate was centrifuged at 4000 rpm for 10 min, and the supernatant was taken to measure the concentration of glucose and...

Embodiment 3

[0031] Example 3 Concentration and ethanol fermentation of hydrolyzed sugar solution

[0032] Weigh the water-washed corn stalks of Example 1 with a dry weight of 25 g at a substrate concentration of 5% (w / v), and the amount of cellulase is 13.94 FPIU / g water-washed corn stalks (25 FPIU / g dextran, cellulose Enzyme activity: β-glucosidase activity: xylanase activity=1:1.15:6.61), carry out enzymatic hydrolysis under the condition of pH4.8-5.0, cover the cover after fully mixing the reaction system with glass rod, at 150 RPM, 50°C constant temperature shaker for 48h. After solid-liquid separation, the supernatant was concentrated on a rotary evaporator at 70°C and 160mbar to a glucose concentration of 150-200g / L.

[0033] Take 100mL of the above-mentioned concentrated sugar solution in a 250mL conical flask, add 0.024g urea, 0.008g magnesium sulfate, 0.008g zinc sulfate, 1.0g active Saccharomyces cerevisiae dry powder, and cover with a rubber stopper (insert a needle on the rub...

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Abstract

The invention discloses a method for preparing fermentable sugar from a water-washed alkaline pretreated plant fiber raw material, which comprises the steps of raw material pretreatment, washing and enzyme hydrolysis, wherein in the washing step, after filtering the pretreated plant fiber raw material, neutralizing with acid, washing a small amount of water to obtain a substrate susceptible to enzyme hydrolysis, and carrying out enzyme hydrolysis to prepare the fermentable sugar. According to the method, the plant fiber raw material is pretreated by green liquor and filtered, neutralized by acid and subjected to batch section washing according to a solid-to-liquid ratio of 1:(2-20), and the substrate with the concentration (w / v) of 5-15% is subjected to cellulase batch hydrolysis for 48-72 hours to obtain the fermentable sugar. The method recovers chemicals and heat energy by using the existing mature technique in pulping paper technology, reduces the consumption of washing water and the generation of wastewater, lowers the environmental pollution, and implements the clean production of fermentable sugar.

Description

technical field [0001] The invention relates to a preparation process of fermentable sugar, in particular to a method for preparing fermentable sugar by washing alkaline pretreated plant fiber raw materials. Background technique [0002] With the exploitation and utilization of fossil resources represented by petroleum in human society and the global warming trend caused by the extensive use of fossil resources, it has become a research hotspot in recent years to find renewable resources to replace fossil raw materials to produce energy and chemicals. Biomass resources have become one of the best raw materials for petroleum substitution strategies due to their rich content, renewable characteristics, and carbon dioxide absorption during the growth process. Biomass resources that can be used to produce energy and chemicals mainly include starchy raw materials, sugar raw materials and plant fiber raw materials. Due to the constraints of the contradiction between global food s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02
CPCY02P20/59
Inventor 勇强储秋露余世袁李鑫朱均均徐勇马斌杨德良
Owner NANJING FORESTRY UNIV
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