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Preparation and application of a biologically functionalized hydroxypropyl cellulose ester liquid crystal film

A hydroxypropyl cellulose, hydroxypropylated technology, applied in liquid crystal materials, chemical instruments and methods, medical science and other directions, to achieve the effect of improving cell affinity, improving bioavailability, and improving utilization

Active Publication Date: 2015-12-09
赣州铂延生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

RGD peptide chains have been used to combine with a variety of different biomaterials such as hyaluronic acid and polyethylene glycol hydrogels, titanium alloy implants, polyurethane and polydimethylsilane surfaces, and implemented on the surface of liquid crystal biomaterials. RGD functionalization remains unreported

Method used

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  • Preparation and application of a biologically functionalized hydroxypropyl cellulose ester liquid crystal film
  • Preparation and application of a biologically functionalized hydroxypropyl cellulose ester liquid crystal film
  • Preparation and application of a biologically functionalized hydroxypropyl cellulose ester liquid crystal film

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1: Synthesis of hydroxypropyl cellulose propyl ester-glutaric anhydride liquid crystal and the preparation process of its surface grafted RGD-containing short peptide:

[0050] (1) 3g hydroxypropyl cellulose was reacted with 1.2mL propionyl chloride in acetone at 55°C for 6 hours with magnetic stirring, then dialyzed in water for 3 days, changing the water 3 times a day, and vacuum-dried at 50°C to obtain low Substituted for hydroxypropyl cellulose propyl ester (PPC).

[0051] (2) Dissolve 3g of low-substituted PPC in 50mL of distilled acetone and heat to 50°C, add 2g of glutaric anhydride (GA) and 0.15g of 4-lutidine to the homogeneous solution, and react for 45 minutes under magnetic stirring. The reaction product was transferred to a dialysis bag, dialyzed in ultrapure water for 3 days, and the water was changed 3 times a day. After the dialysis, the product was dried in a vacuum oven at 50°C, and the obtained product was designated as PPC-GA.

[0052] (3) P...

Embodiment 2

[0055] Example 2: Synthesis of hydroxypropyl cellulose butyl ester-glutaric anhydride liquid crystal and the preparation steps of its surface grafted with RGDS short peptide:

[0056] (1) 3g of hydroxypropyl cellulose was reacted with 1mL of butyryl chloride in acetone, 60°C, magnetically stirred for 5 hours, dialyzed in water, and dried in vacuum at 55°C to obtain low-substituted hydroxypropyl cellulose butyl ester (BPC ).

[0057] (2) Dissolve 3g of low-substituted BPC in 50mL of distilled acetone and heat to 50°C, add 2g of glutaric anhydride (GA) and 0.15g of 4-lutidine to the homogeneous solution, and react for 45 minutes under magnetic stirring. The reaction product was transferred to a dialysis bag, dialyzed in ultrapure water for 5 days, and the water was changed 3 times a day. After the dialysis, the product was dried in a vacuum oven at 50°C, and the obtained product was designated as BPC-GA.

[0058] (3) BPC-GA was dissolved in tetrahydrofuran to form a homogeneous...

Embodiment 3

[0061] Embodiment 3: the preparation of hydroxypropyl cellulose octyl ester (OPC)-glutaric anhydride film surface grafting containing RGD short peptide, comprises the following steps:

[0062] (1) 3 g of hydroxypropyl cellulose was reacted with 1 mL of octanoyl chloride in acetone, 60 ° C, magnetically stirred for 4 hours, dialyzed in water, and dried in vacuum at 55 ° C to obtain low-substituted hydroxypropyl cellulose octyl ester (OPC ).

[0063] (2) Dissolve 5g of low-substituted OPC in 50mL of distilled acetone and heat to 50°C, add 3g of glutaric anhydride and 0.225g of 4-lutidine to the homogeneous solution, and react for 1 hour under magnetic stirring. After the reaction, the reaction product was transferred to a dialysis bag and dialyzed in ultrapure water for 5 days, changing the water 3 times a day to remove by-products and unreacted small molecules. After dialysis, the product was dried in a vacuum oven at 50°C to remove all moisture to obtain the product, which wa...

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Abstract

The invention discloses preparation and application for a bio-functional hydroxy propyl cellulose ester type liquid crystal membrane, and belongs to the field of biomedical materials. The preparation comprises the following steps: firstly, preparing a low-substituted alkyl acyl hydroxy propyl cellulose ester type liquid crystal by an acylating chlorination reaction of hydroxy propyl celluloses; reacting with glutaric anhydride and grafting a carboxyl functional group to the hydroxy propyl cellulose ester type liquid crystal; then preparing a liquid crystal membrane by adopting a solution pouring method; and activating the carboxyl on the surface of a thin membrane by EDC / NHS (carbodiimide / N-Hydroxysuccinimide) and carrying out a heterogeneous reaction with an oligopeptide containing RGDS (Arg-Gly-Asp-Ser) to obtain the bio-functional hydroxy propyl cellulose ester type liquid crystal with the surface which is grafted with the oligopeptide containing the RGDS. The liquid crystal thin membrane with the surface which is fixedly provided with the oligopeptide containing the RGDS has extracellular-matrix-like structure and component; the cell-material mutual effect is effectively improved; the adhering and the multiplication of cells on the surface of the material are promoted; the liquid crystal thin membrane can be used as a cell model or a cell bracket to be applied to the fields including tissue engineering, regenerative medicines and the like.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to the preparation and application of a biologically functionalized hydroxypropyl cellulose ester liquid crystal film. Background technique [0002] A fundamental theme in biomaterials and regenerative medicine is cell-material interactions, and ideal biomaterials should maximize beneficial responses to cells or tissues. Tissue engineering is an emerging technology for the repair and regeneration of damaged tissues by applying the principles of life science and engineering, with the help of biomaterials combined with cells and appropriate stimulating factors. One of the many strategies in tissue engineering technology is to design biomaterials that can provide initial support for cell adhesion and structural scaffolding, and provide a structural framework for cells to form functional tissues. In recent years, many methods have been developed to modify the surface of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/12C08J5/18C08B3/12C09K19/38A61L27/20
Inventor 屠美查振刚曾戎吴昊韩婉清
Owner 赣州铂延生物科技有限公司
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