Combined extraction preparation method of complex polypeptide

Abstract

The invention provides a combined extraction preparation method of complex polypeptide. According to the combined extraction preparation method, a moderate weak acid method is adopted for extracting natural bioactive polypeptide, then sediments remained and discarded in the polypeptide extraction process with the weak acid method are hydrolyzed by adopting an enzymolysis method, namely the same raw material is subjected to combined polypeptide extraction by respectively adopting the acid hydrolysis method and the enzymolysis method and utilizing the extraction method, extracted raw material or resource is completely utilized, original waste is used to the greates extent, and yield of extracted and prepared combined polypeptide is obviously improved. In addition, valuable natural resources are saved, environmental pollution is reduced, potential safety hazard is eliminated, production period is shortened, extraction reagents and instruments are reduced, investment and production cost are reduced, polypeptide bioavailability is improved, future use application scope is extended, and human body application value is improved.

Description

technical field [0001] The invention relates to applications in related fields such as medical beauty care and anti-aging products, in particular to a combined extraction and preparation method of a composite polypeptide. Background technique [0002] 1. Technical background of peptides and their applications [0003] Peptides are divided into oligopeptides and polypeptides, which are a type of biologically active substances that are condensed by two or more amino acids and linked by peptide bonds. It is an intermediate product of proteins, and its molecular structure is between amino acids and proteins. fragments in between. Compared with other substances in living organisms, peptide substances are characterized by their high biological activity and biodiversity. It is precisely because they have a wide range of biological activities, they are also called active biological peptides. Polypeptide is a part of peptides and one of the most basic and important substances that...

AI Technical Summary

Problems solved by technology

If the amount of hydrolytic enzyme used in the enzymatic hydrolysis method is too large or the pH condition is too severe, etc., it may also cause irreversible denaturation or excessive hydrolysis of the extract, as well as significant changes in molecular conformation, making it gradually lose the biological activity and biological activity of the polypeptide. function, so that the extracted polypeptide loses its due efficacy and application value

Because the most suitable pH value for maintaining acidic protease is 2.0-2.5, and the optimal pH value for alkaline protease is 11, the reaction conditions are relatively severe, which not only requires high equipment and is not easy to control the degree of hydrolysis. no practical significance

Although neutral protease opens the peptide bond from the inside of the protein, it has outstanding specificity, relatively mild reaction conditions (pH=7), moderate hydrolysis effect, easy to control operation, and will not destroy amino acid structure and other nutritional components. It has incomparable advantages in the retention of components, and is cheap, suitable for large-scale industrial production. However, if the amount of hydrolytic enzymes is too much or too little, the selection of enzymes is unreasonable, or the pH condition is too severe, the extraction yield is often On the low side, the gain outweighs the gain

Moreover, in order to prevent the added protease from continuing to hydrolyze after the polypeptide extraction is completed, it is usually necessary to boil to inactivate the enzyme, which may reduce or destroy the biological activity of the polypeptide to a certain extent.

[0009] However, if the acid-alcohol method is used to extract polypeptides, the pH value of the extraction liquid is usually 3.5 or below to have a better extraction yield and separation effect. However, although this pH value cannot seriously change its molecular structure and biological activity, this method cannot Extracted peptides are difficult to dissolve when the pH is higher than 3.5 or above and under general conditions, which not only affects its solubility, but also affects practical applications

If the pH of its dissolution system is reduced to 3.5 or below for the dissolution of the extracted polypeptide, it will be difficult to apply directly in some fields such as skin cosmetics and cosmetics

Moreover, the acid-alcohol method is used to extract polypeptides. The ethanol used not only increases the extraction cost, but also pollutes the environment and affects the health of the operator. Moreover, the separation and removal of ethanol remaining in the polypeptide extract and the subsequent recovery of ethanol are cumbersome. , more difficult

However, purely using ultrafiltration to extract and separate peptides is simple and suitable for large-scale production, but technical bottlenecks such as membrane plugging, actual service life and cost, and peptide extraction yield still need to be further improved and improved.

Moreover, the currently used polypeptide ultrasonic extraction methods, microwave extraction methods, etc. also have problems or deficiencies of one kind or another to varying degrees.

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