Method for rapid determination of aluminum ion content in honey product

An aluminum ion, fast technology, applied in the preparation of test samples, color/spectral property measurement, etc., can solve the problems of cumbersome method system, high measurement cost, long time consumption, etc., to achieve simple operation, reproducibility and precision. Good, the effect of measuring a wide range of concentrations

Active Publication Date: 2014-08-20
武威市顶乐生态牧业有限公司
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  • Abstract
  • Description
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Problems solved by technology

[0006] Among the existing detection methods, the spectrophotometric method using chromazurin as the chromogenic agent is the most widely used, and the research application is also the most extensive. However, the method system is cumbersome, and emulsifiers and sensitizers need to be added as sample pretreatment (solubilization) and One of the means of method sensitivity
Methods such as atomic absorption spectroscopy (AAS), inductively coupled plasma atomic emission spectrometry (ICP) and ICP mass spectrometry (ICP-MS) all require nitric acid, perchloric acid digestion, microwave digestion and ashing digestion to process samples. Long time-consuming, low efficiency, high measurement cost

Method used

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  • Method for rapid determination of aluminum ion content in honey product
  • Method for rapid determination of aluminum ion content in honey product
  • Method for rapid determination of aluminum ion content in honey product

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Embodiment 1: the preparation of early stage solution

[0046] 1. Preparation of reaction test solution: Preparation of Alizarin Red S solution (0.25g / L) Weigh 0.025g of Alizarin Red S, dissolve in 50mL of absolute ethanol, dilute with distilled water to 100mL.

[0047] 2. Preparation of aluminum ion standard solution (1.0mg / mL): weigh 0.89g AlCl 3 ·6H 2 O (aluminum content is 0.1g), dissolved in deionized water and adjusted to 100mL.

[0048] 3. Preparation of disodium hydrogen phosphate-citric acid buffer solution (pH4.6): Accurately pipette 46.75 mL of 0.2 mol / L disodium hydrogen phosphate solution and 53.25 mL of 0.1 mol / L citric acid solution, and mix to 100 mL.

Embodiment 2

[0049] Embodiment 2: Determination of reaction system and maximum absorption peak

[0050] The natural color of Alizarin Red S solution is yellow. In the acidic medium of disodium hydrogen phosphate-citric acid at pH 4.6, it forms a stable complex water-soluble substance with a certain concentration of aluminum ions. The color is red. Before and after the solution is scanned at full wavelength, it shows that the maximum light absorption peak of Alizarin Red S solution is at 420nm (such as figure 1 ), the maximum absorption peak of the solution is at 490nm after reacting with aluminum ions (such as figure 2 ). Therefore, the components of the reaction system were determined to be: pH4.6 disodium hydrogen phosphate-citric acid buffer solution, alizarin red S solution and aluminum ion solution, and the detection wavelength was 490nm.

Embodiment 3

[0051] Embodiment 3: Optimization of reaction system components and conditions

[0052] On the basis of Example 2, the reaction system and reaction conditions were optimized, and different additions of 0.25g / L Alizarin Red S solution (0.8mL, 1.0mL, 1.2mL, 1.5mL, 1.8mL and 2.0mL ), pH4.6 disodium hydrogen phosphate-citric acid buffer (0.1mL, 0.15mL, 0.20mL and 0.25mL,) and 1.0mg / mL aluminum ion standard solution addition (0.1mL, 0.2mL, 0.3mL , 0.4mL, 0.5mL, 0.6mL, 0.7mL, 0.8mL, 0.9mL and 1.0mL) on the sensitivity and accuracy of the detection method. As a result, the best reaction system is: the total volume of the reaction system is 5.0mL, which is not enough to be constant to volume with deionized water, and wherein the Alizarin Red S solution addition is 1.5mL (such as image 3 ), pH4.6 disodium hydrogen phosphate-citric acid buffer solution is added in an amount of 0.15mL (such as Figure 4 ) and the determination range of aluminum ion is 0.2-0.7mg / mL (such as Figure 5 ...

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Abstract

The invention relates to a method for rapid determination of aluminum content in a honey product; the method is an ultraviolet-visible spectrophotometry method mainly based on using alizarin red S as a complexing reagent; and the method can shorten the sample determination time, saves resources, is good in reproducibility and stability and high in recovery rate, and can meet the practical requirements of laboratory determination.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, in particular to a method for quickly measuring the aluminum ion content in honey products. Background technique [0002] Aluminum salts are widely used in tableware and food additives in the catering industry, water treatment agents in environmental governance, and immune adjuvants in medicine, etc., and have become an indispensable part of daily life. However, once excessive aluminum salt enters the human body through food, medicine, drinking water and other ways, it will show biological toxicity effects. Studies have shown that senile dementia (Alzheimer's disease), Guam Parkinson's dementia syndrome, amyotrophic lateral sclerosis, and dialysis encephalopathy and other neurological disorders, osteomalacia and microcytic anemia, etc. Related to the excessive intake of aluminum salts, the related diseases are called aluminum encephalopathy and aluminum bone disease. These studies...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/33G01N1/34
Inventor 王晓力王永刚朱新强王春梅张茜路远汪晓斌杨晓
Owner 武威市顶乐生态牧业有限公司
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