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Porous support for cartilage tissue engineering and preparation method thereof

A porous scaffold and cartilage tissue technology, applied in medical science, prosthesis, etc., can solve the problems of low porosity, solvent residue, and difficult to control pore size, so as to increase the strength and hardness of the gel and improve the stability of the gel structure , cross-linking and compounding sufficient effect

Active Publication Date: 2014-08-27
BEOGENE BIOTECH GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the traditional microsphere sintering method, the porosity is low; the solution casting / particle leaching method is easy to cause solvent residue, and the volatile solvent is not environmentally friendly; the thermally induced phase separation / freeze drying method is not accurate enough in controlling porosity and pore size; High-tech methods such as spinning method and supercritical fluid preparation method do have great prospects in the future, but their current cost is too high, and large-scale use is still unrealistic
[0005] The cross-linked complex of carrageenan-chitosan has been confirmed by many scholars, and some have also prepared carrageenan-chitosan pellets, but the cross-linked compound of carrageenan-chitosan as a three-dimensional porous scaffold is still There are many problems, such as the hardness of carrageenan is not strong, the porosity of the gel is not high, and the pore size is difficult to control, etc.

Method used

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  • Porous support for cartilage tissue engineering and preparation method thereof
  • Porous support for cartilage tissue engineering and preparation method thereof
  • Porous support for cartilage tissue engineering and preparation method thereof

Examples

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Embodiment 1

[0036] A porous scaffold for cartilage tissue engineering, which is a three-dimensional porous scaffold composed of κ-type carrageenan and chitosan with a molecular weight of 100,000 and a deacetylation degree of 90% in a mass ratio of 6:1, with a porosity of 89% and a pore size of 100- 150μm, compressive modulus 0.7MPa, tensile modulus 1.0MPa.

[0037] A method for preparing a porous scaffold for cartilage tissue engineering, comprising the following steps:

[0038] (1) Prepare 3% carrageenan solution with 60°C distilled water, prepare 0.6% chitosan solution with PH3 dilute hydrochloric acid solution, use beater 8000r / min respectively, beat for 30s;

[0039] (2) Blend the above-mentioned carrageenan and chitosan solution according to the carrageenan-chitosan solute mass ratio of 6:1, and stir with a magnetic stirrer at 1000 r / min to obtain a carrageenan-chitosan compound;

[0040] (3) beat the carrageenan-chitosan complex, add 120 μm sodium carbonate crystal powder (the mass...

Embodiment 2

[0044] A porous scaffold for cartilage tissue engineering, which is a three-dimensional porous scaffold composed of κ-type carrageenan and chitosan with a molecular weight of 200,000 and a degree of deacetylation of 85% in a mass ratio of 4:1, with a porosity of 92% and a pore size of 50 to 50%. 80μm, compressive modulus 1.2MPa, tensile modulus 2.2MPa.

[0045] A method for preparing a porous scaffold for cartilage tissue engineering, comprising the following steps:

[0046] (1) Prepare 5% carrageenan solution with 65°C distilled water, prepare 1% chitosan solution with PH3.6 dilute acetic acid solution, use beater 8500r / min respectively, beat for 25s;

[0047] (2) Blend the above carrageenan and chitosan solution according to the carrageenan and chitosan solute mass ratio of 5:1, and stir with a magnetic stirrer at 1500r / min to obtain a carrageenan-chitosan compound;

[0048] (3) beat the carrageenan-chitosan complex, add 100 μm sodium carbonate crystal powder (the mass rati...

Embodiment 3

[0052] A porous support for cartilage tissue engineering, which is a mixed glue of κ-type carrageenan, ι-type carrageenan and λ-type carrageenan (the mixing mass ratio is κ-type carrageenan: ι-type carrageenan: λ-type carrageenan=8: 1:1) and chitosan with a molecular weight of 330,000 and a deacetylation degree of 92% are a three-dimensional porous scaffold composed of a mass ratio of 8:1. Volume 2.0MPa.

[0053] A method for preparing a porous scaffold for cartilage tissue engineering, comprising the following steps:

[0054] (1) Prepare 10% carrageenan solution with 67°C distilled water, prepare 0.5% chitosan solution with PH3.3 dilute hydrochloric acid solution, use beater 10000r / min respectively, beat for 25s;

[0055] (2) blending the above-mentioned carrageenan and chitosan solution according to the carrageenan and chitosan solute mass ratio of 8:1, and stirring with a magnetic stirrer at 2000r / min to obtain a carrageenan-chitosan compound;

[0056] (3) beat the carrag...

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Abstract

The invention relates to a porous support for cartilage tissue engineering and a preparation method thereof, and belongs to the technical field of biological medical materials. The porous support is a three-dimensional porous support comprising carragheenan and chitosan and has porosity of 80-96% and aperture sizes of 50-500 microns. The preparation method comprises the following steps of preparing a carragheenan aqueous solution from distilled water, preparing a chitosan solution from a dilute acid solution, respectively beating the solutions, then blending the solutions, carrying out stirring, adding sodium carbonate crystal powder into the mixture, carrying out beating again, carrying out water-bath treatment, pouring the treated mixture into a stainless steel mold, carrying out freeze drying, carrying out immersion in a dilute acid solution, carrying out immersion in distilled water and carrying out vacuum drying to obtain the porous support for cartilage tissue engineering. The preparation method utilizes the raw material having wide sources and safety. The porous support has a certain function, high hardness, high porosity and no residue and is suitable for tissue cell culture or cartilage regeneration. The preparation method has simple and easy processes and a wide industrial prospect.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a porous scaffold for tissue engineering and a preparation method thereof. Background technique [0002] Carrageenan, also known as carrageenan, is a natural anionic polysaccharide plant colloid extracted from red algae. According to different structure types, it can be divided into κ-type carrageenan, ι-type carrageenan and λ-type carrageenan, etc., and κ-type carrageenan has the best coagulation property. Carrageenan is white to light yellowish brown, glossy on the surface, translucent sheet or powder, odorless and tasteless, sticky and slippery in taste, swells in cold water, can be dissolved in hot water above 60 ℃ to form viscous transparent or Slight milky white free flowing solution, insoluble in organic solvents. Carrageenan is easy to gel and has thermal reversibility. After heating and dissolving, it can form a semi-solid transparent gel when it is cooled...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/20A61L27/56
Inventor 郭瑞熊胜全
Owner BEOGENE BIOTECH GUANGZHOU
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