Preparation method of chitosan-silk fibroin composite nanofiber multifunctional patch for promoting myocardial tissue regeneration and stem cell monitoring

A composite nanofiber, myocardial tissue regeneration technology, applied in the direction of bone/connective tissue cells, animal cells, vertebrate cells, etc., can solve the problems that are not conducive to artificial improvement and regulation, and cannot achieve sustained release of drugs or cytokines. The effect of promoting attachment and growth, inhibiting left ventricular remodeling, and increasing specific surface area
CN104189958BActive Publication Date: 2015-12-02GENERAL HOSPITAL OF PLA +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GENERAL HOSPITAL OF PLA
Publication Date
2015-12-02

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Abstract

The invention relates to a method for preparing a chitosan-silk fibroin composite nano-fiber multifunctional patch for promoting myocardial tissue regeneration and monitoring stem cells. The method comprises the following steps: preparing a cellulose nano-fiber basal plate by an electrostatic spinning technology; alternately assembling positively charged chitosan (CS) and negatively charged silk fibroin (SF) to the surface of nano-fibers layer by layer by adopting a layer-by-layer assembly technique, and assembling 5.5-10.5 layers to form a CS-SF composite nano-fiber membrane; and planting seed cells of adipose tissue-derived stromal cells or cardiac progenitor cells labeled by green fluorescent protein and firefly luciferase on the surface of the CS-SF composite nano-fiber membrane, preparing the patch by three-dimensional co-culture. The patch has excellent biocompatibility, can be used as a cell vector, has an effect of resisting oxidative stress to improve the survival rate and treatment efficiency of stem cells, and is capable of evaluating the number, distribution and function states of transplanted stem cells, effectively preventing occurrence of post-myocardial infarction heart failure and reducing the death rate of ischemic cardiomyopathy.
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Description

technical field

[0001] The invention relates to a tissue engineering technology, in particular to a preparation method of a chitosan-silk fibroin composite nanofiber patch for promoting myocardial tissue regeneration and stem cell detection after acute myocardial infarction. Background technique

[0002] According to the statistics of the World Health Organization in 2012, ischemic heart disease has become the number one killer of human health worldwide, accounting for 13.2% of all-cause mortality, and about half of the patients died of acute myocardial infarction. The mammalian heart is a terminally differentiated organ. Once necrotic, it is difficult to regenerate and repair itself. With the necrosis and massive loss of cardiomyocytes, it will lead to the progress of ventricular remodeling and eventually heart failure. At present, traditional treatment methods, such as coronary artery bypass surgery, drug thrombolysis, percutaneous coronary intervention, etc., can obviousl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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