Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for pretreating lignocellulose at low temperature through coupling of sodium hydroxide and ethanol

A lignocellulose and low-temperature pretreatment technology, applied in the field of biomass resource utilization, can solve the problems of high pretreatment temperature, achieve the effects of short action time, increase ethanol yield, and increase the yield of enzymatic hydrolysis of raw materials

Inactive Publication Date: 2014-12-24
SOUTHEAST UNIV
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Ethanol pretreatment is currently the most researched organic solvent pretreatment method, including self-catalyzed ethanol pretreatment method and acid-catalyzed ethanol pretreatment method, but these pretreatment methods all have high pretreatment temperature and produce furfural, HMF, etc. fermentation inhibitor

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for pretreating lignocellulose at low temperature through coupling of sodium hydroxide and ethanol
  • Method for pretreating lignocellulose at low temperature through coupling of sodium hydroxide and ethanol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Weigh 10g of corncob after drying and pass through a 40-mesh sieve, add 100ml of a mixed solution containing 0.25mol / L NaOH solution and 50% (v / v) ethanol in a beaker, the reaction temperature is 60℃, and the reaction time is 1h. After the reaction, the slurry is vacuum filtered to achieve solid-liquid separation, and the filter residue produced above is washed with distilled water until the pH value is neutral, and dried at 105° C. to constant weight.

[0036] The pretreated corncob was subjected to Van Soest determination (the main components are as follows: figure 2 Shown) and enzymatic hydrolysis, wherein the enzymatic hydrolysis conditions are: solid-liquid ratio 1:70 (g / mL), time 72h, temperature 50℃, pH 4.8 citric acid-sodium citrate buffer solution, cellulase It is produced by Trichoderma reesei, and its loading is 25FPU / g dry raw material. After enzymolysis, the reaction slurry was taken out, the enzyme was inactivated in a boiling water bath for 10 minutes, an...

Embodiment 2

[0038] Weigh 10g of corncob after drying and pass through a 40-mesh sieve, add 100ml of a mixed solution containing 0.50mol / L NaOH solution and 50% (v / v) ethanol in a beaker. The reaction temperature is 60℃ and the reaction time is 1h. After the reaction, the slurry is vacuum filtered to achieve solid-liquid separation, and the filter residue produced above is repeatedly washed with distilled water until the pH value is neutral, and dried at 105° C. to a constant weight.

[0039] The pretreated corncob was subjected to Van Soest determination (the main components are as follows: figure 2 Shown) and enzymatic hydrolysis, wherein the enzymatic hydrolysis conditions are: solid-liquid ratio 1:70 (g / mL), time 72h, temperature 50℃, pH 4.8 citric acid-sodium citrate buffer solution, cellulase It is produced by Trichoderma reesei, and its loading is 25FPU / g dry raw material. After the enzymolysis, the reaction slurry was taken out, and the enzyme was inactivated in a boiling water bat...

Embodiment 3

[0041] Weigh 10g of corncob after drying and pass through a 40-mesh sieve, add 150ml of a mixed solution containing 0.50mol / L NaOH solution and 50% (v / v) ethanol in a beaker, the reaction temperature is 60℃, and the reaction time is 1h. After the reaction, the slurry is vacuum filtered to achieve solid-liquid separation, and the filter residue produced above is repeatedly washed with distilled water until the pH value is neutral, and dried at 105° C. to a constant weight.

[0042] The pretreated corncob was subjected to Van Soest determination (the main components are as follows: figure 2 Shown) and enzymatic hydrolysis, wherein the enzymatic hydrolysis conditions are: solid-liquid ratio 1:70 (g / mL), time 72h, temperature 50℃, pH 4.8 citric acid-sodium citrate buffer solution, cellulase It is produced by Trichoderma reesei, and its loading is 25FPU / g dry raw material. After the enzymolysis, the reaction slurry was taken out, and the enzyme was inactivated in a boiling water ba...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for pretreating lignocellulose at the low temperature through coupling of sodium hydroxide and ethanol. The method is one of organic solvent methods for pretreating the lignocellulose and comprises steps as follows: the lignocellulose is pretreated with a sodium hydroxide-ethanol mixed solution; after pretreatment, solid-liquid separation is performed on a raw material, solid components are subjected to a cellulose enzymolysis experiment and component analysis after being dried, and liquid components are used for recovering hemicellulose and lignin components. The method aims to weaken hydrogen bonds between cellulose and hemicellulose and ester bonds between saponification hemicellulose and lignin by OH- ions produced by the sodium hydroxide, destroy the compact structure of the lignocellulose and reduce the crystallinity; the raw material of the pretreated lignocellulose contains rich cellulose and part of hemicellulose and is converted into fermentable sugar through cellulose enzymolysis, so that the utilization rate of the raw material of the lignocellulose is increased to the greatest extent. The hemicellulose and the lignin in a pretreating solution are recovered with an acid precipitation method.

Description

Technical field [0001] The invention belongs to the technical field of biomass resource utilization, and relates to organic solvent method pretreatment, using sodium hydroxide-ethanol mixed solution to pretreat lignocellulose. Specifically, it uses OH produced by sodium hydroxide - Ions weaken the hydrogen bond between cellulose and hemicellulose, saponify the ester bond between hemicellulose and lignin, destroy the dense structure of lignocellulose and reduce crystallinity; ethanol can effectively dissolve lignin, which will affect The lignin component of the subsequent enzymatic hydrolysis efficiency is effectively removed from the lignocellulose raw material. This method can greatly reduce the lignin content, shorten the pretreatment time, and improve the efficiency of enzymatic hydrolysis. Background technique [0002] In order to alleviate the increasingly serious energy shortage and environmental pollution problems, countries around the world have begun to actively search ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P19/14
Inventor 仲兆平邓学群艾特玲
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com