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Pseudomonas vranovensis with phosphorus solubilizing capability and application thereof

A technology of Pseudomonas vulgaris and ability is applied in the field of agricultural microorganisms to achieve the effects of simple nutritional requirements, short growth cycle and increased yield

Inactive Publication Date: 2015-02-25
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the modification of microorganisms mainly includes mutagenesis and genetic engineering methods. Microbial fertilizers are directly applied to the soil and the environment with live bacteria. Therefore, the application of genetically engineered bacteria is clearly restricted in my country's bacterial fertilizer standards.
Radiation, nuclear radiation and chemical mutagenesis are commonly used methods of mutation breeding at present, but long-term use of a mutagen will often make strains resistant, and a new mutagenic source must be used in order to obtain greater variation

Method used

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  • Pseudomonas vranovensis with phosphorus solubilizing capability and application thereof
  • Pseudomonas vranovensis with phosphorus solubilizing capability and application thereof
  • Pseudomonas vranovensis with phosphorus solubilizing capability and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Mutagenesis screening of ZSW-3-5-1a

[0035] According to the screening method of the phosphorus-dissolving bacterial strain provided by the present invention, the bacterial strain with strong phosphorus-dissolving ability is selected, and the preparation steps are as follows:

[0036] 1) ZSW-3, which was screened from the plant rhizosphere in the laboratory, has a high phosphorus-solubilizing ability as the starting strain;

[0037] 2) Mutation breeding

[0038] (1) Prepare the single cell suspension of the starting strain ZSW-3

[0039] The starting strain ZSW-3 was inoculated in liquid medium A, cultured at 28-32°C, 150-180rpm for 8-12 hours, centrifuged, washed with sterile saline, placed in a Erlenmeyer flask with glass beads, oscillated, Make it dispersed into a single-cell bacterial suspension;

[0040] The liquid medium A consists of: 5-10 g of tryptone, 3-5 g of yeast powder, 5-10 g of NaCl, 1000 ml of distilled water, and a pH value of 6.8-7.5.

...

Embodiment 2

[0058] Example 2 Determination of ZSW-3-5-1a's ability to decompose calcium phosphate, lecithin and calcium phytate

[0059] Dispense 100ml of liquid culture medium B, C or D into 250ml Erlenmeyer flasks, and sterilize them for later use. Inoculate ZSW-3-5-1a in 50ml of liquid medium A, culture at 28°C for 36 hours, take 100μl (dilute A bacteria solution with liquid medium to make its OD600 0.7) and inoculate it in the above In the liquid culture medium B, C or D, each group was cultured in three parallels at 28°C and 150rpm for 7 days, and the bacteria liquid cultured on the above shaker was taken, centrifuged at 10,000rpm for 10 minutes, and the supernatant was taken and determined by molybdenum-antimony resistance method Phosphorus content in the supernatant. The experimental results showed that the ability of ZSW-3-5-1a to decompose calcium phosphate was 1030±16.2 mg / l, the ability to decompose lecithin was 135±5.42 mg / l, and the ability to decompose calcium phytate was a...

Embodiment 3

[0064] Example 3 Determination of the ability of ZSW-3-5-1a to produce indole acetic acid (IAA)

[0065] Add sterile L-tryptophan with a concentration of 200μg·ml-1 to a 250ml Erlenmeyer flask containing 100ml of liquid medium A, inoculate ZSW-3-5-1a in 50ml of liquid medium A, at 28°C, After culturing at 150rpm for 20 hours, take 100μl (dilute the bacterial solution with medium A to make its OD600 0.7) and inoculate it into the above-mentioned liquid medium A containing L-tryptophan, set up three parallels, 28°C, 150 Rpm cultured for 72 hours, centrifuged at 4000 g for 10 min, and the concentration of IAA in the supernatant was determined by spectrophotometry. The experimental results showed that: ZSW-3-5-1a produced IAA concentration of 44.6±3.2mg / l.

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Abstract

The invention discloses pseudomonas vranovensis with phosphorus solubilizing capability, a preparation method and application thereof. Through an ultraviolet-ray plasma composite mutation technology for multiple composite mutation, a bacterial strain ZSW-3-5-1a with high solubilizing efficiency on phosphorus and good stability is selected and bred, has the classification name of pseudomonas vranovensis, is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No. 8750. The bacterial strain has efficient solubilizing capability on calcium phosphate and calcium phytate, and also is capable of generating a certain amount of indoleacetic acid. By using a liquid containing the bacterium or a solid inocula to inoculate corn, soybean, mustard and other crops, utilization rate on phosphorus in soil is improved and crop output is improved.

Description

technical field [0001] The invention relates to a strain of Pseudomonas vermura with phosphorus-dissolving ability and its application. The strain is obtained by ultraviolet-plasma compound mutagenesis, can improve the utilization rate of soil phosphorus and increase the yield of crops, and belongs to the technical field of agricultural microorganisms. Background technique [0002] Phosphorus is one of the three major elements of plant nutrition. Insufficient phosphorus supply has gradually become one of the most important factors limiting crop yield and quality. The total phosphorus content in the soil is sufficient, containing 0.02-0.5% total phosphorus, but most of them exist in the form of insoluble inorganic phosphates (mainly calcium phosphate, aluminum phosphate, iron phosphate, mainly calcium phosphate in alkaline soil) , only 1% exists in the form of soluble phosphorus salts that plants can directly absorb and utilize. In order to meet the needs of crop growth, nea...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P21/00C05F11/08A01C1/00A01C1/06A01G7/06A01C21/00C12R1/38
CPCA01C1/00A01C21/00A01G7/06A01N63/10C12N1/20C12N1/205C12R2001/38
Inventor 朱晓丽宋进喜马俊杰李贺
Owner NORTHWEST UNIV
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