L-aspartic acid making process

Abstract

The invention discloses an L-aspartic acid making process which comprises the following steps: (1) strain selection; (2) cell immobilization; (3) conversion; (4) modification, to be more specific, dissolving 1g of dextran in 15ml distilled water, dissolving a certain amount of NaIO4 in 10ml hot water, mixing two solutions, reacting at the temperature of 4 DEG C in a refrigerator for 18h, after dialyzing for 4h with distilled water, dialyzing with 5L of 0.1mol / L sodium phosphate buffer fluid with the pH of 7.4, adding the dialyzed liquid into a conversion solution in the step (3), and fully mixing to obtain a mixed liquid; (5) preparation of a crude product; and (6) crystallization; the mass L-aspartic acid can be produced in a short period, after modification, antigenicity is weak, the human body immune response may not be caused, drug efficacy is good, production is fixed, stability is good, and the L-aspartic acid making process is suitable for popularization and application.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a process for preparing L-aspartic acid. Background technique [0002] Aspartic acid, also known as aspartic acid, is an α-amino acid. The L-isomer of aspartic acid is one of the 20 protein amino acids, which are the structural units of proteins. Aspartic acid is ubiquitous in biosynthesis. It is the synthetic precursor of lysine, threonine, isoleucine, methionine and other amino acids and purine and pyrimidine bases in organisms. It can be used as a carrier of K+ and Mg+ ions to transport electrolytes to the myocardium, thereby improving myocardial contractility. , while reducing oxygen consumption, it has a protective effect on the myocardium when there is hypoxia in the coronary artery circulation disorder. It participates in the ornithine cycle, promotes the production of urea from oxygen and carbon dioxide, reduces the amount of nitrogen and carbon dioxide in the blood, enhances...

AI Technical Summary

Problems solved by technology

It participates in the ornithine cycle, promotes the production of urea from oxygen and carbon dioxide, reduces the amount of nitrogen and carbon dioxide in the blood, enhances liver function, eliminates fatigue, and has good medical value, so it is in great demand, forcing its mass production, application number CN200910033427.1 is a method for preparing aspartase and L-aspartic acid by using fumaric acid fermentation broth. The method includes: first, culturing Escherichia coli to produce aspartic acid in the supernatant part of fumaric acid fermentation broth Enzyme, the second is to use the solid precipitation part of the fermentation broth to react with ammonium sulfate to generate ammonium fumarate, and after concentration, L-aspartic acid is generated under the action of aspartase. This method realizes The production of L-aspartic acid by the glycosyl route realizes the full utilization of fumaric acid fermentation broth and achieves the goal of energy saving and emission reduction, which effectively reduces the production cost of L-aspartic acid and reduces environmental pollution. The sustainable production of L-aspartic acid has wide prospects for industrialized production, but the method for producing aspartic acid has a small production capacity, cannot meet a large demand, and has strong antigenicity and weak stability, resulting in Poor efficacy, which limits its scope of use