Phenylboronic Acid Modified Polyethyleneimine Hybrid Magnetic Nanoparticles and Its Preparation and Application

Abstract

The invention belongs to the field of inorganic materials and analysis technology, and in particular relates to a phenylboronic acid-modified polyethyleneimine hybrid magnetic nanoparticle and a preparation method thereof, as well as methods for selectively enriching modified nucleosides and ribosylated metabolites in urine application. The magnetic microspheres are coated with a layer of SiO2 material on the surface of Fe3O4 nanoparticles by reverse phase microemulsion method, and then introduce amino groups through 3-aminopropyltriethoxysilane and then augmented with glutaraldehyde bridge-linked polyethyleneimine. The amount of amino bonds is large, and finally the phenylboronic acid functional group is grafted by reductive amination reaction to form hybrid magnetic nanoparticles. This kind of magnetic material has the characteristics of large adsorption capacity, high selectivity, and short equilibration time, and it is easy to handle and strong in specificity when selectively enriching trace modified nucleosides and ribosylated metabolites in urine. This material shows good prospects in mining cancer markers of novel modified nucleosides or ribosyl compounds and enrichment of other 1,2-cis-dihydroxy compounds.

Description

[0001] Technical field: [0002] The invention belongs to the field of inorganic materials and analysis technology, and in particular relates to a phenylboronic acid-modified polyethyleneimine hybrid magnetic nanoparticle and a preparation method thereof, as well as methods for selectively enriching modified nucleosides and ribosylated metabolites in urine application. [0003] Background technique: [0004] RNA plays a very important role in many life processes. The diverse modifications that occur on the ribose or base of ribonucleic acid are usually closely related to their biological functions. As the terminal metabolite of ribonucleic acid, ribonucleoside can directly reflect the metabolic rate of ribonucleic acid. Due to the lack of specific phosphorylases in cells, modified nucleosides cannot be synthesized and reused like normal nucleosides, so they are usually secreted from cells into body fluids. In cancer cells, impaired transcript RNA metabolism ultimately leads ...

AI Technical Summary

Problems solved by technology

Unfortunately, atom transfer radical reactions usually require strict control of reaction conditions and operate under anhydrous and oxygen-free conditions, and the mesoporous structure usually restricts the transport of internal substances, which is not conducive to non-specific exclusion.

However, the polyethylene glycol chains that are usually used to reduce non-specific adsorption often require multi-step chemical modification in the subsequent introduction of functional groups, which increases the difficulty of synthesis.

Images