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Cordyceps cicadae rhzomorph as well as preparation method and application thereof

A technology of Cicada flower fungus and Paecilomyces cicadae, applied in biochemical equipment and methods, microorganisms, fermentation and other directions, to achieve the effects of simple operation, relatively equipment requirements and clear structure

Active Publication Date: 2015-06-03
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of the above reports are about the fermentation culture method and biological activity of Paecilomyces cicadae, the structure research of polysaccharides in the fruiting body of Paecilomyces cicadae and the polysaccharides in solid medium, and do not involve the advanced structure analysis of the mycelial polysaccharides obtained from the submerged fermentation of Paecilomyces cicadae, such as There are no reports on its monosaccharide composition, glycosidic bond connection, etc.; more and more studies have shown that the important functions of polysaccharides are determined by their structural characteristics, and their higher-order structures (secondary and tertiary structures) are more closely related. , the biological activity of polysaccharides is closely related to its molecular weight and molecular chain conformation (Conformation), understanding the conformation of the sugar molecule is more helpful to clarify the mechanism of its biological activity

Method used

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  • Cordyceps cicadae rhzomorph as well as preparation method and application thereof
  • Cordyceps cicadae rhzomorph as well as preparation method and application thereof
  • Cordyceps cicadae rhzomorph as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0049] (1) submerged fermentation: insert the Paecilomyces cicadae bacterial classification in the culture medium, the inoculum size is 8% (percentage by weight), and under 24 ℃ of conditions, stand and cultivate for 8 days, obtain the Paecilomyces cicadae fermentation mycelium; Wherein The weight percentage of the medium used is composed of: glucose 14%, yeast powder 8%, peptone 5%, cicada pupa powder 0.6%, KH 2 PO 4 0.8%, MgSO 4 .7H 2 O 0.4%, the balance is sterile water.

[0050] (2) Extraction: After pulverizing the fermented mycelia of Paecilomyces cicadae in step (1), add distilled water to form a feed liquid, extract and centrifuge at 85°C, concentrate the obtained extract to obtain a concentrate, and then add 4 times the amount of aqueous ethanol (92% concentration by volume), stirred and mixed, precipitated overnight at 3°C, centrifuged, and the precipitate obtained by centrifugation was taken to obtain the crude polysaccharide of Paecilomyces cicadae mycelia once;...

Embodiment 2

[0056] (1) submerged fermentation: insert the Paecilomyces cicadae bacterial classification in the culture medium, inoculum size 5% (percentage by weight), under 18 ℃ of conditions, static culture 6 days, obtain Paecilomyces cicadae fermentation mycelia; Wherein The weight percentage of the medium used is composed of: glucose 10%, yeast powder 5%, peptone 3%, cicada pupa powder 0.2%, KH 2 PO 4 0.5%, MgSO 4 .7H 2 O 0.1%, the balance is sterile water.

[0057] (2) Extraction: After pulverizing the fermented mycelia of Paecilomyces cicadae in step (1), add distilled water to form a feed liquid, extract and centrifuge at 70°C, concentrate the obtained extract to obtain a concentrate, and then add to account for the volume of the concentrate 5 times the amount of aqueous ethanol (90% concentration by volume), stirred and mixed, precipitated overnight at 2°C, centrifuged, and the precipitate obtained by centrifugation was taken to obtain the crude polysaccharide of Paecilomyces c...

Embodiment 3

[0063] (1) submerged fermentation: insert the Paecilomyces cicadae strain in the culture medium, the inoculum size is 10% (percentage by weight), and at 26°C, leave it to culture for 12 days to obtain the Paecilomyces cicadae fermentation mycelium; The weight percentage of the medium used is composed of: glucose 20%, yeast powder 10%, peptone 9%, cicada pupa powder 1.0%, KH 2 PO 4 1.2%, MgSO 4 .7H 2 O 0.8%, the balance is sterile water.

[0064] (2) Extraction: After pulverizing the fermented mycelia of Paecilomyces cicadae in step (1), distilled water is added to form a feed liquid, extracted and centrifuged at 90°C, the obtained extract is concentrated to obtain a concentrate, and then added to account for the volume of the concentrate 5 times the amount of ethanol aqueous solution (volume percentage concentration is 96%), stirred and mixed, precipitated overnight at 5 ° C, centrifuged, and the precipitate obtained by centrifugation was obtained to obtain the crude polysa...

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Abstract

The invention discloses cordyceps cicadae rhzomorph as well as a preparation method and application thereof. The cordyceps cicadae rhzomorph consists of more than 99 weight percent of polysaccharides; and the polysaccharides comprise mannose, galactose, rhamnose and glucose in a mass ratio of (45.20-50.20):(5.50-8.30):(2.10-2.60):(0.80-1.30). The preparation method of the cordyceps cicadae rhzomorph comprises the steps of extracting crude paecilomyces cicadae mycelium polysaccharides by water extraction and alcohol precipitation, removing protein by an enzyme-Sevage combination method, dialyzing, purifying by anion exchange chromatography and gel filtration chromatography, and performing vacuum freeze drying to separate the purified components, thus obtaining paecilomyces cicadae mycelium polysaccharides, namely cordyceps cicadae rhzomorph. Component analysis, structural identification and immunity researches on the purified polysaccharides show that the paecilomyces cicadae mycelium polysaccharides have relatively strong anti-oxidation activity, can be used as antioxidants or used for preparing antioxidants, and can be widely applied to cosmetics, health-care food, animal feed additives, medicine and the like.

Description

technical field [0001] The invention relates to the field of fungal intracellular polysaccharides, in particular to a cicadaline and its preparation and use, and belongs to the field of macromolecule and molecular biology. Background technique [0002] Cicada flower (Cordyceps cicadae) is a complex of nymphs and nymphs of ergot fungi parasitic on cicada nymphs. Cordyceps is also a nourishing food and has high economic application value (Zhang S.W., Xuan L.J. Five aromatics bearing a 4-O-methylglucose unit from Cordyceps cicadae. Helvetica chimica acta, 2007, 90:404-410.). Paecilomyces cicadae Miq.Samson, also known as female cicadae, is a spore fungus mitotically produced from the fruiting body of cicadae, and is regarded as one of the "Eight Treasures of Traditional Chinese Medicine". my country has a long history of using medicinal fungi such as cicadae as medicinal materials. As early as the Ming Dynasty, "Compendium of Materia Medica" has recorded more than 40 kinds of ...

Claims

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Application Information

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IPC IPC(8): C08B37/00C12P19/04C12R1/79
Inventor 贺亮李卫旗程俊文韩素芳韦朝阳王衍彬钱华陈芸
Owner ZHEJIANG FORESTRY ACAD
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