Application of metarhizium anisopliae strains to preparation of pesticide for killing lissorhoptrus oryzophilus kuschel

A technology for Metarhizium anisopliae and rice water weevil, which is applied in the application, biocide, insecticide and other directions, can solve the problem of wet dispersion characteristics, storage stability, large field control effect, poor wet dispersion effect, and no large-scale application. and other problems, to achieve the effect of suitable for large-scale production and large-scale popularization and use, outstanding effect, good wetting and dispersing characteristics

Inactive Publication Date: 2015-07-01
CHONGQING JULIXIN BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the activity of fungal spores is affected by wetting agents, the dosage of surfactants is often reduced in formulations, resulting in poor wetting and dispersing effects, while the product shelf life is still short, rarely exceeding 12 months
Due to the problem of wet dispersing properties, storage stability and the field control effect is greatly affected by ultraviolet radiation, although the wettable powder of insecticidal fungi has been registered, it has not been widely used.

Method used

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  • Application of metarhizium anisopliae strains to preparation of pesticide for killing lissorhoptrus oryzophilus kuschel
  • Application of metarhizium anisopliae strains to preparation of pesticide for killing lissorhoptrus oryzophilus kuschel
  • Application of metarhizium anisopliae strains to preparation of pesticide for killing lissorhoptrus oryzophilus kuschel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 CQMa421 strain and its virulence to rice water weevil after being inoculated with rice water weevil virulence domestication

[0026] The CQMa421 strain was inoculated on a 1 / 4 SDA plate, cultured at 28°C for 15 days, mature conidia were collected, dispersed with 0.05% (W / V) Tween-80, and prepared to a final concentration of 1×10 7 spores / ml suspension. Collect rice water weevil adults from the wild, and transfer to 500ml triangular flasks with rice seedlings, 50 / bottle. Use a spray tower (POTTER, UK) to spray, inoculate 3 bottles, and inoculate 1.0 ml in each bottle. After inoculation, it is placed in a bioassay room with a room temperature of 28°C and a humidity of 50-70%. Select the rice water weevil that is the first to die, put it in a 28°C incubator and culture it for 3-5 days, so that fresh spores grow on the surface of the insect, and collect the spores; inoculate the rice water weevil 17-19 times as above. domesticated. Under sterile conditions, u...

Embodiment 2

[0033] Example 2 The protective effect of nano-silicon carbide on Metarhizium anisopliae

[0034] According to Table 2, the pure spore powder of Metarhizium anisopliae CQMa421 and nano-silicon carbide were first mixed, and then 40% diatomaceous earth, 5% CMC and different contents of Tween-80 and OP-10 were added and mixed.

[0035] UV protection: disperse the preparation sample with water to make a concentration of 1×10 7 After spores / mL of spore suspension, take 100μl of spore suspension and spread it on 1 / 4 SDA plate, mix well with glass and put it in 290-310nm ultraviolet lamp (978mW / m 2 ) under vertical irradiation for 5 hours, and then cultured in a 28°C incubator for 24 hours to measure the germination rate (the method is the same as above), and repeat 3 times. The data were processed and analyzed with SPSS software.

[0036] Storage experiment: Store the preparation sample in a sealed container at 28°C for 2 months, and disperse it with water to make a concentration ...

Embodiment 3

[0040] Example 3 Preparation of wettable powder of Metarhizium anisopliae CQMa421 and its wettability, storage period and control effect.

[0041] According to Table 3, the pure sporopollen of Metarhizium anisopliae CQMa421 and nano-silicon carbide were first mixed, and then diatomaceous earth, CMC, Tween-80 and OP-10 with different contents were added.

[0042] Determination of wettability of wetting and dispersing agent: Take 100mL of standard water and pour it into a 250mL beaker. Place the beaker in a constant temperature water bath at 25°C±1°C, so that the liquid level is flush with the water bath level. When the hard water reaches 25°C±1°C, accurately weigh 5.0g of the sample to be tested and place it on a watch glass. Do not disturb the liquid level excessively. Immediately record the time with a stopwatch when adding the sample until the sample is completely wetted (the fine powder film remaining on the liquid surface can be ignored). Note down the wetting time (acc...

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Abstract

The invention discloses application of metarhizium anisopliae CQMa421 strains to preparation of a pesticide for killing lissorhoptrus oryzophilus kuschel. Wettable powder produced with the strains consists of kieselguhr (30-40%), nanometer silicon carbide (0.5-1%), sodium carboxymethylcellulose (3-5%), tween-80 (3-5%), OP-10 (3-5%) and metarhizium anisopliae CQMa421 dry spore powder (44-60%). The pesticide for killing the lissorhoptrus oryzophilus kuschel has very good wettability, storage characteristics and uvioresistant characteristics, and the lissorhoptrus oryzophilus kuschel can be effectively controlled.

Description

technical field [0001] The invention relates to a new application of a strain of Metarhizium anisopliae, in particular to its application in the preparation of Metarhizium anisopliae medicament for killing water weevil, and belongs to the field of biological pesticides. technical background [0002] Rice water weevil is a destructive pest of rice. Adults feed on the delicate parts of rice and affect the photosynthesis of rice. The larvae damage the roots, hinder nutrient transmission, slow down the growth of rice seedlings, dwarf plants, and reduce tillering ability, which eventually leads to Rice production decline, generally resulting in 10-30% reduction in production, more than 50% in severe cases, or even no harvest. In 1986, my country listed it as an object of agricultural plant quarantine. However, as of 2011, it has spread to more than 20 provinces, seriously threatening my country's agricultural production and food security. At present, the control of rice water w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01N25/14A01P7/04C12R1/645
CPCA01N25/14A01N63/30C12N1/36A01N2300/00
Inventor 夏玉先彭国雄
Owner CHONGQING JULIXIN BIOENG
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