Trehalose synthase and coding gene and application thereof

Abstract

The invention relates to a trehalose synthase and a coding gene and application thereof. A nucleotide sequence of an expression gene of the trehalose synthase is shown in SEQ ID No. 1; the amino acid sequence of the trehalose synthase is shown in SEQ ID No. 2. According to the trehalose synthase and the coding gene and application thereof, the trehalose synthase is obtained from pseudomonas stutzeri for the first time, and a preparation method of the trehalose synthase is simple and convenient and is high in yield and high in purity; proved by experiments, the thermal stability of the trehalose synthase is good, the trehalose conversion ratio can reach 70% in 1-hour reaction, and the reaction time is greatly shortened compared with that of the existing trehalose synthase, so that the production cost of trehalose can be reduced, and a foundation is laid for the industrial production of trehalose.

Description

technical field

[0001] The invention relates to a trehalose synthase and its encoding gene and application, belonging to the field of biotechnology. Background technique

[0002] Trehalose (Trehalose) is a non-reducing disaccharide composed of two glucopyranose molecules linked by α-1,1-glycosidic bonds, widely present in bacteria, yeast, filamentous fungi, plants, insects, organisms such as vertebrates. Studies have shown that its properties are stable and have very important biological significance to organisms. It is mainly manifested in the fact that it is a reserve of energy and carbon sources for organisms, a stabilizer and protector for proteins and biofilm molecules in harsh environments such as dehydration, high temperature, oxygen free radicals, and low temperature, and a signal-sensing complex and growth agent. It is not only a regulatory factor, but also one of the components of some bacterial cell walls, so trehalose has the reputation of "sugar of life" in th...

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