Dual-color fluorescence quantum dot embedded ratiometric probe and preparation method thereof

Abstract

The invention discloses a dual-color fluorescence quantum dot embedded ratiometric probe and a preparation method thereof; the ratiometric probe takes red quantum dots as a core, the red quantum dots are embedded in silica nanoparticles, then the surface of the red quantum dot embedded silica nanoparticles is subjected to sulfhydrylation, then green quantum dots are grown in-situ on the sulfhydrylation silica surface, and composite nanoparticles with dual emission wavelengths are obtained; finally, the surface of the composite nanoparticles with the dual emission wavelengths is coated with a silica nanoparticle layer, and thus the dual-color fluorescence quantum dot embedded ratiometric probe is obtained. The red ray emitted quantum dot embedded silica nanoparticles are prepared by a reverse microemulsion method; compared with a stober method, the silica nanoparticles have better dispersion; moreover, the quantum dots are made into an alkali solution for incubation, the fluorescence properties are improved, and the silica nanoparticles coating the particle surface improve the specificity and stability of the dual-emission fluorescence ratiometric probe.

Description

(1) Technical field [0001] The invention relates to a fluorescent ratio probe and a preparation method thereof, in particular to a very stable ratio probe embedding two-color fluorescent quantum dots and a preparation method thereof. (2) Background technology [0002] Fluorescent probes have the advantages of safety, convenience, high sensitivity, fast detection speed, simple operation, and good repeatability. In addition to being used for the analysis of some chemical elements, they are also widely used in biology, such as bioimaging, biochips, and protein analysis. , immunofluorescence detection, DNA sequence analysis, etc. At present, most fluorescent probes mainly express information by changing the fluorescence intensity. When used, they often need the assistance of large-scale instruments, resulting in large errors, troublesome operation, and high cost. Fluorescent ratiometric probes are an excellent class of probes. Ratiometric fluorescence signals are preferred ove...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problem of poor dispersibility and stability of the current fluorescent ratio probes, provide a novel ratio probe and its preparation method, and use the inverse microemulsion method to prepare red-light emitting carbon dioxide embedded in quantum dots. Silicon nanoparticles, and in-situ growth of green quantum dot layer, and finally coating it with silicon dioxide to prepare a ratio probe for embedding two-color fluorescent quantum dots; the method of the present invention is simple and easy to implement, the equipment is simple, and the synthesis conditions are mild. The obtained fluorescent ratio probe has good dispersion, good stability, and excellent fluorescence performance. It is a promising fluorescence resonance energy transfer probe and has potential application prospects in biological detection.

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