Dual-color fluorescence quantum dot embedded ratiometric probe and preparation method thereof

A technology of two-color fluorescence and red quantum dots, which is applied in the field of fluorescence ratio probes and its preparation, can solve the problems of dispersion and poor stability of fluorescence ratio probes, and achieve the effect of increasing binding rate, stability and relative content

Active Publication Date: 2015-09-16
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the problem of poor dispersibility and stability of the current fluorescent ratio probes, provide a novel ratio probe and its preparation method, and use the inverse microemulsion method to prepare red-light emitting carbon dioxide embedded in quantum dots. Silicon nanoparticles, and in-situ growth of green quantum dot layer, and finally coating it with silicon dioxide to pre...

Method used

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  • Dual-color fluorescence quantum dot embedded ratiometric probe and preparation method thereof
  • Dual-color fluorescence quantum dot embedded ratiometric probe and preparation method thereof
  • Dual-color fluorescence quantum dot embedded ratiometric probe and preparation method thereof

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Effect test

Embodiment 1

[0041] 1. Preparation of silica nanoparticles embedded with red quantum dots

[0042] 0.2284g cadmium chloride (CdCl 2 2.5H 2 O) Add it to 500mL deionized water, pass nitrogen gas for 20min, then add 0.1mL mercaptopropionic acid, then adjust the pH to 11.00 with 1M aqueous sodium hydroxide solution, then continue to add 0.538g sodium citrate (containing crystal water), 0.0444g Sodium tellurite, 0.2g sodium borohydride, and finally heated to reflux. After refluxing for 8h, obtain the stable cadmium telluride quantum dot aqueous solution (i.e. red quantum dot solution) that emits red fluorescent mercaptopropionic acid, and the emission peak is at 660 nanometers (see attached Figure 6 Middle a curve, see the visualization photo Figure 7 ).

[0043] Take 4mL of the red quantum dot solution synthesized above, add 4mL of absolute ethanol, let it stand for precipitation, centrifuge, and wash the precipitate with absolute ethanol for 1-2 times to obtain red quantum dots. Red qu...

Embodiment 2

[0049] 1. Preparation of silica nanoparticles embedded with red quantum dots

[0050] 0.2284g cadmium chloride (CdCl 2 2.5H 2 O) Add to 500mL deionized water, pass nitrogen gas for 20min, then add 0.1mL mercaptopropionic acid, then adjust the pH to 11.00 with 1M sodium hydroxide, then continue to add 0.538g sodium citrate (containing crystal water), 0.0444g Sodium tellurite, 0.2g sodium borohydride, and finally heated to reflux. After refluxing for 8 hours, an aqueous solution of cadmium telluride quantum dots that emits red fluorescence and is stable with mercaptopropionic acid was obtained.

[0051] Take 6mL of the red quantum dot solution synthesized above, add 6mL of absolute ethanol, let it stand for precipitation, centrifuge, and wash the precipitate with absolute ethanol for 1-2 times to obtain red quantum dots. Disperse 0.0015g of red quantum dots in a mixed solution formed by 0.016ml of ammonia water (25wt%) and 0.0006g of sodium hydroxide. The final concentration ...

Embodiment 3

[0057] 1. Preparation of red light emitting silica nanoparticles embedded with quantum dots

[0058] 0.2284g cadmium chloride (CdCl 2 2.5H 2 O) Add to 500mL deionized water, pass nitrogen gas for 20min, then add 0.1mL mercaptopropionic acid, then adjust the pH to 11.00 with 1M sodium hydroxide, then continue to add 0.538g sodium citrate (containing crystal water), 0.0444g Sodium tellurite, 0.2g sodium borohydride, and finally heated to reflux. After refluxing for 8 hours, an aqueous solution of cadmium telluride quantum dots that emits red fluorescence and is stable with mercaptopropionic acid was obtained.

[0059] Take 4mL of the red quantum dot solution synthesized above, add 4mL of absolute ethanol, let it stand for precipitation, centrifuge, and wash the precipitate with absolute ethanol for 1-2 times to obtain red light quantum dots. Disperse 0.001g of red light quantum dots in a mixed solution formed by 0.016ml of ammonia water (25wt%) and 0.0006g of sodium hydroxide...

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Abstract

The invention discloses a dual-color fluorescence quantum dot embedded ratiometric probe and a preparation method thereof; the ratiometric probe takes red quantum dots as a core, the red quantum dots are embedded in silica nanoparticles, then the surface of the red quantum dot embedded silica nanoparticles is subjected to sulfhydrylation, then green quantum dots are grown in-situ on the sulfhydrylation silica surface, and composite nanoparticles with dual emission wavelengths are obtained; finally, the surface of the composite nanoparticles with the dual emission wavelengths is coated with a silica nanoparticle layer, and thus the dual-color fluorescence quantum dot embedded ratiometric probe is obtained. The red ray emitted quantum dot embedded silica nanoparticles are prepared by a reverse microemulsion method; compared with a stober method, the silica nanoparticles have better dispersion; moreover, the quantum dots are made into an alkali solution for incubation, the fluorescence properties are improved, and the silica nanoparticles coating the particle surface improve the specificity and stability of the dual-emission fluorescence ratiometric probe.

Description

(1) Technical field [0001] The invention relates to a fluorescent ratio probe and a preparation method thereof, in particular to a very stable ratio probe embedding two-color fluorescent quantum dots and a preparation method thereof. (2) Background technology [0002] Fluorescent probes have the advantages of safety, convenience, high sensitivity, fast detection speed, simple operation, and good repeatability. In addition to being used for the analysis of some chemical elements, they are also widely used in biology, such as bioimaging, biochips, and protein analysis. , immunofluorescence detection, DNA sequence analysis, etc. At present, most fluorescent probes mainly express information by changing the fluorescence intensity. When used, they often need the assistance of large-scale instruments, resulting in large errors, troublesome operation, and high cost. Fluorescent ratiometric probes are an excellent class of probes. Ratiometric fluorescence signals are preferred ove...

Claims

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Application Information

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IPC IPC(8): C09K11/88C09K11/02G01N21/64
Inventor 汪晶蒋晨星胡军王力耕
Owner ZHEJIANG UNIV OF TECH
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