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Preparation and application for magnetic metal organic framework medium modified by nucleic acid aptamer

A nucleic acid aptamer and magnetic metal technology, which is applied in the field of material science and modern separation and analysis, can solve the problems of poor selectivity and solvent resistance, and achieve the effect of high extraction capacity and good selective enrichment ability

Inactive Publication Date: 2015-11-18
兴义民族师范学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to their porous, tunable pore size, large specific surface area, easy modification, and metal ion centers with unsaturated coordination, MOFs materials are more expected by scholars in the field of sample pretreatment, and are worthy of further research. Solvent resistance is poor, while MIL-101 has good solvent resistance and has a good application prospect in pretreatment, but its adsorption performance mainly depends on non-specific adsorption between media, and its selectivity is poor, so it is urgent to modify it To meet the requirements of high selective pretreatment and high sensitivity and rapid detection of complex samples

Method used

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  • Preparation and application for magnetic metal organic framework medium modified by nucleic acid aptamer
  • Preparation and application for magnetic metal organic framework medium modified by nucleic acid aptamer
  • Preparation and application for magnetic metal organic framework medium modified by nucleic acid aptamer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Preparation of magnetic MIL-101 modified by nucleic acid aptamer

[0030] The synthesis schematic diagram of OTAApt-MMIL-101 of the present invention is as follows figure 1 Shown:

[0031] (1)NH 2 -Fe 3 O 4 Preparation: accurately weigh 28.1gFeCl 3 ·6H 2 O, 14.4gFeSO 4 ·7H 2 O, dissolved in 150mL distilled water, poured into a 500mL three-necked flask, mechanically stirred, and deoxygenated by nitrogen for 10min. Quickly add 70 mL of 25% ammonia water dropwise, then drop 10 mL of oleic acid into the flask at a constant rate of 0.5 mL / min, and stir at constant temperature for 1 hour. Put the obtained magnetic fluid on a magnet and settle for 20 minutes, then pour the supernatant liquid, and use de-distilled water to ultrasonically clean the magnetic separation. Measure 15 mL of magnetic fluid and place it in 200 mL of n-propanol. After sonicating for 15 minutes, add NH under mechanical stirring. 3 ·H 2 O (25%) (5.0 mL), TEOS (2.0 mL) was continuously added, and kep...

Embodiment 2

[0035] Example 2 The selectivity and enrichment ability of OTAApt-MMIL-101

[0036] (1) Selectivity

[0037] OTAApt-MMIL-101 has specific recognition performance for its target. Six analogues of ochratoxin A are selected, ochratoxin B (Ochratoxin B, OTB), aflatoxin B1, aflatoxin G1, and Aflatoxin G2, methaquine and quinenone. The adsorption capacity of OTAApt-MMIL-101 for different compounds was evaluated by the MSPE extraction concentration of 10μg / L absolute adsorption capacity. A standard solution of 10 μg / L of each substance was prepared with hydroxyethylpiperazine ethanesulfonic acid (HEPES) buffer solution, and the extraction volume was 10 mL. The results are shown in Table 1.

[0038] Table 1 The adsorption performance of OTAApt-MMIL-101 for different substances

[0039]

[0040] As shown in Table 1, the OTAApt-MMIL-101 prepared by the present invention has a high selective extraction capacity for ochratoxin A (OTA). For the structural analogues of OTA, such as ochratoxin B,...

Embodiment 3

[0042] Example 3 Application of OTAApt-MMIL-101 combined with HPLC-FLD to analyze trace ochratoxin in food

[0043] (1) Establishment of HPLC-FLD analysis method

[0044] Prepare a series of ochratoxin A standard solutions with a concentration gradient of 20.0, 10.0, 2.00, 1.50, 1.00, 0.500, 0.100, 0.0500, 0.0250μg / L, etc., and modify the magnetic MIL- with ochratoxin A aptamer 101 solid phase extraction combined with UPLC-FLD to determine the standard curve, linear range, correlation coefficient and detection limit of ochratoxin A. Within the set concentration range, the standard equation of ochratoxin A is Y=3.71×10 5 X+1.14×10 4 , The linear relationship is good at 0.025-20μg / L (correlation coefficient is 0.9998), and the precision of the method is investigated using 1μg / L standard solution (n=5) RSD is 3.2%, the detection limit is 6.7ng / L (the detection limit is The signal-to-noise ratio is estimated at 3:1).

[0045] (2) Analyze actual samples

[0046] Weigh 10.0g powder of cru...

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Abstract

The present invention belongs to the field of materials science and modern separation and analysis, and relates to a preparation method and an application for a magnetic metal organic framework medium modified by a new nucleic acid aptamer. The method comprises the following steps of: firstly preparing nanoscale amino functional magnetic ferroferric oxide (NH2-Fe3O4); then adopting a hydrothermal synthesis method to obtain the magnetic metal organic framework medium; and finally adopting a coupling agent streptavidin as an intermediate to prepare the magnetic metal organic framework medium modified by an ochratoxin A nucleic acid aptamer (OTA Apt -MMIL-101). With adoption of reaction functionalization of ferroferric oxide and 3-aminopropyl triethoxysilane (APTES), OTA Apt-MMIL-101 is synthesized by the NH2-Fe3O4 and a metal organic framework 101 medium (MIL-101) synthetic agent under a condition of hydrothermal reaction through a chemical bonding method, is washed and is dried in vacuum. The OTA Apt-MMIL-101 disclosed by the present invention has good stability and high selectivity recognition performance, and is applicable to selective enrichment and separation of complex samples such as organisms, environment and food.

Description

Technical field [0001] The invention belongs to the field of material science and modern separation analysis, and relates to the preparation of a novel magnetic MIL-101 and a modified nucleic acid aptamer. The magnetic solid-phase extraction medium has high specific recognition performance for specific ochratoxin A, and is suitable for the selective separation and enrichment of complex samples such as biology, environment and food. Background technique [0002] For the analysis of trace and ultra-trace components in complex biological samples, the complex matrix and low content of analysis objects make sample pretreatment extremely difficult. Simple, fast, efficient, green and highly selective sample preparation technology is an important step for the analysis of trace or ultra-trace components in complex samples such as plasma, urine, environmental samples and food. More and more attention and attention has been paid to sample pretreatment technologies, such as solid phase extr...

Claims

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Application Information

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IPC IPC(8): B01J20/286B01J20/28B01J20/30
Inventor 张仟春郭璇陈明华易君明龙巍然张国义
Owner 兴义民族师范学院
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