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Sansevieria trifasciata Prain flavones, and preparation method and application thereof

A kind of tiger Pilan flavonoids, the technology of blue flavonoids, applied in the field of tiger Pilan flavonoids and preparation thereof, can solve the problems such as reports or patent application documents that have not yet seen flavonoid components and their efficacy research

Inactive Publication Date: 2015-12-16
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the fact that there are no reports or patent application documents on the flavonoid components and their efficacy research in Hupilan

Method used

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  • Sansevieria trifasciata Prain flavones, and preparation method and application thereof
  • Sansevieria trifasciata Prain flavones, and preparation method and application thereof
  • Sansevieria trifasciata Prain flavones, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The extraction process optimization of embodiment 1 tiger Piran flavonoids

[0048] (1) Standard curve preparation

[0049] Weigh 0.0050 g of rutin standard substance that is fully dried and constant weight, dissolve it with ethanol with a concentration of 60%, and set the volume to 50 mL to obtain a rutin standard solution with a concentration of 0.1 mg / mL. Take the above standard solution 0.0mL, 1.0mL, 2.0mL, 3.0mL, 4.0mL, 5.0mL in six 10mL graduated test tubes, make up to 5.0mL with 60% ethanol, add 0.3mL 5% (W / V) sodium nitrite Solution, shake well, add 0.3mL10% (W / V) aluminum nitrate solution after standing for 6min, stand for 6min, then add 4.0mL1mol / L sodium hydroxide solution, mix well, dilute to the mark with 60% ethanol (add 0.4mL60 % ethanol), put it at room temperature for 15 minutes, measure its absorbance, take 60% ethanol as the blank control and add the above-mentioned solvent, and draw a standard curve, such as figure 1 .

[0050] (2) Calculation met...

Embodiment 2

[0061] The purification of embodiment 2 Tiger Piran flavonoids

[0062] (1) Preparation of tiger piran flavonoids

[0063] Add 2 times the volume of petroleum ether to the tiger Pilan ultrasonic extract described in Example 1, extract several times, discard the petroleum ether layer solution when the extraction layer is close to colorless, rotate the flavonoid extract after decolorization by rotary evaporation, and volatilize ethanol, Add 1 times the volume of ethyl acetate to the remaining solution for extraction and collect the ethyl acetate layer. After repeated extractions, the combined ethyl acetate is evaporated on a water bath.

[0064] (2) Silica gel column purification of tiger piran flavonoids

[0065] Put 400g of silica gel in a beaker, place it in an oven at 120°C for activation for 5 hours, and seal and store the activated silica gel for later use. Dissolve the activated silica gel in chloroform, keep stirring in the same direction to remove air bubbles, use a...

Embodiment 3

[0066] Example 3 Establishment of the HPLC detection method for tiger piran flavonoids

[0067] (1) Qualitative detection of tiger piranha flavonoids

[0068] Measure a small amount of rutin 1, rutin 2, quercetin, and kaempferol standard substances and dissolve them in 10 mL of methanol as a control solution. Take 0.2 g of tiger piran flavone and dissolve it in 10 mL of methanol solution. Take 6 μL each of the control solution and the test solution and spot on the same silica gel G thin-layer plate. The developer is ethyl acetate: formic acid: water = 8:1:1 and the developer is benzene: ethyl acetate: formic acid = 8:3:1. After the unfolding, dry the thin-layer board, spray it evenly with 1% aluminum trichloride, dry it in a fume hood, and observe it at 254nm. The results show that when the developer is ethyl acetate:formic acid:water=8:3:1, the polarity is too large, as image 3 As shown, the rutin band can be run out, but both quercetin and kaempferol run to the front,...

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Abstract

The invention discloses Sansevieria trifasciata Prain flavones which are prepared by the following steps: by using fresh Sansevieria trifasciata Prain as the raw material, pretreating to obtain dry Sansevieria trifasciata Prain powder; carrying out ultrasonic ethanol extraction; extracting with petroleum ether and ethyl acetate; and purifying by silica gel chromatography to obtain the four components TFS-a, TFS-b, TFS-c and TFS-d of the Sansevieria trifasciata Prain flavones. The Sansevieria trifasciata Prain flavones have obvious inhibiting actions on Salmonella and Escherichia coli, and the MIC (minimal inhibitory concentration) is respectively 3.75 mg / mL. The Sansevieria trifasciata Prain flavone TFS-b has obvious removal actions on hydroxy free radicals, DPPH free radicals and superoxide anions (respectively 59.8%, 78.6% and 84.2%). The Sansevieria trifasciata Prain flavones TFS-c and TFS-d have obvious inhibiting actions on growth of human cervical cancer cell line Hela and mouse myeloma cell line SP2 / 0, and do not have significant difference for growth of human lung cancer cell line A549, laryngocarcinoma cell line Hep-2 and normal cell line DK.

Description

technical field [0001] The invention belongs to the field of biological technology, and in particular relates to saffron flavonoids and a preparation method and application thereof. Background technique [0002] Tiger Pilan, Liliaceae, Sansevieria genus, native to West Africa, strong adaptability to the environment, strong leaf fibers, can be used for weaving. At present, it is planted all over our country and can be used as an indoor foliage plant for potting. Because it can absorb indoor formaldehyde very well and can purify the air, it is called "natural scavenger". Studies have shown that Tiger Piran contains macroelements calcium 172.85mg / g, magnesium 42.87mg / g, potassium 0.1212mg / g, sodium 0.0687mg / g, and trace elements iron 0.1066mg / g, manganese 0.0770mg / g, copper 0.0073mg / g, zinc 0.0040mg / g, and the average mass fraction of essential amino acids contained in the human body is 2.384%. Twelve steroidal saponins were isolated from Sansevieria by means of gas chromatogr...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07H1/08A61K31/7048A61P31/04A61P39/06A61P35/00
CPCY02A50/30
Inventor 李泽鸿刘莹钟月姣张继元刘树英赵福广张文慧刘洪章马丽华薛英刘楚含刘回民
Owner JILIN AGRICULTURAL UNIV
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