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A shape-memory highly elastic active nanofiber scaffold and its application

A nanofiber, high-elasticity technology, applied in the field of preparation of bioabsorbable shape memory biomaterials, can solve the problems of mismatched biomechanical properties, biocompatibility and insufficient anticoagulation, and achieve good application prospects

Inactive Publication Date: 2018-07-17
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Aiming at the problems of existing tissue engineering scaffold materials as soft tissue engineering scaffold materials with mismatched biomechanical properties and insufficient biocompatibility and anticoagulant properties, the primary purpose of the present invention is to provide a shape memory type highly elastic active nano Fiber scaffold, the shape memory type highly elastic active nanofiber scaffold dissolves the linear polyester Poly(CL-co-TOSUO) (abbreviation: linear elastic polyester PCT) with independent property rights of shape memory behavior and elastic control in the spinning Silk solvent, using electrospinning process, or combining linear elastic polyester PCT with other biologically active molecules (such as: L-arginine, albumin, heparin, phosphorylcholine, short peptide, CD34+, VEGF, Chitosan, hydroxyapatite, etc.) were electrospun to prepare

Method used

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  • A shape-memory highly elastic active nanofiber scaffold and its application
  • A shape-memory highly elastic active nanofiber scaffold and its application
  • A shape-memory highly elastic active nanofiber scaffold and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] (1) Take 2g of linear elastic polyester PCT 8-2 with an electronic balance and dissolve it in 18g of dichloromethane: in the mixed solvent of hexafluoroisopropanol=50:50, stir until completely dissolved to obtain linear elastic polyester PCT 8-2 PCT8-2 spinning solution with a final concentration of 10% (w / w).

[0065] (2) Select a 5mL syringe and a needle with an inner diameter of 1.2mm to extract the PCT 8-2 spinning solution with a concentration of 10% (w / w) obtained in step (1), and fix it on the electrospinning device for electrospinning Silk, the process parameters are: the spinning solution injection rate is 2mL / h, the voltage is 15kV, the receiving distance is 20cm, the ambient temperature is 22°C, and the ambient relative humidity is 40%. Aluminum foil was used as the receiving device, and the fiber membrane was spun for 2 hours, and the collected fiber membrane was dried in a vacuum oven at room temperature for 24 hours to obtain a shape-memory highly elastic ...

Embodiment 2

[0068] (1) Weigh 2g of linear elastic polyester PCT 9-1 with an electronic balance and dissolve it in 16g of DMF: trifluoroacetic acid = 50:50 mixed solvent, stir until completely dissolved to obtain linear elastic polyester PCT 9-1 PCT9-1 spinning solution with a final concentration of 8% (w / w).

[0069] (2) Select a 5mL syringe and a needle with an inner diameter of 1.2mm to extract the PCT 9-1 spinning solution with a concentration of 8% (w / w) obtained in step (1), and fix it on the electrospinning device for electrospinning Silk, the process parameters are: the spinning solution injection rate is 2mL / h, the voltage is 15kV, the receiving distance is 20cm, the ambient temperature is 22°C, and the ambient relative humidity is 40%. Aluminum foil was used as the receiving device, and the fiber membrane was spun for 2 hours, and the collected fiber membrane was dried in a vacuum oven at room temperature for 24 hours to obtain a shape-memory highly elastic active nanofiber scaff...

Embodiment 3

[0071] Example 3: Heparin Coaxial Spinning Stent Loaded with Growth Factors

[0072] (1) Weigh 2g of linear elastic polyester PCT 8-2 with an electronic balance and dissolve it in 16g of dimethyl sulfoxide: trifluoroacetic acid = 50:50 mixed solvent, stir until completely dissolved, and obtain linear elastic polyester PCT 8-2 PCT spinning dope A with a final concentration of 8% (w / w), used as the core layer for coaxial spinning.

[0073] (2) 400 mg of heparin, 600 mg of type I collagen and 1000 mg of linear elastic polyester PCT8-2 were dissolved in 16 g of hexafluoroisopropanol to obtain a final concentration of linear elastic polyester PCT 8-2 of 8% (w / w ) of heparin / collagen / elastic polyester spinning dope B, as the shell layer of coaxial spinning.

[0074] (3) Using coaxial spinning needles, the two kinds of spinning solutions were fixed on the electrospinning device through different syringe pumps for electrospinning. The process parameters were: the advancing speed of t...

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Abstract

The invention discloses a shape memory type high-elasticity activity nano-fiber stent and application thereof. The shape memory type high-elasticity activity nano-fiber stent is manufactured by introducing biological activity components (laminin, heparin, CD34+, VEGF and the like) into degradable linear elasticity polyester PCT with a shape memory effect through an electrostatic spinning technology; the molecular weight of the linear elasticity polyester PCT is 50000-400000, and the percentage composition of functionality caprolactone monomers, containing side cyclic ether substitutes, in a copolyester is 5-50%. The shape memory type high-elasticity activity nano-fiber stent is a high-activity nano-fiber stent with human body biomechanics elasticity, is a three-dimensional degradable tissue engineering stent which can provide an ideal biomechanics environment and an ideal biological activity microenvironment for cell growth, and is expected to be widely applied to regeneration and repair of elastic human body tissue such as blood vessels, cardiac muscles, nerves, skin, tendon, heart and the like.

Description

technical field [0001] The invention belongs to the field of preparation of bioabsorbable shape-memory biomaterials, and in particular relates to a shape-memory high-elastic active nanofiber scaffold and its application. Background technique [0002] Extensive research on tissue engineering has made people gradually realize that the mechanical properties of scaffold materials have an important impact on cell growth. Existing studies have shown that certain mechanical stimuli can promote cell growth, and the mechanical compliance of scaffold materials corresponding to human biomechanics Sexuality determines the success or failure of tissue regeneration and repair. The mechanical compliance of materials suitable for human tissue can promote tissue regeneration and gradually transfer stress to the remodeled natural tissue during tissue regeneration. Matching mechanical properties can lead to graft failure. In the study of tissue-engineered small-caliber blood vessels, it was f...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/18A61L27/20A61L27/50A61L27/60A61L27/54D01F6/92D01F1/10D04H1/728
CPCA61L27/18A61L27/20A61L27/50A61L27/54A61L27/60A61L2300/236A61L2300/252A61L2300/414A61L2300/42A61L2400/16A61L2430/10A61L2430/32D01F1/10D01F6/92D04H1/728C08L5/08C08L67/04
Inventor 王小莺林卉恒王剑金汤顺清武征何留民罗丙红
Owner JINAN UNIVERSITY
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