A method for producing butanol by enzymatic hydrolysis and fermentation of lignocellulose

A lignocellulosic enzyme and lignocellulose technology, applied in the direction of microorganism-based methods, methods using microorganisms, fermentation, etc., can solve the problems of tediousness, large sugar loss, and long time consumption, and achieve the goal of reducing interference and reducing inhibition Effect

Active Publication Date: 2019-10-15
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many detoxification methods for lignocellulosic raw material hydrolyzed sugar solution, mainly including excessive lime neutralization, activated carbon adsorption, ion exchange, steam stripping and biological detoxification, etc., but these methods have poor detoxification effect, high cost, or Disadvantages such as large loss of sugar while inhibiting the removal of substances
[0005] CN200910088002.X discloses a method for steam-exploded straw xylose fermentation of acetone butanol and extraction of residues, and reports a method for removing inhibitors by using resins (including macroporous adsorption resins and basic ion exchange resins), so that after detoxification The steam-exploded straw enzymatic solution can be fermented with acetone butanol, and the final total solvent output reaches 12-22g / L, but detoxification by adsorption is expensive, and the loss of reducing sugar in the enzymatic solution is very large, so it has to be processed again The enzymatic solution is concentrated, the whole process is time-consuming and costly
[0006] CN101748158 discloses a method for preparing bio-butanol by fermenting lignocellulosic biomass as raw material. Corn stalks are pretreated with 2% sulfuric acid (v / v), and calcium hydroxide (milk of lime) neutralizes the treatment solution. Concentrate the treatment solution with an organic ultrafiltration membrane to obtain high-concentration sugar, and then detoxify it with a macroporous adsorption resin, then ferment the treatment solution with Clostridium acetobutylicum, and finally obtain a total solvent of 15.6 g / L. Although this process eliminates the need for fiber Sulfase treatment step, but cumbersome and expensive treatment and detoxification steps are not conducive to practical industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Control the total mass of the enzymatic hydrolysis system to 2kg, add pretreated corn stalks (PCS) according to the dry matter concentration of 15wt%, control the amount of enzyme addition to 7IU / g cellulose dry basis, and add 0.05mol / L butyl acetate at the same time as Extractant for enzymatic hydrolysis. The pH value of the enzymatic hydrolysis is 4.5, and the temperature is 50°C. After 30 hours of enzymatic hydrolysis, calcium hydroxide solid particles are added to adjust the pH of the enzymatic hydrolysis solution to 6.0, and then centrifuged to obtain the detoxified enzymatic hydrolysis solution. Liquid was added to the reactor, oxygen scavenger was added and high-purity nitrogen gas was introduced to maintain anaerobic environment, and then P2 medium was added (in g / L: yeast 1.0, CH 3 COONH 4 2.2, KH 2 PO 4 0.5,K2 HPO 4 0.5, MnSO 4 0.01, NaCl 0.01, MgSO 4 ·7H 2 O 0.2, FeSO 4 0.01, p-aminobenzoic acid 0.001, vitamin B 1 0.001, biotin 0.01×10 -3 ), an...

Embodiment 2

[0030] Control the total mass of the enzymatic hydrolysis system to 2kg, add pretreated corn stalks (PCS) according to the dry matter concentration of 20wt%, control the amount of enzyme added to 10IU / g cellulose dry basis, and add 0.08mol / L butyl acetate at the same time as Extractant for enzymatic hydrolysis reaction. The pH value of the enzymatic hydrolysis is 5.0, and the temperature is 52°C. After 20 hours of enzymatic hydrolysis, calcium hydroxide solid particles are added to adjust the pH of the enzymatic hydrolysis solution to 7.0, and then centrifuged to obtain the detoxified enzymatic hydrolysis solution. Liquid was added to the reactor, oxygen scavenger was added and high-purity nitrogen gas was introduced to maintain anaerobic environment, and then P2 medium was added (in g / L: yeast 1.0, CH 3 COONH 4 2.2, KH 2 PO 4 0.5,K 2 HPO 4 0.5, MnSO 4 0.01, NaCl 0.01, MgSO 4 ·7H 2 O 0.2, FeSO 4 0.01, p-aminobenzoic acid 0.001, vitamin B 1 0.001, biotin 0.01×10 ...

Embodiment 3

[0032] Control the total mass of the enzymatic hydrolysis system to 2kg, add pretreated corn stalks (PCS) according to the dry matter concentration of 30wt%, control the amount of enzyme addition to 20IU / g cellulose dry basis, and add 0.1mol / L butyl acetate at the same time as Extractant for enzymatic hydrolysis reaction. The pH value of the enzymolysis is 5.5, the temperature is 48°C, after 25 hours of enzymolysis, calcium hydroxide solid particles are added to adjust the pH of the enzymolysis solution to 8.0, and then centrifuged to obtain the detoxified enzymolysis solution, and the detoxification enzymolysis solution Liquid was added to the reactor, oxygen scavenger was added and high-purity nitrogen gas was introduced to maintain anaerobic environment, and then P2 medium was added (in g / L: yeast 1.0, CH 3 COONH 4 2.2, KH 2 PO 4 0.5,K 2 HPO 4 0.5, MnSO 4 0.01, NaCl 0.01, MgSO 4 ·7H 2 O 0.2, FeSO 4 0.01, p-aminobenzoic acid 0.001, vitamin B 1 0.001, biotin 0....

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Abstract

The invention discloses a method used for producing butanol via enzymolysis and fermentation of lignocelluloses. The method comprises following steps: (1) lignocelluloses obtained via pretreatment, cellulose, and butyl acetate are delivered into a reactor at a certain ratio for enzymolysis; (2) pH value of an obtained enzymolysis liquid is adjusted to 6 to 8 with calcium hydroxide, and reaction is carried out for 1h at 50 DEG C; (3) a deoxidant is added into the enzymolysis liquid, and an inert gas is added so as to obtain an oxygen-free system; and (4) the enzymolysis obtained via detoxification is inoculated with a butanol fermentative strain, and butanol is prepared via fermentation. According to the method, an appropriate extraction agent and an appropriate detoxifier are selected for in-suit extraction separation of fermentation inhibitors such as acetic acid, furfural, and hydroxymethylfurfural generated in pretreatment and enzymolysis processes, toxicity of the inhibitors on enzymolysis and fermentation is reduced, and butanol yield is increased.

Description

technical field [0001] The invention belongs to the field of biomass energy, and in particular relates to a method for producing butanol by enzymatic hydrolysis and fermentation of lignocellulose. Background technique [0002] As an important four-carbon platform compound, butanol has a very wide range of uses, mainly used in butyl acetate, n-butyl phthalate (DBP), butyl acrylate, fatty dibasic acids and phosphoric acid plasticizers and so on. Butanol is also a new type of biofuel with great potential, known as the second-generation biofuel, which is superior to ethanol in replacing gasoline as fuel, as shown in: the calorific value contained in butanol is 25% higher than that of ethanol. Quite; the flash point of butanol is higher than that of ethanol, and it is safer to use; compared with ethanol, butanol is more soluble in gasoline and diesel, but not easily soluble in water; butanol is less corrosive, easy to transport, and can be directly applied to automobiles without...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/22C12P7/16C12P19/14C12R1/145
Inventor 关浩张全曹长海唐似茵
Owner CHINA PETROLEUM & CHEM CORP
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