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Electrochemistry sensor for measuring organophosphorus pesticide residues and measurement method thereof

A technology of organophosphorus pesticides and detection methods, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of unfavorable repeatability and reliability, difference in selectivity and activity, and inability to realize on-site detection, etc., to achieve enhanced stability Reproducibility and repeatability, short detection time, and the effect of saving detection time

Inactive Publication Date: 2017-02-01
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chromatographic analysis methods require large-scale analytical instruments, the process is cumbersome, and on-site detection cannot be realized; the enzyme inhibition method occupies a place in the field of rapid detection of organic phosphorus due to the advantages of miniaturization of instruments and short detection time.
However, due to the variety of enzymes used, their selectivity and activity are very different, which has a negative impact on the repeatability and reliability of the actual detection.

Method used

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  • Electrochemistry sensor for measuring organophosphorus pesticide residues and measurement method thereof
  • Electrochemistry sensor for measuring organophosphorus pesticide residues and measurement method thereof
  • Electrochemistry sensor for measuring organophosphorus pesticide residues and measurement method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Preparation of sensor for electrochemical detection of organophosphorus pesticide residues

[0040] (1) Preparation of mercaptoethylamidophosphamide (TMA)

[0041] Dissolve methamidophos (0.1g) in 10mL hydrochloric acid (0.1mol / L), and stir for 1h under ice-bath conditions to prepare the hydrochloric acid solution of methamidophos; N-bromosuccinimide (NHS, 0.542g ) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC, 0.254g) were dissolved in phosphate buffer and stirred at room temperature for 3 hours, then slowly added mercapto Acetic acid (use sodium bicarbonate solution to adjust the pH to neutral) solution, continue to stir for 2 hours, add the resulting reactant to the hydrochloric acid solution of methamidophos and continue to stir at room temperature for 10 hours, then rotary evaporate most of the solvent at room temperature , a large amount of white solid was precipitated. Filter under reduced pressure and wash with a small amount o...

Embodiment 2

[0043] The independent detection of embodiment 2 organophosphorus standard sample

[0044] In this example, the organophosphorus standard samples are tested separately, and the organophosphorus pesticide samples are selected from fenthion, trichlorfon, dichlorvos, chlorpyrifos, parathion, diazinon, methamidophos, pirimiphos-methyl, dimethoate , phoxim and omethoate.

[0045] The electrochemical sensors prepared in Example 1 were respectively immersed in organic phosphorus concentrations of 0ng / mL, 1ng / mL, 10ng / mL, 50ng / mL, 100ng / mL, 200ng / mL, 400ng / mL, 600ng / mL, 800ng / mL, 1200ng / mL and 1500ng / mL PBS buffer solution (which all contain AchE at a concentration of 10μg / mL) for incubation, rinsed with PBS buffer solution and placed in a place containing K 3 [Fe(CN) 6 Carry out square wave voltammetry (SWV) scan in the PBS buffer solution of ], record response current; The response current of blank standard sample is I 0 , the response current of the standard sample containing o...

Embodiment 3

[0050] The standard addition detection of dichlorvos in embodiment 3 apples

[0051] Weigh the cleaned apple samples (three parallel samples, each with a mass of 50±0.005g) and put them into a beaker, add dichlorvos standard solution, then add 50mL water and 100mL acetone, grind and extract with a grinder for 3min. After filtration, take 100 mL of filtrate and place it in a separatory funnel, add 15 g of sodium chloride, shake vigorously for 3 minutes and then let it stand still. After the solution is separated, extract the aqueous phase with 50 mL of dichloromethane, and combine the acetone and dichloromethane extracts. Dry with anhydrous sodium sulfate to remove the water, concentrate by rotary evaporation to about 2mL, blow dry the remaining liquid with nitrogen, and finally add 10mL ethanol-water solution (V:V=1:1), shake until dissolved, store in low temperature and shade for later use .

[0052] A blank sample without adding dichlorvos was prepared according to the abov...

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Abstract

The invention discloses an electrochemistry sensor for measuring organophosphorus pesticide residues and measurement method thereof. The surface of the electrochemistry sensor is decorated with gold nanoparticles, the sulfydryl methylacetamide phosphoramide molecules are automatically assembled and connected with the gold nanoparticle surface through an Au-S key. Based on the principle that acetylcholin esterase can have strong interactions with organophosphorus, the electrochemistry sensor provides an electrochemistry measuring method that requires no enzyme catalysis. The sensor uses short measurement time, can be used for single and gross measurements of organophosphorus.

Description

technical field [0001] The invention belongs to the technical field of food safety detection and analysis, and relates to an electrochemical detection sensor and a detection method that can be used for the detection of organophosphorus pesticide residues. Background technique [0002] my country is a country with a large population and a large agricultural country. The annual output of imported and exported agricultural products ranks first in the world. Organophosphorus pesticides have a wide insecticidal spectrum, diversified insecticidal methods, many varieties, high efficacy, easy degradation, short residual time, and pertinence. Strong and other characteristics, it has become a kind of insecticide with the widest range and the largest amount of use in our country, bringing huge economic benefits to our country. But on the other hand, it also brings huge harm to food safety and ecological system. According to several organophosphorus poisoning incidents reported recently...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30
CPCG01N27/26G01N27/308
Inventor 赵波邵科峰周斌华张帆
Owner NANJING NORMAL UNIVERSITY
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