Preparation method of panaxatriol saponin

A technology of alcohol saponins and ginseng, which is applied in the field of preparation of panaxatriol saponins, can solve the problems of difficult reuse of silica gel, long production cycle and high production cost, and achieves the effects of being suitable for industrialized production, reducing production cost and long production cycle

Active Publication Date: 2017-08-04
YUNNAN YUNKE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In these literatures, most of the adsorption resins used are broad-spectrum styrene nonpolar macroporous adsorption resins with large pore diameter and low selectivity. The average pore diameter of this type of resin is about 120 μm, and the desugar function is strong. There is not much difference in chemical structure, and the separation effect of ginseng triol saponin and panaxadiol saponin with a molecular weight difference of about 200 is poor even if gradient elution is used. Triol saponin is often entrained in diol saponin, and diol saponin is often entrained in triol saponin , the obtained ginseng triol saponin product content can only reach 60-70% content, can not reach better effect of separation and removal of ginseng triol saponin; fine separation and prepar

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the preparation method of panaxatriol saponin is as follows:

[0024] (1) After the ginseng was crushed to 60 mesh, an ethanol solution with a concentration of 60% of the mass percentage of 7 times the mass of the crude drug was added to the raw material, cold soaked and extracted at room temperature for 12 hours, and after filtration, the residue of the crude drug was added with a concentration of 5 times the mass percentage of the crude drug at a concentration of 60 % ethanol solution for extraction of 12h, after filtering, adding crude drug quality 3 times the mass percentage concentration of 60% ethanol solution for extraction of 12h in the filter residue;

[0025] Combine the extracts for 3 times and concentrate under reduced pressure to recover the solvent, dilute the concentrated solution with purified water to 5 times the crude drug amount, filter it with diatomaceous earth, and collect the filtrate;

[0026] (2) Pass the filtrate of step (1) throu...

Embodiment 2

[0031] Embodiment 2: the preparation method of panaxatriol saponin is as follows:

[0032] (1) After the American ginseng was crushed to 60 mesh, an ethanol solution with a concentration of 8 times the mass percentage of the crude drug was added to the raw material and extracted by cold soaking at room temperature for 12 hours. % ethanol solution for extraction of 12h, after filtering, add crude drug quality 4 times the mass percentage concentration of 70% ethanol solution for extraction of 12h in the filter residue;

[0033] Combine the extracts for 3 times and concentrate under reduced pressure to recover the solvent, dilute the concentrated solution with purified water to 4 times the crude drug amount, filter it with diatomaceous earth, and collect the filtrate;

[0034] (2) Pass the filtrate of step (1) through a macroporous adsorption resin (IIZ-803) column with a pore size of 50-65 μm to absorb ginsengtriol saponin, wash with water until the effluent is colorless after l...

Embodiment 3

[0039] Embodiment 3: the preparation method of panaxatriol saponin is as follows:

[0040] (1) After taking Sanqi as the raw material and crushing it to 60 mesh, add an ethanol solution with a concentration of 75% of the mass percentage of the crude drug to 7.5 times the mass percentage of the raw material, cold soak and extract at room temperature for 12 hours, and add a concentration of 5 times the mass percentage of the crude drug mass to the filter residue after filtration 75% ethanol solution for extraction for 12 hours, and after filtering, add crude drug quality 3 times the mass percentage concentration of 75% ethanol solution for extraction for 12 hours;

[0041] Combine the extracts for 3 times and concentrate under reduced pressure to recover the solvent, dilute the concentrated solution with purified water to 5 times the crude drug amount, filter it with diatomaceous earth, and collect the filtrate;

[0042] (2) Pass the filtrate of step (1) through a 65-70 μm pore ...

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Abstract

The invention discloses a preparation method of panaxatriol saponin. The method comprises the steps of performing low alcohol extraction and concentration and kieselguhr filtration on panax notoginseng, ginseng or American ginseng as raw materials, performing selective adsorption on the panaxatriol saponin by using macroporous resin with an aperture of 50-70 micrometers, resolving with an alcohol solution to form a crude product of the panaxatriol saponin, performing methanol, alcohol, methanol water alcohol water dissolution on the crude product of the panaxatriol saponin, adding acetonitrile or ethyl acetate for precipitation, discarding a precipitate, diluting a supernate with water to form a crude product solution of the panaxatriol saponin, performing chromatographic resin column and alcohol solution resolution and separation on the crude product solution of the panaxatriol saponin to form a panaxatriol saponin solution, removing flavonoid pigment impurities by polyamide or anion exchange resin, decoloring and drying the panaxatriol saponin solution with active carbon at last to form the high-purity panaxatriol saponin. A content of the panaxatriol saponin is greater than 95%. The method hardly uses an organic solvent, and is lower in production cost, simple and easy to operate and suitable for industrial production.

Description

technical field [0001] The invention relates to a preparation method of panaxatriol saponin, which belongs to the field of preparation of plant extracts. Background technique [0002] Panax notoginseng, ginseng, and American ginseng are precious Chinese medicinal materials in my country. The main components are ginsenosides. Body saponins can be divided into 20(S)-protopanaxadiol (panaxadiol Saponin, PDS) and 20(S)-protopanaxatriol Saponin, PTS) according to their structural characteristics. Protopanaxadiol saponins in Panax notoginseng, ginseng and American ginseng are mainly composed of ginsenosides Rb1 and Rd, protopanaxatriol saponins are mainly composed of ginsenosides Re and Rg1, and Panax notoginseng also includes notoginsenoside R1. [0003] Panaxatriol saponins with Rg1 as the main component can inhibit platelet aggregation, reduce cerebrovascular resistance, improve microcirculation and brain dysfunction, and reduce blood viscosity; its mechanism of action is to in...

Claims

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Application Information

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IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 张文生曾立品罗金明辛文锋蔡群虎刘石磊胡会泽杨雪王芬
Owner YUNNAN YUNKE PHARMA
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