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Dendrobium-devonianum-paxt-dried-stem polysaccharide and preparing method and application thereof

A technology of Dendrobium purpurea and polysaccharide, applied in the field of medicinal chemistry, can solve the problems of unclear active ingredients and structural characteristics of Dendrobium polysaccharide, high cost of raw materials, slow growth, etc. Effect

Active Publication Date: 2017-08-11
赵静
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The 2015 edition of "Chinese Pharmacopoeia" contains Dendrobium officinale and Dendrobium (Dendrobium nobile, Dendrobium chrysotoxum, Dendrobium fimbriatum, etc.), among which Dendrobium officinale has the highest efficacy, but its growth conditions are harsh and natural. Low reproductive capacity, slow growth, expensive
However, the raw material it uses is Dendrobium officinale, and its raw material cost is high, and the specific active ingredients and structural characteristics of the obtained Dendrobium polysaccharide are also not specified in the application, which further limits its application
Although in the above-mentioned published documents, Dendrobium polysaccharides were all prepared, most of them were carried out for Dendrobium candidum with high raw material cost, and no research on the relationship between structural feature analysis and activity was carried out, so there were problems that could not be further studied or applied. Market problems have seriously affected the application of Dendrobium polysaccharides as potential active agents in the fields of medicine, food or health products

Method used

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  • Dendrobium-devonianum-paxt-dried-stem polysaccharide and preparing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Preparation of Dendrobium purple polysaccharide:

[0046] (1) Dry and powder the stems of Dendrobium officinale, degrease with 80% ethanol under reflux, add water to make the ratio of solid to liquid 1:30, extract by microwave reaction, microwave power 800w, reaction time 9min.

[0047] (2) Concentrate the water extract to a certain volume, add 4 times the volume of 95% ethanol to precipitate, let stand for 30 minutes, centrifuge at 4000g for 10 minutes, retain the precipitate, add water to redissolve, freeze-dry to obtain crude polysaccharide, and the extraction rate is 28.3%. Wherein, the freeze-drying conditions are: temperature -50--70°C, absolute pressure about 4-7 Pa.

[0048] (3) Add the above crude polysaccharide and deionized water to form a solution with a concentration of 5-10mg / mL, perform ultrafiltration (molecular weight cut-off: >3kDa, Millipore, Billerica, MA, USA), discard the ultrafiltrate, and rehydrate repeatedly , ultrafiltration, adopting phenol-s...

Embodiment 2

[0050] Preparation of Dendrobium purple polysaccharide:

[0051] Properly crushed fresh or dried Dendrobium purple-skinned dendrobium is added to water for extraction, and the water extract is treated with an ultrafiltration membrane with a molecular weight cut-off of 10,000 Da; after the filtrate is discarded, water replenishment and ultrafiltration are repeated several times, and the phenol-sulfuric acid method is used to track and detect sugar content, until basically no sugar is detected in the filtrate, and the retentate is freeze-dried to obtain the purified Dendrobium polysaccharide (DDP), which is detected by iodine-potassium iodide without starch, and without uronic acid by m-hydroxybiphenyl colorimetry Check out.

Embodiment 3

[0053] Identification of polysaccharides from Dendrobium nobile:

[0054] (1) Monosaccharide composition analysis of polysaccharides from Dendrobium nobile

[0055] Gas chromatography-mass spectrometry was used to determine the monosaccharide composition of Dendrobium dendrobii polysaccharides. Take 5 mg of dendrobium dendrobium polysaccharide samples and add 1mL 2M TFA to hydrolyze them under microwave. The microwave conditions are: power 500W, microwave time 4min, after cooling, hydrolyze The solution was blown dry with nitrogen, added 0.5mL pyridine (containing 20mg hydroxylamine hydrochloride) and reacted at 90°C for 30min, then added 0.5mL acetic anhydride and heated at 90°C for 30min, blown dry with nitrogen, dissolved in 1mL methanol and determined by GC-MS.

[0056] The GC-MS analysis conditions were: Agilent 19091S-433 column, HP-5MS capillary column (30m×0.25mm×0.25μm); the inlet temperature was 250°C; the carrier gas was He gas, and the column flow rate was 1.0mL / mi...

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Abstract

The invention relates to a dendrobium-devonianum-paxt-dried-stem polysaccharide with the effects of immunological enhancement and the hypoglycemic activity and a preparing method. The preparing method of the dendrobium-devonianum-paxt-dried-stem polysaccharide includes the steps of water extraction, alcohol precipitation, redissolving, drying or water extraction, ultra-filtration, drying and the like; the dendrobium-devonianum-paxt-dried-stem polysaccharide is characterized in that development does not occur when an iodine-potassium iodide reagent is added, the molecular weight is 3*10<5> Da-5*10<5> Da, a monosaccharide composition mainly comprises mannose, Acetyl-Man an a small amount of glucose (Glc), and the composition ratio of the mannose to the Glc is larger than 20:1; a glycosidic bond is mainly beta-1,4-Manp and beta-1,4-Glcp. The dendrobium-devonianum-paxt-dried-stem polysaccharide has the effects of immunological enhancement and the hypoglycemic activity, and can be used for developing relative products for treating immunosuppression and hyperglycemia.

Description

technical field [0001] The invention belongs to the field of medicinal chemistry, and in particular relates to a dendrobium purple polysaccharide and a preparation method thereof, in particular to the application of the dendrobium purple polysaccharide in the aspects of immune regulation and hypoglycemic activity. Background technique [0002] Dendrobium dendrobium is the dry stem of Dendrobium devonianum Paxt., a dicotyledonous plant medicine orchid family. It has the effects of nourishing stomach and promoting body fluid, nourishing yin and clearing heat. Dendrobium purple-skinned Dendrobium has jointed stems, slightly fleshy, leathery leaves, and alternates. Because the surface of the stems is mostly purple after removing the leaf sheath film in the autumn and winter harvest season, the purple color is more obvious when it grows in places with strong sunlight, so it is commonly known as Dendrobium purple-skinned Dendrobium or Purple Piran. The stalks of fresh Dendrobium ...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61K31/715A61P37/02A61P3/10A23L33/125
CPCA23V2002/00A61K31/715C08B37/0003C08B37/006A23V2250/21A23V2200/328A23V2200/324
Inventor 赵静李绍平邓勇
Owner 赵静
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