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Preparation method of folic acid-modified molybdenum disulfide-coated periodic mesoporous organic silicone nano drug-loaded compound

A mesoporous organic silicon and molybdenum disulfide technology, which is applied in the direction of non-active ingredients of polymer compounds, drug combination, drug delivery, etc., can solve the problems of short drug action time, poor bioselectivity, and low utilization rate, and the method is simple , High release rate, high loading effect

Inactive Publication Date: 2017-11-24
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to its advantages of easy surface targeted modification, long circulation and residence time, easy penetration into cells, slow release and controlled release, etc., the nano drug delivery system can overcome the poor bioselectivity and utilization of existing small molecule drug preparations. Low, poor stability, short drug action time, serious adverse reactions and other defects

Method used

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  • Preparation method of folic acid-modified molybdenum disulfide-coated periodic mesoporous organic silicone nano drug-loaded compound
  • Preparation method of folic acid-modified molybdenum disulfide-coated periodic mesoporous organic silicone nano drug-loaded compound
  • Preparation method of folic acid-modified molybdenum disulfide-coated periodic mesoporous organic silicone nano drug-loaded compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Disperse 2.0g CTAB in a mixed solvent of 20mL deionized water and 50mL ethanol, heat to 35°C, and stir to dissolve. Then 1 mL of ammonia water was added and stirred for 1 hour. Then add 1mL TEOS, 0.25mL TESPTS, continue to react for 1 hour, and centrifuge at 10000rpm to obtain precipitates which are silica nanoparticles. The precipitate was refluxed in a mixed solution of hydrochloric acid / methanol for 6 hours, and then centrifuged to obtain a precipitate. The above reflux operation was repeated three times, centrifuged and freeze-dried to obtain periodic mesoporous organic silicon nanoparticles PMOs.

[0039] (2) Accurately weigh and disperse 15 mg of PMOs in 40 mL of ethanol, add 0.15 mL of (3-aminopropyl) dimethylethoxysilane MPTMS, reflux in an oil bath at 50 ° C for 5 h, and collect by centrifugation to obtain PMOs-SH.

[0040] (3) Disperse 10 mg of PMOs-SH in 10 mL of PBS solution with a pH of 7.4. While stirring, add 5 mL of DOX (5 mg) in PBS solution dropwi...

Embodiment 2

[0045] (1) Disperse 2.0g CTAB in a mixed solvent of 40mL deionized water and 10mL ethanol, heat to 45°C, and stir to dissolve. Then 1 mL of ammonia water was added and stirred for 1 hour. Then add 1mL TEOS, 0.4mL TESPTS, continue to react for 1 hour, and centrifuge at 10000rpm to obtain precipitates that are silica nanoparticles. The precipitate was refluxed in a mixed solution of hydrochloric acid / methanol for 6 hours, and then centrifuged to obtain a precipitate. The above reflux operation was repeated three times, centrifuged and freeze-dried to obtain periodic mesoporous organic silicon nanoparticles PMOs.

[0046] (2) Accurately weigh and disperse 15 mg of PMOs in 40 mL of ethanol, add 0.3 mL of (3-aminopropyl) dimethylethoxysilane MPTMS, reflux in an oil bath at 50 °C for 5 h, and collect by centrifugation to obtain PMOs-SH.

[0047] (3) Disperse 10 mg of PMOs-SH in 20 mL of PBS solution with a pH of 7.4. While stirring, add 10 mL of DOX (5 mg) in PBS solution dropwise,...

Embodiment 3

[0052] (1) Disperse 4.0g CTAB in a mixed solvent of 40mL deionized water and 10mL ethanol, heat to 55°C, and stir to dissolve. Then 2 mL of ammonia water was added and stirred for 1 hour. Then add 1mL TEOS, 0.15mL TESPTS, continue to react for 1 hour, and centrifuge at 10000rpm to obtain precipitates which are silica nanoparticles. The precipitate was refluxed in the mixed solution of hydrochloric acid / methanol for 6 hours, and then centrifuged to obtain the precipitate. The reflux operation was repeated three times, centrifuged and freeze-dried to obtain periodic mesoporous organic silicon nanoparticles PMOs.

[0053] (2) Accurately weigh and disperse 15 mg of PMOs in 40 mL of ethanol, add 0.4 mL of (3-aminopropyl) dimethylethoxysilane MPTMS, reflux in an oil bath at 50 ° C for 5 h, and collect by centrifugation to obtain PMOs-SH.

[0054] (3) Disperse 10 mg of PMOs-SH in 20 mL of PBS solution with a pH of 7.4. While stirring, add 10 mL of DOX (5 mg) in PBS solution dropwise...

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Abstract

The invention relates to a preparation method of a folic acid-modified molybdenum disulfide-coated periodic mesoporous organic silicone nano drug-loaded compound. The preparation method comprises the following steps: dispersing cetyltrimethylammonium bromide in a solvent; adding TEOS and TESPTS into the mixed liquid for reacting to obtain PMOs; modifying the surface of the PMOs with sulfydryl to obtain PMOs-SH; mixing the obtained product with doxorubicin DOX to obtain PMOs-DOX; preparing LA-PEI and modifying to molybdenum disulfide with the LA-PEI to obtain MoS2-LA-PEI; mixing the obtained product with PMOs-DOX, and performing ultrasonic treatment; then reacting the product with folic acid-modified bovine serum albumin BSA to obtain a product PMOs-DOX@MoS2-PEI-BSA-FA. The method provided by the invention is simple; the product realizes dual-response conveying for pH and illumination, has relatively high biocompatibility and is expected to be applied to tumor targeting and synergistic treatment.

Description

technical field [0001] The invention belongs to the field of targeting nanomaterials, and in particular relates to a preparation method of a folic acid-modified molybdenum disulfide-wrapped periodic mesoporous organosilicon nanometer drug-loaded compound. Background technique [0002] Malignant tumors seriously threaten human health and life. In recent years, the morbidity and mortality of tumors have been increasing. Cancer treatment has become a major challenge in the current medical research field. At present, the treatment methods for tumors are mainly surgical resection, radiotherapy, and chemical drug therapy, and some tumors also use gene and biological therapy as adjuvant therapy. As a means of systemic treatment, chemotherapy drugs can effectively fight against the invasion and metastasis of tumor cells, so chemotherapy plays a dominant role in the comprehensive treatment of tumors. However, traditional chemotherapeutic drugs still have certain limitations, such as...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K41/00A61K31/704A61K47/04A61K47/42A61K47/34A61K47/22A61P35/00
CPCA61K9/5169A61K9/0002A61K9/5123A61K31/704A61K41/0052A61K2300/00
Inventor 朱利民吴建荣
Owner DONGHUA UNIV
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