Bacillus coagulans as well as microbial preparation, preparation method and application thereof

A technology of bacillus coagulans and microbial preparations, applied in the field of environmental protection, can solve the problems of reducing the release of ammonia and hydrogen sulfide, achieve good deodorization effect, eliminate foul smell, and improve the environment

Active Publication Date: 2017-11-28
上海道多生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The present invention aims at the problem that the malodorous gas treatment agent in the prior art is difficult to effectively inhibit the growth of harmful bacteria for a long time under complex (high temperature, high osmotic pressure) conditions, and provides a highly efficient deodorizing microbial preparation suitable for complex and harsh environments. It can efficiently decompose odorous and harmful substances in a high temperature and high osmotic pressure environment, reduce the release of ammonia and hydrogen sulfide, and eliminate odors for a long time

Method used

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  • Bacillus coagulans as well as microbial preparation, preparation method and application thereof
  • Bacillus coagulans as well as microbial preparation, preparation method and application thereof
  • Bacillus coagulans as well as microbial preparation, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Screening and Identification of Bacillus coagulans Daoduo 4

[0038] Formulated with CaCO 3 For the MRS solid plate of the emulsion, collect microbial samples from the wet soil, streak the sample on the above plate at 50°C, select the colony containing the calcium-dissolving circle, and repeatedly streak on the above plate to obtain a single strain .

[0039] Among them, MRS medium formula (1L): peptone 10g, beef extract 10g, yeast extract 5g, K 2 HPO 4 2g, triammonium citrate 2g, sodium acetate 5g, glucose 20g, Tween 80 1mL, MgSO 4 ·7H 2 O 0.5g, MnSO 4 4H 2 O 0.25g.

[0040] Send one of the screened strains to the Institute of Microbiology, Chinese Academy of Sciences for strain identification. According to the comprehensive analysis of the strain’s cell morphology, physiological and biochemical experiments, 16S rRNA gene sequence and other experimental data, refer to "Bergey's Handbook of Systematic Bacteriology" and International Journal Of Systema...

Embodiment 2

[0050] Embodiment 2 Deodorizing microorganism preparation and preparation method thereof

[0051] A deodorizing microbial preparation containing Bacillus coagulans Daoduo 4, which is fermented sour, with a pH value of 4 to 5, and the total number of effective viable bacteria ≥ 1.0×10 9 CFU / mL, of which, the main effective live bacteria Bacillus coagulans Daoduo 4 is 10 9 ~10 11 CFU / mL.

[0052] The preparation method of deodorizing microbial preparation is as follows:

[0053] (1) plate culture, using solid activation medium, plate activation of Bacillus coagulans Daoduo 4

[0054] Inoculate Bacillus coagulans Daoduo 4 on a solid activated medium plate by streak inoculation method, culture at 50°C for 36-48 hours, make it recover, and form a single colony.

[0055] Wherein the solid activation medium is prepared according to the following weight percentages: 1% peptone, 0.5% yeast powder, 1% sodium chloride, 2% agar, and the rest is distilled water, sterilized at 121°C for...

Embodiment 3

[0066] Embodiment 3 Utilize above-mentioned microbial agent to carry out deodorization project in a landfill in Suzhou

[0067] Use the above-mentioned microbial preparations to prepare 500 kg of deodorant liquid at a ratio of 1:50.

[0068] In this example, gas samples were collected from the working surface of the domestic waste landfill, and the odor concentration and volatile organic compounds (VOC) were used as indicators to comprehensively reflect changes in environmental odor.

[0069] Odor concentration is an indicator that reflects people's subjective perception of odor, and it is measured by the three-point comparative stinky bag method, which is dimensionless. Ammonia is measured by solution absorption-spectrophotometry, VOC is measured by gas chromatography, and the units are mg / m 3 , from a quantitative point of view to reflect the change of the odor caused by the ambient gas.

[0070] The analysis methods for the content of volatile organic compounds (VOC) in s...

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Abstract

The invention discloses bacillus coagulans Daoduo 4 as well as a screening method thereof and further discloses a microbial preparation containing bacillus coagulans Daoduo 4 as well as a preparation method and an application of the microbial preparation. The microbial preparation is simple to culture, stable in structure, non-toxic, harmless, free of side effects and capable of effectively inhibiting breeding and growth of harmful microorganisms such as putrefying bacteria, pathogenic bacteria and the like, has relatively high stress resistance to the environment, has the advantages of high temperature resistance, acid resistance, choline resistance and the like and can inhibit growth of harmful bacteria in garbage, feces and sewage under complicated conditions, remove odor, reduce pollution and improve the environment. Besides, the bacillus coagulans can effectively remove VOCs (volatile organic compounds) and remarkably reduce harm of decoration pollution to human health after being applied to newly decorated houses and other places.

Description

[0001] Cross References to Related Applications [0002] This application is based on and claims priority from a prior Chinese patent application with application number 2017101046947 filed on February 24, 2017, the entire contents of which are hereby incorporated by reference. technical field [0003] The invention relates to the technical field of environmental protection, in particular to a bacillus coagulans, a screening method thereof, a microbial preparation containing the bacillus coagulans, a preparation method and an application of the microbial preparation. Background technique [0004] With the rapid development of urbanization in our country, the level of urbanization has steadily increased, and the population has continued to increase, the environmental pollution caused by municipal solid waste has attracted people's attention. Domestic garbage accumulated at garbage collection points and garbage transfer stations causes problems such as odor emission, breeding...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02B01D53/84B01D53/58B01D53/52C12R1/07
CPCB01D53/52B01D53/58B01D53/84B01D2257/708C12N1/02C12N1/20C12N1/205C12R2001/07Y02A50/20
Inventor 唐鸿志许平杨科武
Owner 上海道多生物科技有限公司
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