Application of Sporosarcina koreensis CGMCC No. 5915, preparation method of lipopeptide surfactins and composition of Sporosarcina koreensis

A sporococcus spore and surfactin technology, applied in the field of microorganisms, can solve the problems of limited surfactin application, low surfactin yield and high production cost, and achieve the effects of maintaining soil moisture, uniform fertilizer, and uniform dispersion

Inactive Publication Date: 2018-03-23
DONGGUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But because the yield of surfactant is very low and the production cost is very high, these limit the production of surfactant and its application in industrial production.

Method used

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  • Application of Sporosarcina koreensis CGMCC No. 5915, preparation method of lipopeptide surfactins and composition of Sporosarcina koreensis
  • Application of Sporosarcina koreensis CGMCC No. 5915, preparation method of lipopeptide surfactins and composition of Sporosarcina koreensis
  • Application of Sporosarcina koreensis CGMCC No. 5915, preparation method of lipopeptide surfactins and composition of Sporosarcina koreensis

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Experimental program
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Embodiment approach

[0045] According to a specific embodiment of the present invention, the pesticide is chlorothalonil.

[0046] 3) Application of Sporosarcina koreansis with preservation number CGMCC No.5915 in antibacterial, antiviral and agricultural pest inhibition.

[0047] Wherein, the bacteria may be various bacteria, for example, but not limited to Escherichia coli, Staphylococcus aureus, Streptococcus thermophilus, Pseudomonas aeruginosa, Aspergillus niger and the like. And according to its non-toxic properties, the Korean bacillus sarcini (Sporosarcina koreansis) with the preservation number CGMCC No.5915 can also be applied in food as a preservative.

[0048] The virus may be, but not limited to, TMEV (Turrell Murine Encephalomyelitis Virus), HSV-1 (Herpes Simplex Virus-1) and HSV-2 (Herpes Simplex Virus-2), HIV and the like.

[0049] The agricultural pests may be rice planthopper, powdery mildew, corn borer, cotton bollworm, wheat rust, cotton aphid, rice sheath blight, rice blast, ...

Embodiment 1

[0075] Preparation method and identification of lipopeptide surfactin produced by Sporosarcina koreensis

[0076] (1) Preparation of Surfactin

[0077] A single colony of Sporosarcina koreensis was dissolved in a fermentation medium (LB liquid medium: 5 g of yeast extract powder, 10 g of tryptone, and 10 g of sodium chloride) at 37° C., added with distilled water and stirred to dissolve, and mixed with 5 mol / L Adjust the pH to about 7.2 with NaOH, adjust the volume to 1000ml with distilled water, sterilize at 121°C for 20min) and ferment and cultivate for 48 hours to obtain the fermentation broth.

[0078] The fermentation broth was centrifuged at 10000rpm for 25min to remove the precipitate and collect the supernatant. Then add hydrochloric acid with a concentration of 6 mol / L to the collected supernatant, stir with a glass rod to make it evenly mixed, and adjust the pH of the supernatant to about 2.0. Then put it in a refrigerator at 4°C overnight, centrifuge at 10,000 rpm...

Embodiment 2

[0091] This example is used to illustrate the analysis of the surface activity of the oil drainage circle of the fermentation broth

[0092] Add 1 g of Sudan Red III to 300 mL of liquid paraffin, stir and mix evenly with a glass rod, and filter under reduced pressure with a vacuum pump to remove insoluble impurities. Take 25mL of liquid paraffin stained with Sudan Red III and pour it into a petri dish with a diameter of about 9cm. After the whole petri dish is covered with liquid paraffin, slowly add 1 mL of Surfactin (2%) produced by Sporosarcina koreansis, 1 mL of 0.1% sodium lauryl sulfate solution, and 1 mL of standard Surfactin (Surfactin, brand name Sigma) at a concentration of 0.5 mg / mL -Aldrich, product number S3523) solution, measure the diameter of the oil drainage ring with a ruler and take a picture to record it, add 1mL of blank methanol to the center of the petri dish as a blank control, and repeat the experiment of each material oil drainage ring three times, th...

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Abstract

The invention relates to the field of microbes, in particular to application of Sporosarcina koreensis CGMCC No. 5915, a preparation method of lipopeptide surfactins and a composition of Sporosarcinakoreensis. The technical scheme provided herein exhibits good effects in all the agriculture field, medicine field, cosmetics field, washing supplies field, food field and environmental protection field.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to the application of Bacillus sarcina Koreana CGMCC No.5915 in producing lipopeptide surfactant and a preparation method of lipopeptide surfactant. Background technique [0002] Some bacillus can produce a kind of biological surfactant——surfactin (surfactin) in the process of growth, reproduction and metabolism. Compared with synthetic chemical surfactants, quasi-surfactants are not only less toxic to the ecosystem, but also biodegradable. Therefore, it has great potential in food, medicine, agriculture, cosmetics and many other fields. However, because the yield of surfactant is very low and the production cost is very high, these limit the industrial production and application of surfactin in industrial production. Contents of the invention [0003] The purpose of the present invention is to overcome the above defects in the prior art, to provide the application of a Korean baci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/00B01F17/30A01N63/02A01P1/00A01P3/00C11D1/36A62D3/02C09K17/14A61K8/99A61K8/64A61Q19/00C12R1/01A62D101/04C09K23/30
CPCA61Q19/00A62D3/02A61K8/64A61K8/99C09K17/14C11D1/36C12P21/00A61K2800/85A62D2101/04A01N63/10C09K23/00
Inventor 王世伟李萌孙艳梅刘伟杰陈慧海黎永青
Owner DONGGUAN UNIV OF TECH
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