New strategy for realizing one-pot-method chromogenic detection of glucose

Abstract

The invention relates to the fields of nano materials, catalysis and analytical chemistry, and particularly comprises a new strategy for realizing the one-pot-method chromogenic detection of glucose.According to the method, the characteristic of the nano structure of bovine serum albumin and the property of a functional group of the bovine serum albumin are utilized; the bovine serum albumin is mixed with a divalent cobalt ion; a reaction is carried out at a room temperature after sodium borohydride is added to generate a cobaltosic oxide nano sphere under the stabilization action of the bovine serum albumin. According to the method, a biological material is used as a template; the preparation process is simple; the reaction condition is mild and environment-friendly; the nano structure is easily controlled. The material has the advantage of having mimic enzyme catalysis characteristic and magnetism and a surface-modifiable functional group at the same time; glucose oxidase can be quickly modified based on the advantages and can be used for the chromogenic detection of the recoverable glucose. The new strategy has another important advantage that the active pH (potential of Hydrogen) range of the glucose oxidase is changed by utilizing the fixation, on the surface of the cobaltosic oxide nano sphere, of the glucose oxidase; thus, the one-pot-method chromogenic analysis is realized and a nano material, to which the new strategy provided by the patent relates, has wide application prospects in the fields of the analytical chemistry, environmental engineering and the catalysis.

Description

technical field [0001] The invention relates to the fields of nanomaterials, catalysis and analytical chemistry, and specifically includes a new strategy for realizing one-pot color detection of grapes. Background technique [0002] In recent years, cobalt tetroxide, as a metal oxide with important industrial applications, has attracted extensive attention in various fields. Researchers have synthesized nano-scale cobalt tetroxide using various methods. With its unique structure and properties, it is widely used in batteries, capacitors, immunoassays, catalysis and other fields. Wei et al. in Microwave-Assisted Synthesis of Mesoporous Co. 3 O 4 In Nanoflakes for Applications in LithiumIon Batteries and Oxygen Evolution Reactions", porous Co was synthesized by microwave hydrothermal and low temperature conversion methods. 3 O 4 nanosheets and use them in batteries ( ACS Applied Materials & Interfaces 2015, 7, 3306-3313). Rao et al in "Ultralayered Co 3 O 4 In the ar...

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Problems solved by technology

These reports require two-step detection of glucose, the process is more complicated

[0004] To sum up, in the existing reports, glucose oxidase needs to be added when detecting glucose, and the detection of glucose is realized by a two-step method. Enzymes and materials cannot be recycled

In order to realize the one-pot method detection, people usually change the active pH range of nanomaterials with imitation peroxidase activity to realize the one-pot method (Han, L. et al. , Au@Ag Heterogeneous Nanorodsas Nanozyme Interfaces with Peroxidase-Like Activity and Their Application for One-Pot Analysis of Glucose at Nearly Neutral pH. ACS Applied Materials & Interfaces 2015, 7 (26), 14463-14470), while ignoring the attempt to change the active pH range of glucose oxidase, thereby increasing the limitation on nanomaterials, because it is difficult to change the active pH range of nanomaterials

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