Microcapsule for preventing and treating plant diseases as well as preparation method and application of microcapsule

A microcapsule, plant disease technology, applied in the directions of botanical equipment and methods, chemicals for biological control, plant growth regulators, etc., can solve problems such as the absence of Pseudomonas fluorescens

Active Publication Date: 2018-04-06
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current dosage form of Pseudomonas fluorescens is mainly wettable powder, and there is no report related to the microcapsule preparation of Pseudomonas fluorescens

Method used

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  • Microcapsule for preventing and treating plant diseases as well as preparation method and application of microcapsule
  • Microcapsule for preventing and treating plant diseases as well as preparation method and application of microcapsule
  • Microcapsule for preventing and treating plant diseases as well as preparation method and application of microcapsule

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～5

[0034] The preparation of embodiment 1~5 Pseudomonas fluorescens microcapsules

[0035] 1. Using liquid fermentation method to cultivate Pseudomonas fluorescens fermentation broth

[0036] 1.1 Optimization of fermentation medium

[0037] Culture material and culture condition

[0038] The strain used: Pseudomonas fluorescens SN15-2 (isolated from plant rhizosphere soil, can be purchased from the State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology).

[0039] Initial fermentation medium: glycerol 1.0%, peptone 2.0%, magnesium sulfate heptahydrate 0.15%, dipotassium hydrogen phosphate 0.15%, pH 7.0.

[0040] Alternative carbon sources: glucose, soluble starch, maltose, sucrose, corn flour, brown sugar, lactose, bran, glutinous rice flour, sorghum flour, wheat flour.

[0041] Replacement nitrogen source: yeast extract, yeast powder, peanut powder, bean cake powder, soybean powder, casein, urea, ammonium sulfate, potassium nitrate.

...

Embodiment 6

[0069] Example 6 Field fusarium wilt control test of Pseudomonas fluorescens microcapsules

[0070] (1) Test method

[0071] In the field soil of watermelon fusarium wilt morbidity symptom, divide three district tests, three districts carry out field fusarium wilt control test to the Pseudomonas fluorescens microcapsules prepared by embodiment 2,4,5 respectively, each district 5 groups of parallel tests, They are:

[0072] Control group 1 (blank control): no treatment with any drug;

[0073] Control group 2 (Azoxystrobin): treated with Azoxystrobin;

[0074] Experimental group 1: treated with 100 million CFU / g Pseudomonas fluorescens microcapsules 300 times liquid;

[0075] Experimental group 2: treated with 500 million CFU / g Pseudomonas fluorescens microcapsules at 300 times;

[0076] Experimental group 3: treated with 1 billion CFU / g Pseudomonas fluorescens microcapsules 300 times liquid;

[0077] The above-mentioned treatment areas of each group used a measuring cup to...

Embodiment 5

[0089] The preventive effect of table 3 embodiment 5 Pseudomonas fluorescens microcapsules on watermelon wilt

[0090]

[0091] The preventive effect of table 4 embodiment 4 Pseudomonas fluorescens microcapsules on watermelon wilt

[0092]

[0093] The preventive effect of table 5 embodiment 2 pseudomonas fluorescens microcapsules on watermelon wilt

[0094]

[0095] Test result shows, use the Pseudomonas fluorescens microcapsules 500mL of embodiment of the present invention 2,4,5 to watermelon irrigation root after 20 days, the watermelon plant blight disease incidence rate all reduces than before spraying, and three groups implement The incidence rate of each treatment in the case was significantly lower than that of the blank control. Wherein the 1,000,000,000 CFU / g Pseudomonas fluorescens microcapsules in Example 5 have the highest preventive effect, reaching 69.56%, which is equivalent to the chemical pesticide azoxystrobin. Fusarium wilt of watermelon also sho...

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Abstract

The invention discloses a microcapsule for preventing and treating plant diseases. The microcapsule contains pseudomonas fluorescens, a protective agent and a carrier; and the protective agent is selected from one or two or more of maltodextrin, sodium caseinate and sodium carboxymethyl cellulose. The invention further discloses a preparation method and application of the microcapsule. The microcapsule containing the pseudomonas fluorescens is formed by using liquid fermentation to produce the pseudomonas fluorescens serving as an effective component and organically compounding the carrier, the protective agent and the pseudomonas fluorescens. The microcapsule disclosed by the invention can be used for preventing and treating various plant soil-borne diseases and particularly has a bettereffect for preventing and treating vegetable bacterial wilt and plant wilt; in addition, compared with a living bacterial preparation produced by using a traditional method, the microcapsule has stronger vitality and stress tolerance as well as more effects and functions and is more suitable for production application. The problems that large-scale production cannot be realized and dust is generated in production and application processes due to short shelf life and high death rate during processing of a current biopesticide containing the pseudomonas fluorescens are solved.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to a microcapsule for preventing and controlling plant diseases, a preparation method and application thereof. Background technique [0002] Bacterial wilt is a worldwide devastating soil-borne bacterial disease caused by Ralstonia solanacearum (abbreviated as Ralstonia solanacearum). It is prone to outbreaks under high temperature and high humidity conditions and is widely distributed in tropical, Ralstonia solanacearum is also found in subtropical and temperate regions, and even in some low-temperature regions, which poses a great threat to global crop production. People have researched several kinds of chemical pesticides and biopesticides that can prevent and treat bacterial wilt, and combined with traditional control methods, they have a certain effect on the prevention and treatment of bacterial wilt, but there is still no significant e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/28A01N63/02A01P1/00A01P3/00
CPCA01N25/28A01N63/10
Inventor 王伟王晓冰陈俊
Owner EAST CHINA UNIV OF SCI & TECH
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