Microcapsule for preventing and treating plant diseases as well as preparation method and application of microcapsule
A microcapsule, plant disease technology, applied in the directions of botanical equipment and methods, chemicals for biological control, plant growth regulators, etc., can solve problems such as the absence of Pseudomonas fluorescens
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Embodiment 1~5
[0034] The preparation of embodiment 1~5 Pseudomonas fluorescens microcapsules
[0035] 1. Using liquid fermentation method to cultivate Pseudomonas fluorescens fermentation broth
[0036] 1.1 Optimization of fermentation medium
[0037] Culture material and culture condition
[0038] The strain used: Pseudomonas fluorescens SN15-2 (isolated from plant rhizosphere soil, can be purchased from the State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology).
[0039] Initial fermentation medium: glycerol 1.0%, peptone 2.0%, magnesium sulfate heptahydrate 0.15%, dipotassium hydrogen phosphate 0.15%, pH 7.0.
[0040] Alternative carbon sources: glucose, soluble starch, maltose, sucrose, corn flour, brown sugar, lactose, bran, glutinous rice flour, sorghum flour, wheat flour.
[0041] Replacement nitrogen source: yeast extract, yeast powder, peanut powder, bean cake powder, soybean powder, casein, urea, ammonium sulfate, potassium nitrate.
...
Embodiment 6
[0069] Example 6 Field fusarium wilt control test of Pseudomonas fluorescens microcapsules
[0070] (1) Test method
[0071] In the field soil of watermelon fusarium wilt morbidity symptom, divide three district tests, three districts carry out field fusarium wilt control test to the Pseudomonas fluorescens microcapsules prepared by embodiment 2,4,5 respectively, each district 5 groups of parallel tests, They are:
[0072] Control group 1 (blank control): no treatment with any drug;
[0073] Control group 2 (Azoxystrobin): treated with Azoxystrobin;
[0074] Experimental group 1: treated with 100 million CFU / g Pseudomonas fluorescens microcapsules 300 times liquid;
[0075] Experimental group 2: treated with 500 million CFU / g Pseudomonas fluorescens microcapsules at 300 times;
[0076] Experimental group 3: treated with 1 billion CFU / g Pseudomonas fluorescens microcapsules 300 times liquid;
[0077] The above-mentioned treatment areas of each group used a measuring cup to...
Embodiment 5
[0089] The preventive effect of table 3 embodiment 5 Pseudomonas fluorescens microcapsules on watermelon wilt
[0090]
[0091] The preventive effect of table 4 embodiment 4 Pseudomonas fluorescens microcapsules on watermelon wilt
[0092]
[0093] The preventive effect of table 5 embodiment 2 pseudomonas fluorescens microcapsules on watermelon wilt
[0094]
[0095] Test result shows, use the Pseudomonas fluorescens microcapsules 500mL of embodiment of the present invention 2,4,5 to watermelon irrigation root after 20 days, the watermelon plant blight disease incidence rate all reduces than before spraying, and three groups implement The incidence rate of each treatment in the case was significantly lower than that of the blank control. Wherein the 1,000,000,000 CFU / g Pseudomonas fluorescens microcapsules in Example 5 have the highest preventive effect, reaching 69.56%, which is equivalent to the chemical pesticide azoxystrobin. Fusarium wilt of watermelon also sho...
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