Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bionic oriented cartilage scaffold released by two-factor program and preparation method of bionic oriented cartilage scaffold

A two-factor, bionic technology, applied in the field of biomedicine, can solve the problems of transient, short action time, easy to be degraded, etc., and achieve the effects of good mechanical properties, simple preparation process and good biocompatibility.

Active Publication Date: 2018-06-15
FUZHOU UNIV
View PDF4 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, TGF-β1 and KGN have problems such as relatively short action time, easy to be degraded, and transient effects. If they can be controlled and released slowly, their biological effects can be better exerted

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bionic oriented cartilage scaffold released by two-factor program and preparation method of bionic oriented cartilage scaffold
  • Bionic oriented cartilage scaffold released by two-factor program and preparation method of bionic oriented cartilage scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041]1. Accurately weigh 200 mg of PLGA and dissolve in 4 mL of dichloromethane, ultrasonically oscillate to form an oil phase; then accurately weigh 10 mgKGN, and place in 1 mL of a mixture of ultrapure water and methanol (1:1, v / v ) at 37°C to completely dissolve it to form an inner aqueous phase. Slowly inject the obtained internal water phase into the obtained oil phase, and continue stirring and emulsifying for 10 min to make it uniformly dispersed to form a colostrum emulsion. The colostrum emulsion was dropped into 50 mL 1% PVA solution containing 5 mL 0.1% Tween-20 at 60 drops / min to form an external aqueous phase, and the double emulsion was formed by continuous stirring. The obtained double emulsion was fully centrifuged, the supernatant was discarded, and then repeatedly washed and centrifuged with ultrapure water for 3 times, and freeze-dried to obtain KGN-loaded PLGA microspheres, which were stored at -20°C in the dark.

[0042] 2. Dissolve PLL with a molecular ...

Embodiment 2

[0053] (1) Preparation of cartilage defect animal model of knee osteoarthritis: 20 male New Zealand rabbits were purchased and randomly divided into 4 groups according to body weight, with 5 rabbits in each group, namely: group A, model control group; group B, surface layer composite Material group; C group, transitional layer composite material group; D group, two-factor programmed release biomimetic oriented cartilage scaffold group. After one week of adaptive feeding, 0.3 mL / kg of Sumianxin II was injected intramuscularly, and an osteoarthritis model was established by cutting the anterior cruciate ligament. Intramuscular injection of penicillin sodium (150,000 U / cattle / day) was continued for one week after the operation, feeding continued for one month and moderate exercise was performed every day. One month later, the intramuscular injection of 0.3 mL / kg Sumianxin Ⅱ was continued for anesthesia, and then the epidermis and muscle tissue of the knee joint were cut longitudi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
quality scoreaaaaaaaaaa
Login to View More

Abstract

The invention discloses a bionic oriented cartilage scaffold released by a two-factor program and a preparation method of the bionic oriented cartilage scaffold. The bionic oriented cartilage scaffoldis formed by compounding an upper layer of materials and a lower layer of materials, wherein a composite material of an upper surface layer is prepared from collagen, chitosan, hyaluronic acid and KGN-loaded PLAG microspheres; the composite material of a lower transition layer is prepared from collagen, chitosan, silk fibroin and TGF-beta1-loaded polylysine-heparin sodium (PLL-HS) nanoparticles.The bionic orientated cartilage scaffold prepared in the invention integrates high biocompatibility and biodegradability of collagen, antibacterial property and biodegradability of chitosan, high mechanical properties of silk fibroin and lubrication and anti-inflammation effects of hyaluronic acid, has good proliferation and differentiation promoting effects on BMSCs, can relieve and treat KOA symptoms while repairing early and middle KOA cartilage defects, and is a relatively ideal medical material for repairing osteoarthritis cartilage defects.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a bionic oriented cartilage support with dual-factor programmed release and a preparation method thereof. Background technique [0002] Knee osteoarthritis (KOA) is an osteoarthritis disease characterized by inflammatory lesions such as deformation of articular cartilage, bone hyperplasia and necrosis, among which progressive degeneration of cartilage and remodeling of subchondral bone are the main The main clinical manifestations are persistent pain, swelling, mobility impairment, knee joint deformity, etc. in the knee joint, which seriously affect the health of patients. [0003] The clinical treatment of KOA is mainly divided into surgical treatment and non-surgical treatment. Surgical treatment mainly includes autologous or allogeneic osteochondral transplantation, joint debridement, drilling, osteotomy around the knee joint, knee arthrodesis, artificial total knee art...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/48A61L27/54A61L27/58
CPCA61L27/48A61L27/54A61L27/58A61L2300/236A61L2300/41A61L2300/412A61L2300/414A61L2300/622A61L2300/624A61L2430/06C08L89/00C08L5/08
Inventor 王建华张其清孙晓敏王营营黄晨光
Owner FUZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com