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Probiotics aloe juice and preparation method and applications thereof

The technology of aloe vera liquid and probiotics is applied in the field of gynecological medical treatment, which can solve the problems of causing diseases, poor efficacy of vaginal antibacterial drugs, imbalance of vaginal flora, etc. Effect

Inactive Publication Date: 2018-06-29
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides a probiotic aloe liquid, its preparation method and application, so as to solve the technical problem of poor curative effect of vaginal antibacterial drugs in the prior art
[0007] Another technical problem to be solved by the present invention is to realize that if the conventional aloe extract in the prior art is used as a vaginal external drug, it will easily lead to an imbalance of vaginal flora and cause diseases

Method used

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  • Probiotics aloe juice and preparation method and applications thereof
  • Probiotics aloe juice and preparation method and applications thereof
  • Probiotics aloe juice and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 (determination of probiotic aloe liquid fermentation conditions)

[0030] 1. Preparation of aloe vera juice: select fresh, plump, and fleshy Aloe vera leaves, remove leaves with lesions and soft rot, and the average weight of the leaves is about 300-400g. Wipe the surface of the leaves with alcohol to remove the epidermis, use a juicer to squeeze the mesophyll into aloe vera juice, subpackage, centrifuge at 6000rpm / min for 10min, and take the supernatant. The original aloe juice was sterilized by pasteurization, bathed in water at 55°C for 30 minutes, cooled and stored in a refrigerator at 4°C for future use.

[0031] 2. Fermentation: 6 kinds of probiotics after secondary activation were selected and mixed according to the ratio of 1:1 to prepare a compound probiotic suspension, which was added to the original aloe juice at a 5% inoculum amount, supplemented with glucose, and carried out co-fermentation.

[0032] 3. Setting of fermentation conditions: set ...

Embodiment 2

[0034] Embodiment 2 (bacteriostasis test)

[0035] Experimental materials: probiotics, typhoid fever, Salmonella enteritidis, Shiga flexneri, large intestine O157, ATCC, Shiga 301, Staphylococcus aureus cowan1, Aloe vera, LB medium, Oxford cup.

[0036] Experimental steps:

[0037]A Activate probiotics and pathogenic bacteria and culture them overnight.

[0038] B adjust the concentration of pathogenic bacteria to 10 8 About CFU / mL, take 100 μL of the bacterial solution and spread it on the LB plate.

[0039] C. After the bacteria solution is dried, carefully put a sterile Oxford cup on the plate; add probiotic aloe vera solution to the Oxford cup.

[0040] D Culture at 37°C for 6-8 hours, observe every 2-3 hours.

[0041] E Measure the diameter of the inhibition zone in the plate and take a picture.

[0042] The experimental results are shown in Table 1.

[0043] Table 1 Antibacterial results of probiotic aloe vera liquid

[0044]

[0045] The above results indicate...

Embodiment 3

[0046] Embodiment 3 (measurement and evaluation of antioxidant index)

[0047] 1. Related reagents: 0.2mmol / L DPPH ethanol; 150mmol / L pH 8.0 Tris-Hcl; 1.2mmol / L pyrogallol; 0.4% FeSO 4 :FeSO 4 ·7H 2 O; 1% ascorbic acid; 0.2mol / L sodium hydroxide; 10% trichloroacetic acid; 0.1% o-phenanthroline; pH 6.6 phosphate buffer; 0.1% ferric chloride.

[0048] 2. After determining the best condition of aloe fermented liquid, carry out fermentation under the best condition, and store it in a refrigerator at 4°C after fermentation.

[0049] 3. Determination of antioxidant index

[0050] A Determination of DPPH free radical scavenging ability

[0051] Take 2ml of probiotic aloe solution, add 2ml of 0.2mol / LDPPH ethanol solution, react at room temperature in the dark for 30min, then extract with an equal volume of chloroform, take the supernatant to measure OD at 517nm, and compare with deionized water plus DPPH solution.

[0052] Determination of B hydroxyl radical scavenging ability ...

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Abstract

The invention provides a probiotics aloe juice and a preparation method and applications thereof. A culture medium containing aloe vera leaf juice is adopted, lactobacillus plantarum, lactobacillus rhamnosus, lactobacillus delbrueckii, bifidobacterium infantis, bifidobacterium breve, and bifidobacterium adolescentis are inoculated to the culture medium to carry out fermentation; the fermentation broth has the functions of probiotics such as bacterium prevention, inflammation elimination, and the like, also has the functions of aloe extract such as bacterium prevention, oxidation prevention, and the like, and can be used to treat elytritis of women. By establishing dominant probiotics communities, the performance of vaginas on resisting external pathogenic bacteria is enhanced, moreover, the micro-ecological environment of vaginas is recovered, at the same time, drug resistance will not be generated, and the probiotics aloe juice is safe, does not have any side or toxic effect, and hasan important meaning for the treatment of gynecological diseases.

Description

technical field [0001] The invention relates to the technical field of gynecological medicine, in particular to a probiotic aloe liquid, its preparation method and application. Background technique [0002] Many microbial flora often co-exist in the cavity between the human body surface and the outside world, and they play an important physiological role beneficial to the host. The normal microbiota, host and environment are always in a state of dynamic balance, forming an interdependent and mutually restrictive system. Once this balance is disrupted, it may cause disease. [0003] Female lower reproductive tract diseases are mostly caused by the imbalance of vaginal micro-ecological environment, such as bacterial vaginosis, fungal vaginitis, trichomonas vaginitis and so on. The current measures for the treatment of vaginal infections are mainly to use antibiotics, metronidazole, estrogen and preparations to acidify the vagina to kill the pathogenic microorganisms in the va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896A61K36/886A61P15/02A61P31/04A61K35/747A61K35/745
CPCA61K35/745A61K35/747A61K36/886A61K36/896A61K2236/19A61K2236/31A61K2300/00Y02A50/30
Inventor 陈廷涛海志文
Owner NANCHANG UNIV
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