Method for preparing porous bacterial cellulose membrane

A technology of bacterial cellulose membrane and sodium carboxymethyl cellulose, which is applied in the field of preparation of porous bacterial cellulose membrane, can solve the problems of unfriendly environment, degraded cellulose chain, complicated operation process, etc., and the method is simple and easy, Improve air permeability and prevent sedimentation

Inactive Publication Date: 2018-11-02
NANJING UNIV OF SCI & TECH
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Problems solved by technology

[0003] In recent years, the modification and application of bacterial cellulose have emerged in an endless stream. Most of the methods have achieved certain results, but at the same time there are problems such as complicated operation process, unfriendly to the environment, and high cost.
Document 1 (Luo Chengcheng, Advances in Modification and Application of Cellulose. 2015: 767-773) summarizes three major methods of modifying cellulose, namely physical modification, chemical modification and biological modification. The above-mentioned methods are to obtain the finished cellulose product first, and then modify it later, which will easily cause the change of the cellulose crystal morphology, and make it lose the original high crystallinity, high strength and other excellent characteristics of cellulose.
Document 2 (Keshk S, etc.. Influence of lignosulfonate on crystal structure and productivity of bacterial cellulose in a static culture, 2006, 40 (1): 4-8) utilizes the method for adding lignosulfonate in the fermented liquid to bacteria Modification of cellulose, this method will produce agglomeration phenomenon while improving the binding force between cellulose fibers, so that the texture of the final modified product is not uniform enough
Document 3 (Zhao Yanfeng. Cellulose modification technology and progress. 2006,20(2):11-14) using cellulase to treat bacterial cellulose can improve the binding force between cellulose, but cellulase will degrade the fiber prime chain

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  • Method for preparing porous bacterial cellulose membrane
  • Method for preparing porous bacterial cellulose membrane
  • Method for preparing porous bacterial cellulose membrane

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preparation example Construction

[0017] The preparation and parameter monitoring of the seed liquid in the embodiment of the present invention can refer to the existing method, wherein the seed liquid can be prepared by the following steps:

[0018] The strains preserved at 4°C were kept at 30°C for 20 minutes, and a ring of strains was picked up with an inoculation loop and streaked on the solid plate medium, and the plate was cultured in a 30°C incubator for 36 hours. Composition of solid medium (g / 100mL): glucose 2.0, sucrose 1.0, magnesium sulfate 0.04, citric acid 0.11, sodium dihydrogen phosphate 0.25, peptone 1.0, agar 1.8, yeast extract powder 0.1. pH=6.0, sterilized at 121°C for 30 minutes;

[0019] The activated seeds were inoculated into a 500mL Erlenmeyer flask filled with 1 / 5 of the seed solution, and then placed in a shaker with reciprocating shaking at 120-160rpm for 48h. Seed solution composition (g / 100mL): glucose 2.0, ammonium sulfate 0.6, potassium dihydrogen phosphate 0.1, magnesium sulfa...

Embodiment 1

[0025] 1mg / 100mL of calcium carbonate and 40mg / 100mL of sodium carboxymethylcellulose were added to the fermentation broth, and the Acetobacter xylinum seed solution in the logarithmic growth phase was inoculated into the fermentation broth to carry out fermentation according to the inoculation amount of 10%. After the fermentation, the cellulose membrane was washed with 5 g / L acetic acid to remove calcium carbonate, followed by conventional rinsing and purification to obtain a porous bacterial cellulose membrane.

[0026] Adding calcium carbonate and sodium carboxymethyl cellulose to ferment the bacterial cellulose film obtained with no addition of calcium carbonate and sodium carboxymethyl cellulose to the scanning electron microscope of the bacterial cellulose film obtained by fermentation figure 1 shown, where figure 1 (a) and figure 1 (b) adding calcium carbonate and sodium carboxymethyl cellulose to ferment the bacterial cellulose film obtained, figure 1 (c) and figur...

Embodiment 2

[0029] Add 3mg / 100mL of calcium carbonate and 195mg / 100mL of sodium carboxymethylcellulose to the fermentation broth, and inoculate the Acetobacter xylinum seed solution in the logarithmic growth phase into the fermentation broth according to the inoculation amount of 10%. After the fermentation, the cellulose membrane was washed with 15 g / L acetic acid to remove calcium carbonate, followed by conventional rinsing and purification to obtain a porous bacterial cellulose membrane.

[0030] Add calcium carbonate and sodium carboxymethyl cellulose to ferment the bacterial cellulose film that obtains and do not add calcium carbonate and sodium carboxymethyl cellulose to ferment the XRD figure of the bacterial cellulose film that obtains figure 2 Shown, a is adding calcium carbonate and sodium carboxymethyl cellulose, b is not adding calcium carbonate and sodium carboxymethyl cellulose. It can be seen from the figure that the BC film has typical strong diffraction peaks at 2θ of 17...

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Abstract

The invention discloses a method for preparing a porous bacterial cellulose membrane. The method comprises the following steps: performing structural regulation by utilizing calcium carbonate as a pore-forming agent in the acetobacter xylinum fermentation process, and adding a thickening agent sodium carboxymethylcellulose to improve the viscosity of the fermentation system, so that the calcium carbonate is difficult to settle; removing calcium carbonate in the bacterial cellulose membrane by utilizing acetic acid after fermentation is ended, thereby obtaining the porous bacterial cellulose membrane which is completely free in calcium carbonate. The method disclosed by the invention is simple and feasible, the porosity of the bacterial cellulose membrane can be effectively regulated in situ by controlling the amount of the calcium carbonate and sodium carboxymethylcellulose, the air permeability of the prepared bacterial cellulose membrane is obviously improved while ensuring the original crystal form of cellulose, and the application field of the bacterial cellulose is further widened.

Description

technical field [0001] The invention relates to a preparation method of a porous bacterial cellulose membrane, which belongs to the technical field of biological materials. Background technique [0002] Bacterial Cellulose (BC for short) is an extracellular polysaccharide synthesized and secreted by a small number of microorganisms represented by Acetobacter xylinum. It has an ultrafine three-dimensional structure, and the width of the fiber is about 70-80nm. Structural modification of bacterial cellulose can further broaden the application field of bacterial cellulose. [0003] In recent years, the modification and application of bacterial cellulose have emerged in an endless stream, and most of the methods have achieved certain results, but at the same time there are problems such as complicated operation process, unfriendly to the environment, and high cost. Document 1 (Luo Chengcheng, Advances in Modification and Application of Cellulose. 2015: 767-773) summarizes three...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/02
CPCC12P19/04
Inventor 王海飞向忆南胡江婷雷开宇杨加志
Owner NANJING UNIV OF SCI & TECH
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