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Anti-bacterial and anti-inflammatory porous metal stent and preparation method and application thereof

A porous metal and metal technology, applied in application, coating, medical science, etc., can solve the problems of low drug loading, inability to improve the biological effect of tissue engineering substitutes, and induce inflammation, so as to ensure long-term drug efficacy and improve Drug loading performance, effect of enhancing binding force

Active Publication Date: 2018-11-06
WENZHOU MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method has a low drug loading and cannot meet the long-acting antibacterial requirements, and this type of material will still induce inflammation after implantation, affecting tissue repair
In addition, the loaded drug has a fixed release cycle. When the drug is released completely or fails to achieve the inhibitory effect of the drug, a series of complications will still occur. Therefore, the above method cannot improve the biological effect of tissue engineering substitutes.

Method used

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  • Anti-bacterial and anti-inflammatory porous metal stent and preparation method and application thereof
  • Anti-bacterial and anti-inflammatory porous metal stent and preparation method and application thereof
  • Anti-bacterial and anti-inflammatory porous metal stent and preparation method and application thereof

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preparation example Construction

[0040] Such as figure 1 Shown, the preparation method of the antibacterial and anti-inflammatory porous metal stent of an embodiment of the present invention, comprises the following steps:

[0041] S101: Surface treatment is performed on the porous metal support.

[0042] Chemical bonds are introduced by surface treatment of porous metal scaffolds. The surface treatment method can be methods such as spray coating, spin coating, printing, dipping, sol-gel technology and the like.

[0043] In one embodiment, a silane coupling agent is used to aminate the surface of the metal stent with the nano-array structure.

[0044] Amination treatment of the surface of the porous metal support by using a silane coupling agent can successfully introduce covalent bonds on the porous metal support, which can increase the chemical reaction sites on the one hand, and increase the interaction between functional molecules and the porous metal support on the other hand. The binding force and in...

Embodiment 1

[0074] (1) Porous metal stents are prepared by anodic oxidation, wherein the electrolyte is: 10% glycerin (containing 0.5% w / v ammonium fluoride) aqueous solution, the voltage is 40-60V, and the electrolysis time is 8-12h;

[0075] (2) Soak the above-mentioned porous metal stent in 10 mM APTES solution prepared by hexane solution to complete the amination treatment on the surface of the porous metal stent;

[0076] (3) Make heparin sodium (molecular weight 200KDa) into 1 mg / mL aqueous solution, add corresponding amounts of EDC (molar ratio 1.5:1) and NHS (molar ratio 1.2:1), stir, and react at room temperature. After the reaction is complete, Separation to obtain a porous metal stent containing a heparin layer;

[0077] (4) 0.7M CeCl 3 ·7H 2 Add O to 5ml of 2% (w / v) low molecular weight chitosan, stir for 20min, then add 0.1mL of ammonia water (28-30%), centrifuge at 5000rpm for 10min at room temperature, collect the supernatant, and use a sliding dialysis box Carry out dia...

Embodiment 2

[0080] (1) Porous metal stents are prepared by anodic oxidation, wherein the electrolyte is: 10% glycerin (containing 0.5% w / v ammonium fluoride) aqueous solution, the voltage is 40-60V, and the electrolysis time is 8-12h;

[0081] (2) Soak the above-mentioned porous metal stent in 15 mM APTES solution prepared by hexane solution to complete the amination treatment on the surface of the porous metal stent;

[0082] (3) Make heparin sodium (molecular weight 200KDa) into 1 mg / mL aqueous solution, add corresponding amounts of EDC (molar ratio 1.5:1) and NHS (molar ratio 1.2:1), stir, and react at room temperature. After the reaction is complete, Separation to obtain a porous metal stent containing a heparin layer;

[0083] (4) 5mL polyallylamine hydrochloride (37.4mg / mL), add 15mL 10mM KMnO 4 solution, stirred for 10min, centrifuged to collect the precipitate, washed 5 times with pure water to obtain the manganese dioxide product, and 5ml of 2% (w / v) low molecular weight chitosa...

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Abstract

The invention provides an anti-bacterial and anti-inflammatory porous metal stent and a preparation method and application thereof. The preparation method includes the steps that a porous metal stentis subjected to surface treatment; the porous metal stent obtained after surface treatment is reacted with heparin, and a heparin layer is formed on the surface of the porous metal stent; the heparinlayer is modified with a polysaccharide complex of metal oxide nanoparticles and an antibacterial agent, and then the anti-bacterial and anti-inflammatory porous metal stent is obtained. By means of the method, the porous metal stent with the biological effect of long-acting antibacterial oxidative stress resistance can be obtained, and postoperative tissue repair is effectively improved.

Description

technical field [0001] The invention relates to the technical field of biomaterials, in particular to an antibacterial and anti-inflammatory porous metal support and a preparation method and application thereof. Background technique [0002] At present, the implantation of metal stents is an indispensable tool in surgical treatment, but the use of this artificial hard tissue substitute is prone to a series of complications, which will affect the repair of postoperative tissues. In order to avoid such symptoms, usually Intraoperative or postoperative use of relevant anti-inflammatory and antibacterial drugs for control, but this method is less effective and will bring a certain burden on the patient's body. In order to improve the success rate of tissue repair and surgery, researchers have been working on developing new functional materials or functional composite materials. At present, the more common method is to load antimicrobial drugs on the surface of metal stents by p...

Claims

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Application Information

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IPC IPC(8): A61L27/04A61L27/06A61L27/34A61L27/54A61L27/56A61L24/00A61L24/02A61L24/08A61L15/18A61L15/28A61L15/42A61L15/44A61L15/46
CPCA61L15/18A61L15/28A61L15/425A61L15/44A61L15/46A61L24/0015A61L24/0031A61L24/0036A61L24/02A61L24/08A61L27/045A61L27/047A61L27/06A61L27/34A61L27/54A61L27/56A61L2300/102A61L2300/232A61L2300/406A61L2300/41A61L2300/45A61L2300/602A61L2300/61A61L2430/02A61L2430/12C08L5/10C08L5/08
Inventor 师帅刘劲松郑倩倩陈浩
Owner WENZHOU MEDICAL UNIV
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