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Probiotics microcapsule as well as preparation method and application thereof

A technology of probiotics and microcapsules, applied in application, animal feed, additional food elements, etc., can solve the problems of loss of coating protection, difficulty in forming sphericity, and damage to probiotic cells, so as to promote colonization in the intestinal tract, Avoid decomposition, high mechanical strength effect

Inactive Publication Date: 2019-01-11
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

CN1969889A discloses a method for preparing enteric-coated multilayer-coated probiotic microcapsules by extrusion method. Most documents compare the advantages and disadvantages of emulsification method and extrusion method, and think that extrusion method is difficult to form sphericity when preparing microcapsules. And it is not conducive to the outer coating; generally speaking, the particle size of microcapsules prepared by extrusion method is relatively large (2-3mm), and the too large particles are only suitable for monogastric animals. For ruminants, it is easy to grind through the omasum. Break the microcapsules and lose the protective effect of the envelope
CN105105144A discloses a method for preparing microcapsules by emulsification method, but the embedding rate of emulsification method itself is low, and excessive shear force in the emulsification process will also cause damage to probiotic cells
CN103283975A discloses a method for preparing probiotic microcapsules by spray drying. This method has little effect on high-temperature-resistant microorganisms, but for temperature-sensitive lactic acid bacteria, if the temperature of the spray dryer exceeds 40°C, the live bacteria of lactic acid bacteria In actual operation, the rate of viable bacteria will drop by an order of magnitude for every 10°C increase in temperature
CN103275966A discloses a pre-fermentation coating, and the microencapsulation technology for controlling the proliferation and metabolism of bacteria through re-cultivation after coating has been well developed, but the preparation cost is high

Method used

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  • Probiotics microcapsule as well as preparation method and application thereof
  • Probiotics microcapsule as well as preparation method and application thereof
  • Probiotics microcapsule as well as preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The preparation of embodiment 1 plantarum lactobacillus (single bacterium) microcapsule

[0046] 1) Activation and proliferation of bacteria:

[0047] Inoculate Lactobacillus plantarum JCM-1149 (derived from the Japanese strain preserved in the Microbiology Laboratory of the Agricultural College of China Agricultural University) into MRS liquid medium for three generations of activation culture, and use a tube centrifuge (above 10000r / min) to centrifuge for 10-15min. Collect the slime.

[0048] 2) Prepare the bacteria slime mixture:

[0049] Take 15ml of bacteria slime and add 45ml of 20% skimmed milk powder aqueous solution (bacteria slime: 20% skimmed milk powder solution = 1:3), mix well, and obtain the bacteria slime mixture with protective agent added.

[0050] 3) Granulation:

[0051] Fully mix microcrystalline cellulose blank core and sodium alginate as a matrix (60g microcrystalline cellulose and 5.4g sodium alginate are fully mixed), add 60ml of fungus slime...

Embodiment 2

[0068] The preparation of embodiment 2 composite lactic acid bacteria (composite bacteria) microcapsules

[0069] 1) Activation and proliferation of bacteria:

[0070] The compound lactic acid bacteria are mainly composed of Lactobacillus, Lactococcus and Bacillus (provided by the Microbiology Laboratory of the Agricultural College of China Agricultural University), inoculated in the MRS liquid medium for activation and cultivation for three generations, using a tubular centrifuge (above 10000r / min ) by centrifugation for 10-15min to collect the sludge.

[0071] 2) Prepare the bacteria slime mixture:

[0072] Take 1 kg of the above-mentioned processed fungus slime and add 3 kg of 20% skim milk powder aqueous solution (sludge: 20% skim milk powder solution = 1:3), and mix well to obtain the fungus slime mixture with protective agent added.

[0073] 3) Granulation:

[0074] The microcrystalline cellulose blank ball core and sodium alginate were fully mixed as a matrix for gra...

Embodiment 3

[0093] The performance test of embodiment 3 microcapsule products

[0094] The existing reports all use in vitro simulation of the gastrointestinal tract environment to test whether the coated bacteria can tolerate the environment of gastric acid and bile salts, and finally target release. However, this study adopts the method of in vivo verification to truly evaluate the biological process experienced by lactic acid bacteria when they play a probiotic role. The specific implementation is as follows:

[0095] For the test, 4 three-parity Holstein dry cows with good body condition and similar body weight were selected in the Zhongdi Breeding Stock Cow Science and Technology Park in Shunyi District, Beijing. Permanent rumen fistula and duodenal fistula were installed for rumen and duodenum Collection of contents. After one month of postoperative care, the animal body returned to a normal state for experimentation.

[0096] The experiment was divided into three phases, each for...

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Abstract

The invention provides a probiotics microcapsule as well as a preparation method and application thereof. According to the preparation method, the microencapsulation preparation of probiotics cells iscarried out by virtue of a natural controlled release material with good biocompatibility, a high molecular material with pH value sensitivity and a mild process based on a microcapsule preparation technique of ruminants. By granulating microcrystalline cellulose blank pellet core as a pellet-granulated film-forming core granule, the problems of low sphericity degree and poor particle uniformityin the granulation process are effectively solved, and the effects of a dilution-adhesion-disintegration three-purpose agent are simultaneously considered. By utilizing a double-layer coating technique, the probiotics microcapsule can respectively resist the decomposition of rumen microorganisms and the erosion of true gastric acid, so that bacteria can reach the intestinal tract as much as possible, and the effective field planting of microorganisms in the intestinal tract of a milk cow. The method has the beneficial effects that the operation is simple, the industrial microencapsulation preparation can be realized through the double-layer coating of a fluidized bed, and a set of effective process parameters are established for the industrial production of coated probiotics cells.

Description

technical field [0001] The invention relates to the field of feed additives, in particular to probiotic microcapsules and a preparation method and application thereof. Background technique [0002] Antibiotics have been used in feed for nearly 60 years. They are popular due to their remarkable effect and low price, but they also bring hidden dangers, such as drug resistance, residues in livestock products and food safety risks, animal immunosuppression and secondary For severe infections, the use of antibiotics has become a global problem. The European Union has banned the use of antibiotics in feed since 2006. After the ban on antibiotics in feed, the main substitute for antibiotics was the application of probiotics. As we all know, a class of micro-ecological living bacteria preparations widely used at present is mainly developed with various probiotics such as lactic acid bacteria, bifidobacteria, enterobacteria and bacillus, and its main function is to supplement the am...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/10A23K40/35A23K10/18A23K20/163
CPCA23K10/18A23K20/163A23K40/35A23K50/10
Inventor 李胜利郭春燕王雅晶纪守坤严慧刘晶晶都文蒋涛程发祥
Owner CHINA AGRI UNIV
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