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Method for improving anaerobic fermentation efficiency of mushroom residue by white rot fungus pretreatment technology

A white-rot fungus and anaerobic fermentation technology, applied in the field of organic solid waste recycling, can solve the problems of lower anaerobic digestion efficiency and hinder the use of substrates by microorganisms, and achieve good application prospects, high product added value, and raw material sources wide range of effects

Inactive Publication Date: 2019-01-25
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The amorphous binding of lignin in the mushroom residue structure to cellulose limits the accessibility of cellulose and enzymes during the biodegradation process, hindering the further use of substrates by microorganisms, resulting in a decrease in the efficiency of anaerobic digestion.

Method used

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  • Method for improving anaerobic fermentation efficiency of mushroom residue by white rot fungus pretreatment technology

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Experimental program
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Effect test

Embodiment 1

[0021] (1) Take a certain amount of mushroom slag from a certain mushroom factory, whose cellulose content is 12.3%, hemicellulose content is 8.3%, and lignin content is 21.7%, and it is dried in an oven at 60°C for 48 hours, then mechanically pulverized, sieved, and granulated. The diameter is 2.5-1.0cm, and the sieved mushroom dregs are sterilized at 121°C for 30 minutes for subsequent pretreatment;

[0022] (2) Under aseptic and room temperature conditions, Pleurotus Sajor Caju (MES 03464) was inoculated into a reactor containing sterilized mushroom dregs at a mass ratio of 0.1, and the inoculation volume accounted for about 1 / 3 of the entire reactor. Cultivate for 6 weeks under aerobic conditions at 25°C and 75% humidity.

[0023] (3) get the mushroom dregs that obtain in step (2) and put into fermenter, add distilled water and be configured into the mixed solution that solid content rate is 15%; Add inoculated sludge in mixed solution, mixed solution volume and inoculated...

Embodiment 2

[0026] (1) Take a certain amount of mushroom dregs from a certain mushroom factory, whose cellulose content is 17.5%, hemicellulose content is 6.8%, and lignin content is 24.7%, and it is dried in an oven at 60°C for 48 hours, then mechanically crushed, sieved, and granulated. The diameter is 3.0-1.8cm, and the sieved mushroom dregs are sterilized at 121°C for 30 minutes for subsequent pretreatment;

[0027] (2) Under aseptic and room temperature conditions, Pleurotus Sajor Caju (MES 03464) was inoculated into a reactor containing sterilized mushroom dregs at a mass ratio of 0.1, and the inoculation volume accounted for about 1 / 3 of the entire reactor. Cultivate for 6 weeks under aerobic conditions at 25°C and 75% humidity.

[0028] (3) get the mushroom dregs that obtain in step (2) and put into fermenter, add distilled water and be configured into the mixed solution that solid content rate is 12%; Add inoculated sludge in mixed solution, mixed solution volume and inoculated s...

Embodiment 3

[0031] (1) Take a certain amount of mushroom dregs from a certain mushroom factory, whose cellulose content is 15.2%, hemicellulose content is 8.1%, and lignin content is 19.3%, and it is dried in an oven at 60°C for 48 hours, then mechanically crushed, sieved, and granulated. The diameter is 2.7-1.6cm, and the sifted mushroom dregs are sterilized at 121°C for 30 minutes for subsequent pretreatment;

[0032] (2) Under aseptic and room temperature conditions, Pleurotus Sajor Caju (MES 03464) was inoculated into a reactor containing sterilized mushroom dregs at a mass ratio of 0.1, and the inoculation volume accounted for about 1 / 3 of the entire reactor. Cultivate for 6 weeks under aerobic conditions at 25°C and 75% humidity.

[0033] (3) get the mushroom dregs that obtain in step (2) and put into fermenter, add distilled water and be configured into the mixed solution that solid content rate is 15%; Add inoculated sludge in mixed solution, mixed solution volume and inoculated s...

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Abstract

The invention relates to the technical field of organic solid waste recycling, in particular to a method for improving the hydrolysis acidification efficiency of mushroom residue by a white-rot funguspretreatment technology. A mushroom residue with high lignin content is use as a pretreatment substrate, that dried mushroom residue is mechanically pulverized, and the mushroom residue is sterilizedunder the condition of 0.1-0.5 mass inoculation of Pleurotus Sajor Caju (MES 03464) and pretreatment with 4-6 weeks. Anaerobic digestion sludge is inoculated sludge, and the solid content is 12%-18%,mixing the mixture with the inoculated sludge according to the mass ratio of volatile organic compounds (VOCs) 1:2, and filling the mixture with nitrogen 3-5min; the reaction temperature was 37 DEG Cand the stirring speed was 150-160r / min, fermentation in anaerobic environment for 6-9d; the centrifuge was used to centrifuge the fermented mixture. The centrifuge speed was 15000r / min, and the supernatant after centrifugation was VFA fermentation broth. The process is simple in operation and low in economic cost, and has a good application prospect. The invention provides a new method for mushroom residue recycling disposal.

Description

technical field [0001] The invention relates to the technical field of organic solid waste recycling, in particular to a method for selectively decomposing lignin by using white-rot fungus pretreatment technology to promote anaerobic fermentation of mushroom dregs to produce volatile fatty acids (VFA). Background technique [0002] Mushroom slag is a by-product of the mushroom production process. It is mainly composed of cellulose, hemicellulose and lignin. It has a high carbon-to-nitrogen ratio and is rich in nitrogen, phosphorus, potassium and other elements. At present, most mushroom dregs lack effective treatment measures and are randomly piled up, resulting in waste of resources and easy to cause secondary pollution to the environment. Although a small amount of mushroom dregs are used as soil amendments or animal feed, these methods have low disposal capacity and low added value of products, which cannot effectively alleviate the problem of a large amount of mushroom d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/54C12P7/52C12P7/40C12R1/645
CPCC12P7/40C12P7/52C12P7/54
Inventor 房玮张涛曲波刘娅王中玮姜宁
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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