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A kind of microbial bacterial agent for aquaculture sewage treatment

A technology for microbial inoculants and aquaculture sewage, which is applied in biological water/sewage treatment, microorganisms, water/sludge/sewage treatment, etc. It can solve the problems of inability to discharge directly, high operating costs, and large treatment costs, so as to reduce environmental damage, lower operating costs, and excellent practical performance

Active Publication Date: 2021-11-26
上海国龙生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high concentration of livestock and poultry breeding wastewater, which contains a large number of pathogenic bacteria, parasite eggs, antibiotics and other hormones and heavy metal inhibitors, it cannot be directly discharged or used for farmland irrigation. If it is directly discharged, it will cause serious pollution to the environment. cause a series of environmental problems
The cost of conventional industrial water treatment is too high, and the current wet fermentation biogas engineering technology has problems such as large discharge of biogas slurry and cannot be digested. Therefore, the treatment of antibiotic-containing breeding wastewater is still the current pollution of livestock and poultry breeding. The thorny problem of prevention and control; the activated sludge method, the sludge production is large, and the sludge is difficult to control; the MBR biofilm method, the membrane is easy to clog, and the operating cost is high. There are deficiencies and cannot meet the needs of rapid social development.

Method used

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  • A kind of microbial bacterial agent for aquaculture sewage treatment
  • A kind of microbial bacterial agent for aquaculture sewage treatment

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Experimental program
Comparison scheme
Effect test

preparation example Construction

[0054] The preparation method of bacillus subtilis is:

[0055] The medium is:

[0056] Sucrose 10kg / T, glucose 0.05kg / T, soybean powder 8kg / T, peptone 5kg / T, beef extract 1 kg / T, ammonium sulfate 1kg / T, sodium chloride 5kg / T;

[0057] The medium fermentation conditions are: 37°C, pH 7.0, tank pressure 1Kg, ventilation rate 80 cubic meters per hour, stirring 80rpm, dissolved oxygen 30%, fermentation time about 24 hours;

[0058] The preparation method of bacillus cereus is:

[0059] The medium is:

[0060] Sucrose 10kg / T, soybean powder 8kg / T, peptone 5kg / T, beef extract 1kg / T, sodium chloride 3kg / T, ammonium sulfate 1kg / T, potassium dihydrogen phosphate 2kg / T;

[0061] The medium fermentation conditions are: 37°C, pH 7.0, tank pressure 1Kg, ventilation rate 80 cubic meters per hour, stirring 80rpm, dissolved oxygen 30%, fermentation time about 24 hours;

[0062] The preparation method of bacillus amyloliquefaciens is:

[0063] The medium is:

[0064] Sucrose 10kg / T, soy...

Embodiment 1

[0087] A highly efficient sewage treatment bacterial agent, composed of Bacillus subtilis 5.0 × 10 9 cfu / ml, Bacillus cereus is 1.0×10 9 cfu / ml, Bacillus amyloliquefaciens 1.5×10 9 cfu / ml, Candida krusei is 5.0×10 9 cfu / ml, Candida wrinkled 5.0×10 9 cfu / ml, Bacillus methanolica 1.2 × 10 9 cfu / ml, Rhodopseudomonas palustris 1.5×10 9 cfu / ml, Pseudomonas syringae 1.5×10 9 cfu / ml, Pseudomonas oligooxygen 1.0×10 9 cfu / ml Bacillus subtilis is 5.0×10 9 cfu / ml, Bacillus cereus is 1.0×10 9 cfu / ml, Bacillus amyloliquefaciens 1.5×10 9 cfu / ml, 5.0×10 for Candida krusei 9 cfu / ml, Candida wrinkled 5.0×10 9 cfu / ml, Bacillus methanolica 1.2×10 9 cfu / ml, Rhodopseudomonas palustris 1.5×10 9 cfu / ml, Pseudomonas syringae 1.5×10 9 cfu / ml, Pseudomonas oligooxygen 1.0×10 9 cfu / ml and water composition. After treatment, COD≤150mg / ml, ammonia nitrogen≤70mg / ml, total phosphorus≤7mg / ml.

[0088] The addition amount is 2%, and the micro-aquatic bacterium is supplemented once every 6 month...

Embodiment 2

[0092] COD degradation experiment

[0093] The fermentation media of Bacillus methanolica and Rhodopseudomonas paluscans were shaken, and then the number of colonies was measured to ensure that the number of colonies was consistent. Composite strains are prepared in a ratio of 1:1 so that the amount of the single strain is the same as that of the single strain. Adjust the sewage composition, prepare COD2000mg / ml and ammonia nitrogen1000mg / ml in proportion with compounds, and aerate regularly according to the treatment process. Regularly sample and measure COD. Analysis and comparison of sewage COD degradation rate of different batches of bacterial strains were tested, and the strain with the highest degradation rate was screened. The results are shown in Table 1.

[0094] Table 1

[0095]

[0096] Both Bacillus methanolica and Rhodopseudomonas palustris have COD degradation effects, and the mixed use of the two bacteria has a better effect. The degradation rate reaches ...

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Abstract

The invention discloses a microbial bacterial agent for aquaculture sewage treatment, which comprises ammonia nitrogen degrading bacteria, COD degrading bacteria, phosphorus removing bacteria, ammonia nitrogen degrading bacteria, COD degrading bacteria, phosphorus removing bacteria and appropriate amount of water are mixed to prepare a finished sewage treatment bacterial agent; Treating aquaculture sewage, reducing operating costs, making treated water discharge up to standards, and reducing damage to the environment, compounding multiple functional microbial agents to improve aquaculture water quality is of positive significance to the field of agricultural aquaculture technology. It has excellent practical performance and is a kind of It is a very good innovative solution and has great market promotion prospects.

Description

technical field [0001] The invention relates to sewage treatment technology, in particular to a microbial bacterial agent for aquaculture sewage treatment. Background technique [0002] With the rapid development of the aquaculture industry, especially intensive aquaculture has caused serious water pollution, there have been more and more reports of water quality deterioration caused by aquaculture in recent years. The main pollutants in the aquaculture waters are feed residues, excreta, and the use of some drugs. The accumulation of nitrogen and phosphorus nutrients in the feed will lead to eutrophication of the water body, and it will also lead to chemical oxygen demand, ammonia nitrogen, and nitrite in the water body. Nitrate etc. seriously exceed the standard, direct discharge will affect the surrounding environment. By 2015, the total number of livestock and poultry breeding in the country will be about 1.4 billion (pig equivalent); according to the current level of po...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F103/20C12R1/125C12R1/085C12R1/07C12R1/38
CPCC02F3/34C02F2103/20C12N1/20
Inventor 钟诚雷雨姜蒙曾伟吴庆儒
Owner 上海国龙生物科技有限公司