Silk fibroin/manganese dioxide composite micro-sphere drug carrier with core-shell structure and preparation method

A technology of silk fibroin and manganese dioxide, which is applied in the direction of non-active ingredients of polymer compounds, drug combinations, and pharmaceutical formulations, can solve problems such as unstable properties of nanoparticles, harsh reaction conditions, and complicated nanoparticle synthesis technology, and achieve The effect of excellent singlet oxygen generation ability, simple process, and controllable drug release ability

Active Publication Date: 2019-09-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to overcome the existing MnO 2 Nanoparticle synthesis technology is complex, reaction conditions are harsh, and pure MnO 2 Due to defects such as unstable properties of nanoparticles, the present invention provides a method for preparing silk protein / manganese dioxide composite microspheres with a core-shell structure by self-assembly using silk protein microspheres as a template

Method used

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  • Silk fibroin/manganese dioxide composite micro-sphere drug carrier with core-shell structure and preparation method
  • Silk fibroin/manganese dioxide composite micro-sphere drug carrier with core-shell structure and preparation method
  • Silk fibroin/manganese dioxide composite micro-sphere drug carrier with core-shell structure and preparation method

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Embodiment 1

[0040] The preparation method of the silk fibroin / manganese dioxide composite microsphere drug carrier of the core-shell structure in this embodiment sequentially includes the following steps:

[0041] (1) Take an appropriate amount of silkworm cocoon shells, wash and air dry, and use the existing method for degumming. Subsequently, the degummed silk fibers are sequentially subjected to the existing steps of dissolution, dialysis, and concentration to obtain the silk fibroin aqueous solution;

[0042] (2) Adjust the silk fibroin concentration in step (1) to 2 mg / mL (the solvent is water, the same as in other embodiments), and place 10 mL in the reaction vessel. Slowly add 1 mL of isopropanol dropwise to the silk protein solution, and continue to stir for 30 min to make it uniform.

[0043] (3) Place the mixed solution of step (2) in a -80°C refrigerator, freeze for 12 hours, and then allow it to thaw naturally at room temperature to obtain a milky white silk protein suspension. The...

Embodiment 2

[0049] The preparation method of the silk fibroin / manganese dioxide composite microsphere drug carrier of the core-shell structure in this embodiment sequentially includes the following steps:

[0050] (1) Take an appropriate amount of silkworm cocoon shells, wash and dry, and then degummed them. Subsequently, the degummed silk fibers are successively subjected to the steps of dissolution, dialysis, and concentration to obtain the silk fibroin aqueous solution;

[0051] (2) Adjust the silk fibroin concentration in step (1) to 2%, take 5 mL and place in the reaction vessel, add 2 mL of absolute ethanol and stir evenly.

[0052] (3) Place the mixed solution of step (2) in a refrigerator at -20°C, freeze for 24 hours, and naturally thaw at room temperature to obtain a silk protein suspension. The silk protein suspension was centrifuged, washed and ultrasonically processed to obtain silk protein microspheres with a diameter in the range of 200-500 nm, and resuspended with 10 mL of deion...

Embodiment 3

[0055] Example 3 (In situ drug loading)

[0056] The preparation method of the silk fibroin / manganese dioxide composite microsphere drug carrier of the core-shell structure in this embodiment sequentially includes the following steps:

[0057] (1) Take an appropriate amount of silkworm cocoon shells, wash and dry, and then degummed them. Subsequently, the degummed silk fibers are successively subjected to the steps of dissolution, dialysis, and concentration to obtain the silk fibroin aqueous solution;

[0058] (2) Adjust the silk fibroin concentration in step (1) to 2mg / mL, take 10mL and place it in the reaction vessel, add 2mg of doxorubicin hydrochloride (DOX) to the silk fibroin solution, and ultrasonically disperse it with the silk The protein protein is mixed uniformly, and then 1 mL of isopropanol is slowly added dropwise to the reaction solution, and stirring is continued for 30 minutes to make it uniform.

[0059] (3) Place the mixed solution of step (2) in a -80°C refrigera...

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Abstract

The invention discloses a preparation method of a silk fibroin/manganese dioxide composite micro-sphere drug carrier with a core-shell structure. The preparation method includes the steps: (1) adding aqueous-organic solvents into silk fibroin water solution, removing the solvents after freezing-natural thawing to obtain a silk fibroin nanosphere; (2) uniformly mixing the obtained silk fibroin nanosphere, potassium permanganate and polyallylamine hydrochloride, finishing incubation and removing the solvents to obtain the silk fibroin/manganese dioxide composite micro-sphere drug carrier with the core-shell structure taking silk fibroin as an inner layer and manganese dioxide as an outer layer. The preparation method is simple in process and simple and convenient in operation, the surface of a prepared silk fibroin/manganese dioxide composite micro-sphere is rough, excellent performances of the silk fibroin and the manganese dioxide are combined, drug loading rate is high, the preparation method can respond to controllable release of tumor microenvironments, hydrogen peroxide in a tumor environment is degraded to generate oxygen, chemical and photodynamic therapy efficiency of tumors is improved, and the preparation method has a wide application prospect in the field of tumor therapy and drugs.

Description

Technical field [0001] The invention belongs to the field of biomedical materials, and specifically relates to a preparation method of a silk fibroin / manganese dioxide composite microsphere drug carrier with a core-shell structure. Background technique [0002] Sol-like manganese dioxide (MnO 2 ) Nanoparticles have become emerging materials in the field of biomedicine due to their specific catalytic properties and good biocompatibility. In the field of tumor treatment, manganese dioxide nanoparticles can specifically react with hydrogen peroxide in the tumor microenvironment to catalyze its degradation to produce oxygen and water, thereby improving the hypoxic environment and lowering the hypoxia-inducible factors, which both weaken the tumor Tissue drug resistance also helps photosensitizers convert oxygen into singlet oxygen, and at the same time improves the efficiency of tumor chemotherapy and photodynamic therapy. H rich in tumor microenvironment + And glutathione (GSH) can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/42A61K47/02A61K47/64A61K47/52A61K41/00A61K31/704A61K31/337A61P35/00
CPCA61K9/501A61K9/5052A61K31/337A61K31/704A61K41/0071A61K47/52A61K47/64A61P35/00A61K2300/00
Inventor 杨明英王捷陈玉银陈玉平范鑫
Owner ZHEJIANG UNIV
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