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Bacillus cereus to prevent and treat Poplar canker disease and biological control preparation and method to prevent and treat Poplar canker disease

The technology of Bacillus cereus and biological control preparation is applied in the field of Bacillus cereus and biological control preparations for preventing and treating poplar canker, which can solve the problems of polluting the environment, time-consuming, and high prevention and control costs, and achieves fast growth speed and promotes The effect of poplar growth and efficient control

Pending Publication Date: 2019-09-20
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are problems such as high cost of prevention and control, long time consumption, and environmental pollution

Method used

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  • Bacillus cereus to prevent and treat Poplar canker disease and biological control preparation and method to prevent and treat Poplar canker disease
  • Bacillus cereus to prevent and treat Poplar canker disease and biological control preparation and method to prevent and treat Poplar canker disease

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preparation example Construction

[0033] And, also provide the preparation method of the biological control agent that is used for preventing and treating poplar canker, comprise the following steps:

[0034] Step 1): preparing the seed liquid of Bacillus cereus;

[0035] Step 2): Inoculate the seed solution prepared in S1 into a solid medium, and culture at a constant temperature of 28-30°C;

[0036] Step 3): Mix the culture cultured in S2 with sterile water at a mass ratio of 1:15, filter, inoculate the filtrate into a large amount of fermentation medium, and ferment at room temperature 28-30°C and relative humidity above 85%. fermentation in the chamber.

[0037] In a specific embodiment of the present invention, the above-mentioned preparation method comprises the following steps:

[0038] Step 1: Transplant the spores of Bacillus cereus into LB liquid medium, shake and culture on a shaker at 28-30°C for 3-5 days to obtain seed liquid;

[0039] The second step: inoculate the seed liquid prepared in the ...

Embodiment 1

[0047] 1. Bacillus cereus ( B. cereus ) Isolation and purification of SM02:

[0048] Bacillus cereus of the present invention ( B. cereus ) SM02 is obtained from saline-alkali soil by dilution plate method and plate streak method. The separation method is as follows:

[0049] Step 1: Isolation of Bacillus cereus: soil sample collection, saline-alkali soil was collected in Dongying, and mixed; weighed 1 g of soil in 100 mL of sterile water, placed in a shaker at 30°C at 150 rpm for 10 min, Then place it in a 60°C water bath and incubate for 30min, take 100μL of 10 -2 、10 -3 、10 -4 The diluted solution is spread on the LB medium plate, and each gradient is coated in three parallels. After culturing at 30°C for 2 days, the microbial colonies of different forms on the LB medium are picked and streaked on the LB medium plate, and observed regularly The growth of the colony; then, the Bacillus cereus strain was purified by the plate streak method, and the number was preserved...

Embodiment 2

[0062] 1. Fermentation process of Bacillus cereus:

[0063] The formula of LB liquid medium is 10 g of tryptone, 5 g of yeast extract, 10 g of sodium chloride, 1000 mL of distilled water, and the pH is adjusted to 7.0-7.2;

[0064] The solid material is rice husk: corn flour: soybean meal flour: bran=55:25:15:5; the inorganic salt solution is 20% potassium dihydrogen phosphate, 5% magnesium sulfate, 10% ammonium sulfate, 65% light calcium carbonate ; The solid-liquid ratio is 98.7:0.3 (mass ratio).

[0065] Bacillus cereus ( B. cereus ) Mass solid fermentation process of strain SM02:

[0066] The first step: culture of bacterial seed liquid: the Bacillus cereus strain ( B. cereus ) SM02 Pick a small amount of bacteria from the slant of the test tube, transfer to LB liquid medium, shake and culture at 30°C for 3-5 days, this is the seed solution;

[0067] Step 2: Cultivation of solid production strains: Inoculate the seed liquid into solid medium (500 mL Erlenmeyer flask)...

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Abstract

The invention discloses Bacillus cereus to prevent and treat Poplar canker disease and a biological control preparation and method to prevent and treat Poplar canker disease. The Bacillus cereus herein is a Bacillus cereus strain SM02 isolated from saline-alkaline soil and applicable to biological control preparations. The Bacillus cereus strain features high growth speed, high spore production, good stress resistance, availability for high-speed mass colonization on plant surface and the like; therefore, the Bacillus cereus strain has a good application prospect. In addition, the biological control preparation prepared with the Bacillus cereus strain is suitable for preventing and treating Poplar canker disease efficiently, is also suitable for promoting Poplar growth efficiently, and is a biological control preparation having an excellent application prospect. The biological control preparation, as a microbial preparation, may act as a biological pesticide or biological fertilizer to prevent and treat Poplar canker disease.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to Bacillus cereus for preventing and treating poplar canker, as well as a biological control preparation and method. Background technique [0002] Poplar is an important industrial plantation tree species in my country. Due to long-term continuous cropping management, the productivity of forest land has declined generation by generation, and frequent diseases have seriously affected the stability and sustainable development of poplar production. Poplar canker, also known as vesicular canker, is one of the most serious diseases on poplar, and its pathogenic fungi include Botrytis ( Botryosphaeria ), Ulcerospora ( Dothichiza ), Scutellaria ( Coniothyrium ), Solanum ( Valsa ), Cyclospora ( Coryneum ), from the genus Erythroxylum ( Nectria ), Phoma ( Phoma ) etc.; in my country, it is mostly reported as Botrytis ( Botryosphaeria ), the genus of...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/085
CPCA01N63/00C12N1/20C12N1/205C12R2001/085
Inventor 马丙尧马江燕马春艳高伟朱升祥牛赡光马海林杜振宇刘方春李宗泰李宜明刘幸红张淑静王霞张文馨高嘉
Owner SHANDONG FOREST SCI RES INST
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