Preparation method of lentinan component with immunocompetence as well as product and application thereof

Abstract

The invention discloses a preparation method of a lentinan component with immunocompetence, which comprises the following steps: (1) coarsely crushing dried lentinan, and carrying out superfine crushing; (2) extracting the product in the step (1) with hot water, and collecting lentinan aqueous extract; (3) treating the lentinan aqueous extract with a micro-filtration membrane assembly with the pore diameter of 50-100 nm for solid-liquid separation, or filtering the lentinan aqueous extract with a PPS filter bag to remove insoluble substances and a lentinan extract B in the lentinan aqueous extract; and (4) performing ultrafiltration treatment on the lentinan extracting solution B in the step (3) with three ultrafiltration membrane assemblies. According to the invention, a multi-stage crushing and multi-stage filtering mode is adopted; effective separation of lentinan components is realized; meanwhile, convenience is provided for recovering trehalose and mannitol which are rich in smallmolecular components; the technical scheme is simple and easy to operate, large-scale preparation and production are easy to realize, the production process is green and safe, and the lentinan component obtained by the technical scheme contains less than or equal to 1% of protein, less than or equal to 3.2% of flavone and less than or equal to 2.8% of reducing sugar.

Description

technical field [0001] The invention relates to the field of extraction and purification of natural products, in particular to a preparation method of a lentinan component with immune activity. Background technique [0002] Lentinan is an effective active ingredient extracted from the fruiting bodies of Lentinus edodes, and is the main active substance of Lentinus edodes. A large number of studies have shown that it has significant anti-virus, anti-tumor, immune function regulation and stimulation of interferon formation. Therefore, lentinan has great application value in the field of medicine and health food. my country is a big producer of shiitake mushrooms, and the trade volume of shiitake mushrooms accounts for more than 95% of the world, which provides a rich source for the preparation of shiitake polysaccharides. In view of this, lentinan has been extensively studied in my country and even in the world, and has become an important functional ingredient in health pro...

AI Technical Summary

Problems solved by technology

[0004] There are mainly three problems in the industrial separation of polysaccharides in the prior art: (1) the purity of polysaccharides obtained by separation and purification is low; (2) the technical solutions used are difficult in the actual industrial scale-up process, including high scale-up costs and some equipment cannot be scaled up , Poor safety after amplification, etc.; (3) The technical solutions used are not green and sustainable enough, including high energy consumption, low efficiency, and low utilization rate of raw materials, etc.

Patent CN200610116956.3 discloses a method for purifying lentinan using an improved chromatographic column (DEAE-cellulose as a filler), but this method is eluted with NaOH solution, resulting in subsequent necessary impurity removal steps and in food Field application limitations, therefore, are not conducive to its industrial scale-up

Patent CN201120045307.5 discloses a device for separating, purifying and concentrating lentinan. The device uses ceramic membrane microfiltration (0.05 μm ~ 0.5 μm) and reverse osmosis. The function of the microfiltration in this method is solid-liquid separation, so , its separation effect on lentinan is poor, and it is also difficult to achieve the separation between lentinan fractions

Patent CN201310753452.2 discloses a method for extracting and grading lentinan. The technical solution described in this method involves various organic reagents such as chlorobenzene, calcium chloride, Sevag reagent and acetone; the purification steps of this method are complicated and the efficiency is low , in addition, after treatment by such methods, the recovery and utilization of the remaining components except polysaccharide components is facing great difficulties, which is not green enough and sustainable

[0005] In summary, the higher physiological and pharmacological activities of lentinan are well known to those skilled in the art. However, the existing technology for the industrial extraction, separation and purification of lentinan has problems of "high purity, low efficiency, and difficult industrialization", " High efficiency, industrialization, low purity" and difficult "green, sustainable development" and other issues

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