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Multi-joint bacterium lacticum composition and application thereof to vagina health of females

A composition, the technology of Lactobacillus, applied in the field of microorganisms, can solve the problems of vaginal microecology not restored to a healthy state, microbial resistance, high recurrence rate, etc., and achieve wide coverage, good treatment and prevention, and good probiotic ability Effect

Active Publication Date: 2020-01-07
SICHUAN ANAEROBIC BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although antibiotic treatment is effective, it also has great defects. It has the following two aspects: (1) it has an inhibitory effect on all antibiotic-sensitive microorganisms in the vaginal microenvironment. Therefore, the vaginal microecology has not recovered to a healthy, The balance state that can resist the invasion of pathogenic bacteria, the suppressed or killed pathogenic microorganisms or foreign pathogenic microorganisms will reproduce again or even cause disease, resulting in relapse or new vaginal inflammation; (2) microorganisms develop drug resistance, and antibiotics Unable to balance the vaginal microecological environment, resulting in refractory BV
Therefore, although antibiotic treatment takes effect quickly, the recurrence rate is high, and the recurrence rate within 3 months is as high as 30%.

Method used

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  • Multi-joint bacterium lacticum composition and application thereof to vagina health of females
  • Multi-joint bacterium lacticum composition and application thereof to vagina health of females
  • Multi-joint bacterium lacticum composition and application thereof to vagina health of females

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1: Comparison of lactic acid production capacity of single bacteria and combined lactobacilli.

[0088] Single bacterium: After the lactobacillus is activated, transfer it to the MRS broth medium, and culture it in parallel at 37°C for 48 hours. Use pH 0.5-5.0 test paper to measure and record the pH value of the lactobacillus liquid cultured for 48 hours, so that The strain was subjected to liquid chromatography, the supernatant was diluted 5 times, concentrated sulfuric acid was added for pretreatment, and the sample was filtered with a 0.22um syringe filter before loading.

[0089] Combination bacteria: After activation and transfer of Lactobacillus, select 3 or more strains for mixed culture, 3 parallel cultures, culture at 37°C for 48 hours, centrifuge, dilute the supernatant 5 times, add concentrated sulfuric acid for pretreatment, load the sample Filter with a 0.22um syringe filter. The relevant parameters of liquid chromatography are as follows:

[0090...

Embodiment 2

[0095] Embodiment 2: single bacterium and combination Lactobacillus hydrogen peroxide production ability contrast.

[0096] Single bacterium: After the lactobacillus is activated, use a pipette to draw 2 μL of the bacterial solution, and spot it in a solution containing 0.25 mg / mL 3,3',5,5'-tetramethylbenzidine and 0.01 mg / mL pepper In the MRS agar of root peroxidase, set 2 parallels at the observation time points of 24h, 48h and 72h and place the plates at the same observation time points in the same anaerobic sealed tank, culture at 37°C, and after the corresponding time , take out the corresponding plate, expose it to the air, observe the color reaction after 30min and take pictures and record: Lactobacillus delbrueckii is used as the positive control, the blue depth produced by Lactobacillus delbrueckii is 4 points, and it is the same as that produced by Lactobacillus delbrueckii. The blue color is quite equal to 3 points, the light blue color produced by Lactobacillus Bie...

Embodiment 3

[0098] Example 3: Comparison of the ability of single bacteria and combined Lactobacillus to inhibit Gardnerella vaginalis.

[0099] Single bacteria: after the activation of Lactobacillus, take 0.1mL of the bacterial liquid and mix it with MRS solid medium, pour it into a 6cm plate, and culture it at 37°C for 48 hours after it is completely solidified, take out the plate, and use a hole punch with an inner diameter of 6mm on the agar medium Punch holes on the top to obtain bacteria cakes; after Gardnerella vaginalis is activated and transferred, dilute the Gardnerella bacterial solution to 100 times with anaerobic sterile PBS buffer solution to 100 times, and take 10 times -1 and 10 -2 Mix 0.5mL of the diluent with 5.25mL of BHI solid medium containing 5% horse serum, pour it into a 9cm plate, and after it is completely solidified, place the Lactobacillus cake lightly on the surface of the BHI agar, and put 4 cakes symmetrically in each plate , 2 parallel strains of each stra...

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Abstract

The invention discloses a multi-joint bacterium lacticum composition. The multi-joint bacterium lacticum composition is an advantage bacterial strain selected by a separating screen in the vagina of ahealthy female in China and at least comprises three active components of Lactobacillus johnsonii (Lactobacillus johnsonii), Lactobacillusgasseri (Lactobacillusgasseri), Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), Lactobacillus crispatus ( Lactobacillus crispatus ) and Lactobacillus jensenii (Lactobacillus jensenii ).

Description

technical field [0001] The invention relates to the field of microbes, in particular to a Lactobacillus conjunctival composition and its application in women's vaginal health. Background technique [0002] From the research reports published by the journal Practical Gynecology "Research Progress and Clinical Significance of Vaginal Microecology" and "Research Progress on the Survival Status of Lactobacillus in the Vaginal Microecology" published by the Chinese Journal of Microecology, we can see that, There are more than 300 kinds of microorganisms symbiosis in the human vagina. They restrict each other and check and balance each other to form a dynamic balance. The occurrence of various vaginitis is related to the imbalance of vaginal micro-ecological environment. [0003] The vagina of healthy women of childbearing age is a microenvironment in which Lactobacillus is the dominant flora. They can use the glycogen in the vaginal epithelial cells to grow and produce H 2 o 2 ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P31/04A61P15/02A61L15/46A61L15/44A61L15/36C12R1/225
CPCC12N1/20A61K35/747A61P31/04A61P15/02A61L15/46A61L15/44A61L15/36Y02A50/30C12N1/205C12R2001/225A61K2300/00A23L33/135A61K8/99A61Q19/00A23V2002/00A23V2200/30
Inventor 承磊刘瑶王琼曾婉秋
Owner SICHUAN ANAEROBIC BIOTECHNOLOGY CO LTD
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