Multi-joint bacterium lacticum composition and application thereof to vagina health of females
A composition, the technology of Lactobacillus, applied in the field of microorganisms, can solve the problems of vaginal microecology not restored to a healthy state, microbial resistance, high recurrence rate, etc., and achieve wide coverage, good treatment and prevention, and good probiotic ability Effect
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Embodiment 1
[0087] Example 1: Comparison of lactic acid production capacity of single bacteria and combined lactobacilli.
[0088] Single bacterium: After the lactobacillus is activated, transfer it to the MRS broth medium, and culture it in parallel at 37°C for 48 hours. Use pH 0.5-5.0 test paper to measure and record the pH value of the lactobacillus liquid cultured for 48 hours, so that The strain was subjected to liquid chromatography, the supernatant was diluted 5 times, concentrated sulfuric acid was added for pretreatment, and the sample was filtered with a 0.22um syringe filter before loading.
[0089] Combination bacteria: After activation and transfer of Lactobacillus, select 3 or more strains for mixed culture, 3 parallel cultures, culture at 37°C for 48 hours, centrifuge, dilute the supernatant 5 times, add concentrated sulfuric acid for pretreatment, load the sample Filter with a 0.22um syringe filter. The relevant parameters of liquid chromatography are as follows:
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Embodiment 2
[0095] Embodiment 2: single bacterium and combination Lactobacillus hydrogen peroxide production ability contrast.
[0096] Single bacterium: After the lactobacillus is activated, use a pipette to draw 2 μL of the bacterial solution, and spot it in a solution containing 0.25 mg / mL 3,3',5,5'-tetramethylbenzidine and 0.01 mg / mL pepper In the MRS agar of root peroxidase, set 2 parallels at the observation time points of 24h, 48h and 72h and place the plates at the same observation time points in the same anaerobic sealed tank, culture at 37°C, and after the corresponding time , take out the corresponding plate, expose it to the air, observe the color reaction after 30min and take pictures and record: Lactobacillus delbrueckii is used as the positive control, the blue depth produced by Lactobacillus delbrueckii is 4 points, and it is the same as that produced by Lactobacillus delbrueckii. The blue color is quite equal to 3 points, the light blue color produced by Lactobacillus Bie...
Embodiment 3
[0098] Example 3: Comparison of the ability of single bacteria and combined Lactobacillus to inhibit Gardnerella vaginalis.
[0099] Single bacteria: after the activation of Lactobacillus, take 0.1mL of the bacterial liquid and mix it with MRS solid medium, pour it into a 6cm plate, and culture it at 37°C for 48 hours after it is completely solidified, take out the plate, and use a hole punch with an inner diameter of 6mm on the agar medium Punch holes on the top to obtain bacteria cakes; after Gardnerella vaginalis is activated and transferred, dilute the Gardnerella bacterial solution to 100 times with anaerobic sterile PBS buffer solution to 100 times, and take 10 times -1 and 10 -2 Mix 0.5mL of the diluent with 5.25mL of BHI solid medium containing 5% horse serum, pour it into a 9cm plate, and after it is completely solidified, place the Lactobacillus cake lightly on the surface of the BHI agar, and put 4 cakes symmetrically in each plate , 2 parallel strains of each stra...
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