Method for measuring content of aldosterone in plasma or serum

Abstract

The invention relates to the technical field of analysis and detection, in particular to a method for measuring the content of aldosterone in plasma or serum. The method comprises the following stepsof adding an internal standard into a sample, adding a zinc sulfate solution for protein precipitation, then adding a phosphoric acid solution for dilution, conducting centrifuging, and taking a supernatant; enriching the supernatant by using a solid-phase extraction plate to obtain an enriched sample; and detecting the enriched sample by ultra-high performance liquid chromatography tandem quadrupole mass spectrometry, wherein the condition of ultra-high performance liquid chromatography is that a chromatographic column is a reversed-phase C18 column, a mobile phase A is methanol, a mobile phase B is ultrapure water, and a gradient elution mode is adopted, and the condition of the quadrupole mass spectrometry is that a negative ion mode is adopted and the scanning method is multi-reactionmonitoring (MRM) ion scanning. The detection method does not have matrix interference, shortens the sample pretreatment time, and has high detection sensitivity, high precision and strong specificity.

Description

technical field [0001] The invention relates to the technical field of analysis and detection, in particular to a method for determining the content of aldosterone in blood plasma or serum. Background technique [0002] Aldosterone (ALD) is a steroid hormone synthesized and secreted by the zona glomerular cells of the adrenal cortex and belongs to the mineralocorticoid family. It has three main functions: 1) It binds to the mineralocorticoid receptors in the main cells of the distal tubule and urinary collecting duct, increases the permeability of the luminal membrane to potassium ions and sodium ions, and activates Na at the basal side + / K + pump, increasing the reabsorption of sodium ions and water while excreting more potassium ions into the urine. 2) Stimulate the secretion of hydrogen ions from the urinary collecting duct, regulate the concentration of bicarbonate in the plasma, and maintain the acid-base balance. 3) Act on the central nervous system through the pos...

AI Technical Summary

Problems solved by technology

[0004] At present, immunoassays are commonly used to detect aldosterone in China, but immunoassays generally have problems such as strong cross-reactivity, poor specificity, low sensitivity, and low accuracy, while ID-LC-MS / MS method is currently widely accepted for small molecule quantitative detection Its advantages are high sensitivity, low detection limit, good selectivity, high accuracy and specificity, and it can distinguish compounds with similar structures

However, due to the low content of aldosterone in the human body, many structural analogues, and the complex composition of human blood, the following problems still need to be solved by mass spectrometry: 1) serious matrix interference, poor specificity, and low sensitivity; 2) improve detection efficiency , effectively shorten the sample pretreatment time; 3) The concentration of the compound varies greatly in different populations, especially some clinical samples such as AVS samples require a wide linear range

[0005] In order to reduce matrix interference, improve specificity, sensitivity, etc., the commonly used method is: the sample is subjected to liquid-liquid extraction or liquid-solid extraction and then concentrated by nitrogen blowing to obtain the sample to be tested. The sample is subjected to high performance liquid chromatography, liquid chromatography tandem mass spectrometry, etc. However, this method has the following problems: complex pre-treatment, long processing time (about 2.5 hours for one sample), low throughput, high reagent cost, and high requirements for operators

[0006] The publication number is CN106770824A, and the title of the invention is "A Method for Detecting Trace Aldosterone in Plasma Using a High-Resolution Mass Spectrometer". The method in the patent has a long pretreatment time, requires a large amount of sample and pretreatment reagents, and has a low recovery rate.

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