Application of molybdenum disulfide/graphite nitrogen carbide composites in matrix-assisted laser desorption ionization time-of-flight mass spectrometry detection
A technology of time-of-flight mass spectrometry and molybdenum disulfide, which is applied in the field of mass spectrometry detection, can solve problems that have not yet been seen, and achieve the effects of suppressing or background noise, eliminating background noise, and improving signal-to-noise ratio
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Embodiment 1
[0022] Example 1: Preparation of MoS by existing conventional chemical deposition method 2 / g -C 3 N 4
[0023] 1) g-C 3 N 4 Preparation: Take 10g of urea powder and place it in an alumina crucible with a cover, heat up to 600°C in a muffle furnace at a rate of 2.3°C / min, keep it warm for 4h, and collect the yellow product, which is g-C 3 N 4 ;
[0024] 2) MoS 2 Preparation: Take 300mg of sodium molybdate and 600mg of thiourea in 70mL of deionized water, stir for 1h to fully dissolve, then transfer to a 100mL polytetrafluoroethylene reactor, calcinate at 200°C for 24h, centrifuge, and use for precipitation After washing several times with ionized water and ethanol, dry at 80 °C for 6 h to obtain MoS 2 ;
[0025] 3) MoS 2 / g -C 3 N 4 Preparation: add g-C 3 N 4 and MoS 2 Grind into powder separately, take 300mg g-C 3 N 4 Dissolve the powder in 30mL ethanol, add 15mg MoS 2 powder, magnetic stirring and mixing for 2 hours, and ultrasonication for 2 hours. The obt...
Embodiment 2
[0030] Example 2: MoS 2 / g -C 3 N 4 As a matrix for the detection of five mixed amino acids
[0031] Using Amino Acids as Small Molecules to Evaluate MoS 2 / g -C 3 N 4 The ability to use MALDI-TOF-MS as a matrix to analyze the molecular weight of small molecules, and compare it with CHCA, MoS2, g-C3N4 as a matrix. Figure 5 It is the mass spectrum of five amino acids (alanine, homoserine, histidine, phenylalanine, tryptophan) in positive ion mode. It can be seen from the figure that the MoS 2 / g -C 3 N 4 When used as a matrix, the matrix interference effect is suppressed, the fragment peaks are almost non-existent, and the five analytes show high signal-to-noise ratio (S / N) in the form of [M+Na]+ and [M+K]+, Resolution (Resolution), signal intensity (Intensity), compared with CHCA, there are only three target substance peaks, g-C 3 N 4 Only two substances can be detected, and MoS2 has only one ion peak, and both have strong background interference, which seriously i...
Embodiment 3
[0033] Example 3: MoS 2 / g -C 3 N 4 As a matrix for the detection of quinolone antibiotics
[0034]In order to better verify MoS 2 / g -C 3 N 4 Whether it has a wide range of application capabilities as a matrix, the applicant continued to investigate MoS 2 / g -C 3 N 4 Used as a substrate for the test of the molecular weight of 4 kinds of quinolone antibiotics (lomefloxacin, enrofloxacin, ofloxacin, ciprofloxacin), the results are as follows Figure 7 shown.
[0035] Depend on Figure 7 It can be seen that MoS 2 / g -C 3 N 4 When used as a matrix, it showed a clean matrix background, and the four analytes achieved high signal intensity and signal-to-noise ratio in the form of [M+Na]+ and [M+K]+, and the effect was obviously superior to that of the other three substances CHCA, MoS 2 , g-C 3 N 4 As a matrix, it was shown that MoS 2 / g -C 3 N 4 Can be used as a MALDI matrix for base pair molecular weight testing.
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