Anti-Escherichia coli Bacillus alvei strain and its antiseptic application in cosmetics

A technology of bacillus and Escherichia coli, which is applied in the field of antiseptic application, to achieve the effect of guaranteeing the quality of cosmetics, wide antibacterial spectrum and strong development potential

Active Publication Date: 2021-05-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The policy stipulates that Bacillus cultures can be used as raw materials to be added to cosmetics, but so far, there are no reports on the application of Bacillus cells or metabolites to cosmetic antiseptics

Method used

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  • Anti-Escherichia coli Bacillus alvei strain and its antiseptic application in cosmetics
  • Anti-Escherichia coli Bacillus alvei strain and its antiseptic application in cosmetics
  • Anti-Escherichia coli Bacillus alvei strain and its antiseptic application in cosmetics

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Determination of Bacillus strains of the present invention and their secondary metabolites to Escherichia coli antibacterial effect

[0028] 1. Experimental method

[0029] Use a disposable polyethylene culture dish, add LB medium, and cool down. The blank group was coated with Escherichia coli at an appropriate concentration, and a filter paper sheet was placed on a designated area on the medium, and sterile water was added to the filter paper sheet; the experimental group was coated with an appropriate concentration of Escherichia coli, and a filter paper sheet was placed on a designated area on the medium. A certain concentration of sterilized Bacillus thallus and its supernatant crude purification are added to the tablet.

[0030] After the inoculation was completed and the double-layer sealing film was sealed, it was cultured upside down in a 37°C incubator for one week, and the diameter of the inhibition zone of each group of cultures against Escher...

Embodiment 2

[0039] Embodiment 2: simulated cosmetics storage test

[0040] 1. Experimental materials, reagents and instruments

[0041] Experimental materials: moisturizing skin care water without preservatives (ingredients: glycerin, vitamin C, vitamin E, rose hydrosol: linalool, eugenol, rose ether), Escherichia coli, Bacillus numbered YM37, normal saline, Sterile water, centrifuge tubes, bamboo sticks, sealed bottles with blue caps, etc.

[0042] Experimental reagents: LB liquid, solid medium, Casson.

[0043] Experimental equipment: shaker, oven, ultra-clean table, etc.

[0044] 2. Experimental method

[0045] (1) Preparation of Bacillus somatic cell disruptor

[0046] Scrape a small amount of Bacillus YM37 from the plate and inoculate it into an Erlenmeyer flask containing 50 mL of liquid LB medium, and culture it overnight in a shaker at 37°C. Take 1 mL of the Bacillus suspension after overnight cultivation and add it to a conical flask filled with 50 mL of LB liquid medium, pu...

Embodiment 3

[0058] Embodiment 3: the species identification of bacillus bacterial strain described in the present invention

[0059] 1. Experimental method

[0060] According to the pClone007 Vector Kit (TSV-007) step, the total DNA of the bacterial strain of the present invention was extracted, the 16S rDNA gene of the bacteria was amplified with primers M13F-47 and M13R-48, and the amplified product was recovered for sequencing. The sequence of 16S rDNA was used to identify the species. The obtained sequence results were compared by Blast at NCBI, and the recognized standard sequence data homologous to the 16S rDNA of the strain was obtained from the genBank database, and the sequence similarity was calculated using MEGA software and Neighbor-Joining was used as the system developmental analysis.

[0061] 2. Implementation result analysis

[0062] The strain of the present invention is identified through the determination of the full sequence of 16S rDNA, and its sequence result is s...

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Abstract

The invention discloses an anti-escherichia coli bacillus alvei strain and its antiseptic application in cosmetics. The strain is classified as Paenibacillus alvei YM37, and is preserved in the China General Biological Culture Collection and Management Center with the preservation number CGMCC No.18238. The invention also provides a method for preparing the bacillus culture solution and its metabolites. The Paenibacillus alvei and its secondary metabolites described in the present invention can well inhibit the growth of Escherichia coli in cosmetics, are suitable for cosmetics and skin care products with various textures, have good application prospects, strong development potential, and have It has the characteristics of high efficiency, energy saving and environmental friendliness, and can be applied to the preparation of cosmetic preservatives.

Description

technical field [0001] The invention belongs to the field of new strain screening technology and microorganism technology, and particularly relates to an anti-Escherichia coli bacillus alvei strain and its antiseptic application in cosmetics. Background technique [0002] Cosmetics and skin care products have become one of the necessities of women all over the world. However, because the composition and texture of cosmetics are suitable for the growth of microorganisms, various chemically synthesized preservatives are often added, and these preservatives often cause users to have redness, swelling, itching and other allergies , Abnormal symptoms. [0003] At present, in order to inhibit the corruption of cosmetics at home and abroad, paraben and phenoxyethanol are often added as preservatives. As of 2014 (data published in January 2016), paraben remains the most commonly used cosmetic preservative in the U.S. The frequency of use of preservatives exceeds 77% (Liang Chenyu....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K8/99A61Q19/00C12R1/01
CPCA61K8/99A61K2800/524A61K2800/85A61Q19/00C12N1/20C12N1/205C12R2001/01
Inventor 周文文俞笑梅竺莹莹汤曦
Owner ZHEJIANG UNIV
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