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Paenibacillus mucilaginosus and application thereof

A bacillus and jelly-like technology, applied in the field of microbial screening, can solve problems such as ineffective inhibition of botrytis cinerea, impact on health and ecology, resistance of tomatoes and strawberries, etc., to increase soil fertility and permeability , Promote growth, improve the effect of fruit setting rate

Inactive Publication Date: 2020-04-10
山东蔚蓝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term use of chemical pesticides will cause resistance to tomatoes and strawberries, and the long-term use of chemical pesticides in large quantities will have a long-term impact on people's health and ecology
Although the currently disclosed jelly-like Paenibacillus has a certain effect of inhibiting the growth of pathogenic bacteria, the inhibitory effect on Botrytis cinerea is not obvious

Method used

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  • Paenibacillus mucilaginosus and application thereof
  • Paenibacillus mucilaginosus and application thereof
  • Paenibacillus mucilaginosus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Separation and screening of Jelly-like Paenibacillus WL-72

[0023] 1) Silicate bacteria solid culture medium: sucrose 5g, Na 2 HPO 4 2g, MgSO 4 ·7H 2 O 0.5g, FeCl 3 0.005g, CaCO 3 0.1g, 0.5g soil minerals, 10-20g agar, 1000ml water, pH 7.0-7.5.

[0024] 2) Preparation of soil diluent: This soil sample was taken from a greenhouse in Huimin County, and 20 samples were sampled. Weigh 10 grams of each sample into 100ml sterile water, shake for 30 minutes, and make a concentration of 10 -1 Soil diluent.

[0025] 3) Bacterial separation: Dilute the prepared soil dilution in a 10-fold gradient, and then apply a suitable gradient of solution on the silicate bacterial solid medium, spread each sample on three plates, and incubate at 30°C for 30 hour. After the cultivation, one of the bacteria with large colonies and fast growth on the plate was selected as the experimental strain and named as Bacillus jelly-like WL-72.

Embodiment 2

[0026] Example 2: Identification of the genus of strain WL-72

[0027] 1) The morphological characteristics of strain WL-72: The colony on the solid medium of silicate bacteria is round, colorless, transparent, with neat edges, smooth, viscous, elastic and easy to stretch into silk. Under the microscope, the bacteria are rod-shaped with thick capsules, Gram staining is negative, and the spores are terminal, oval and large. The strain does not grow in common nutrient agar medium and broth medium. The protease activity test was positive, the glucose fermentation test was negative, and the mannitol fermentation test was negative.

[0028] 2) Physiological and biochemical characteristics: citric acid utilization (-), indole production (-), urinary enzyme (-), mannitol (-), rhamnose (-), etc.

[0029] 3) Molecular genetic classification and identification: The 16s rRNA gene sequence of strain WL-72 was determined, and the sequence comparison analysis showed that it was compared with man...

Embodiment 3

[0031] Example 3: Preparation of Bacillus jelly-like WL-72 powder

[0032] 1) Seed medium: glucose 10g / L, K 2 HPO 4 2g / L, MgSO 4 ·7H 2 O 1.5g / L, FeCl 3 0.005g / L, CaCO 3 2g / L, beef extract 0.5g / L, initial pH 7.0.

[0033] 2) Fermentation medium: glucose 30g / L, K 2 HPO 4 2g / L, MgSO 4 ·7H 2 O 2g / L, beef extract 5g / L, initial pH 7.0.

[0034] 3) Activation and expansion: Activate Bacillus glacius on a solid medium, and then select a single colony on the solid medium, insert it into 100ml seed medium, culture at 30°C, 200rpm for 24 hours, and expand it. As a seed liquid.

[0035] 4) Fermentation culture: Transfer the cultured seed liquid to the fermentation medium at 5% inoculum, and continue culturing at 30°C. When the spore rate reaches over 90%, the culture is terminated.

[0036] 5) Collecting and milling: Centrifuge at 5000rpm for 10min in a centrifuge to remove the fermentation supernatant, collect the bacterial sludge, then add an appropriate amount of carrier corn starch, stir we...

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PUM

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Abstract

The invention belongs to the technical field of microorganism screening application, and particularly relates to paenibacillus mucilaginosus and application thereof. The paenibacillus mucilaginosus WL-72 screened by the invention can be prepared into a microbial inoculum and is applied to greenhouse crop planting. The Bacillus powder is applied to tomato planting, so the soluble solid content of tomatoes and the yield per mu can be increased, and gray mold is greatly reduced; when the Bacillus powder is applied to strawberry planting, the average single fruit weight of strawberries can be increased, the plant yield is increased, and gray mold is greatly reduced. In addition, the Bacillus powder can change the soil flora structure, the soil fertility and permeability are improved, and the Bacillus powder plays an important role in soil improvement.

Description

Technical field [0001] The invention belongs to the technical field of microbial screening applications, and specifically relates to a jelly-like bacillus strain and its application. Background technique [0002] At present, for increasing the yield of greenhouse crops, preventing soil salinization, hardening and other problems, the commonly used solutions mainly include irrigation and washing of salt, deep ploughing, soil replacement, and mixing of sand, but these methods often treat the symptoms rather than the root cause. After a period of cultivation, the soil often reappears before the damage. In recent years, the use of compound microbial agents has become an ideal solution. Microbial compound inoculants are generally composed of Bacillus subtilis, Gliocladium spores, Bacillus licheniformis and other bacteria, which have the functions of improving soil structure, transforming soil nutrients, reducing crop diseases and insect pests, promoting crop growth, and increasing cro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/80C05G3/60C12R1/07
CPCC05F11/00C05G3/00C12N1/20C12N1/205C12R2001/07
Inventor 牛庆奎梁莉魏焕巍孔垂彬罗福玖郭宝帅张娜周英俊
Owner 山东蔚蓝生物科技有限公司
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