Method for separating carnosine and histidine

Abstract

The invention discloses a carnosine and histidine separation method which comprises the following steps: adding a mixture containing carnosine and histidine into an aqueous two-phase system for extraction to realize separation of carnosine and histidine; wherein the aqueous two-phase system comprises a heavy phase serving as an extraction agent and a light phase serving as a detergent, the heavy phase consists of inorganic salt and water, and the light phase consists of a polymer and water or an organic solvent and water; the inorganic salt is at least one of sulfate, phosphate, carbonate andchloride; the polymer is at least one of polyethylene glycol, polypropylene glycol, polyvinyl pyrrolidone and an ethylene oxide-propylene oxide copolymer; wherein the organic solvent is at least one of low-carbon alcohol with the carbon number of 1-5, acetone and acetonitrile. According to the method, an aqueous two-phase system is used as a separation medium, efficient separation of carnosine andhistidine is achieved, the operation process is simple, and amplification is easy.

Description

technical field [0001] The invention relates to the technical field of chemical separation, in particular to a method for separating carnosine and histidine. Background technique [0002] Carnosine was discovered in innervated tissues of vertebrates as early as 1900. The scientific name of carnosine is β-alanyl-L-histidine, which is an endogenous dipeptide composed of β-alanine and L-histidine, which can participate in regulating various physiological activities of the human body, and has excellent natural Anti-oxidation, anti-free radicals, anti-aging, nerve protection, vasodilation, and fatigue-relieving effects have been used in medicine, health care, food, cosmetics and other industries. [0003] At present, most of the synthesis methods of carnosine use β-alanine or β-alanine precursor and L-histidine as starting materials, and synthesize by protecting the amino group, activating the carboxyl group, and selecting a suitable protecting agent and activating agent. ; or ...

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Problems solved by technology

[0004] However, the existing synthetic methods all have the problem of difficult separation, especially carnosine and histidine are similar in nature and difficult to separate, so it is difficult to obtain high-purity carnosine

[0005] There are very few reports on the separation of carnosine and histidine in the existing literature. According to the literature, the separation between other amino acids, amino acids and polypeptides mainly uses the ion exchange method, and the mixed solution is deacidified or neutralized until the pH is reduced to the concentration of amino acids and polypeptides. After desalination below the electric point, most of the basic amino acids and some neutral amino acids are adsorbed with cation exchange resin, and single amino acids and peptides are obtained by gradient elution

But because the isoelectric point of histidine is 7.6, the isoelectric point of carnosine is 7.0, and the isoelectric point of the two is very close, causes cross-elution to be relatively serious, and the purity of carnosine is low, the yield is low, greatly reduced the whole process separation effect of the process

[0006] It can be seen that there is no report on the separation method of histidine and carnosine suitable for industrial application, and the separation technology of carnosine and histidine needs to be improved urgently

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