Method for separating carnosine and histidine

A separation method and technology for histidine, applied in the field of separation of carnosine and histidine, can solve the problems of low yield, low purity of carnosine, difficult separation, etc., and achieve reduced production cost and energy consumption, high selective separation capability , the effect of simplifying the separation step

Active Publication Date: 2020-09-01
QUZHOU RES INST OF ZHEJIANG UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing synthetic methods all have the problem of difficult separation, especially carnosine and histidine are similar in nature and difficult to separate, so it is difficult to obtain high-purity carnosine
[0005] There are very few reports on the separation of carnosine and histidine in the existing literature. According to the literature, the separation between other amino acids, amino acids and polypeptides mainly uses the ion exchange method, and the mixed solution is deacidified or neutralized until the pH is reduced to the concentration of amino acids and polypeptides. After desalination below the electric point, most of the basic amino acid

Method used

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  • Method for separating carnosine and histidine

Examples

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Effect test

Embodiment 1

[0049] Prepare a two-phase aqueous system consisting of polyethylene glycol 400, ammonium sulfate, and water, wherein the volume ratio of polyethylene glycol 400 to water is 3:10, and the mass ratio of ammonium sulfate to water is 1:2. After equilibrium, the light phase is polyethylene glycol + water phase, and the heavy phase is ammonium sulfate + water phase, and the light and heavy phases are taken out for later use. A mixture of raw materials carnosine and histidine (carnosine / histidine=18.77%, mass ratio) was dissolved in the light phase to prepare a raw material solution. The heavy phase is used as the extractant, the light phase is used as the detergent, and the flow ratio of the extractant, raw material liquid, and detergent is 2:0.6:7. Fractional extraction is carried out at 25°C. There are 20 stages in the extraction section, and the washing section A total of 20 stages, collecting the extract and raffinate. The purity of carnosine in the extract was 98.37%, and the...

Embodiment 2

[0051] According to the mass ratio of polyethylene glycol 2000: ammonium sulfate: water = 22.54:50:100, the two-phase aqueous system was prepared and pre-balanced. The light phase was polyethylene glycol + water phase, and the heavy phase was ammonium sulfate + water phase. Light and heavy two-phase standby. A mixture of raw materials carnosine and histidine (carnosine / histidine=18.77%, mass ratio) was dissolved in the light phase to prepare a raw material solution. The heavy phase is used as the extractant, the light phase is used as the detergent, and the flow ratio of the extractant, raw material liquid, and detergent is 1:1:9. Fractional extraction is carried out at 25°C. There are 7 stages in the extraction section, and the washing section There are 16 stages in total, and the extract and raffinate are collected. The purity of carnosine in the extract was 99.11%, and the yield was 91.69%; the purity of histidine in the raffinate was 98.46%, and the yield was 99.85%.

Embodiment 3

[0053] Prepare a two-phase aqueous system composed of polyethylene glycol 400, dipotassium hydrogen phosphate, water, wherein the volume ratio of polyethylene glycol 400 and water is 3:10, and the mass ratio of dipotassium hydrogen phosphate and water is 3: 10. After pre-equilibration, the light phase is polyethylene glycol + water phase, and the heavy phase is dipotassium hydrogen phosphate + water phase. Take out the light and heavy phases for later use. A mixture of raw materials carnosine and histidine (carnosine / histidine=18.77%, mass ratio) was dissolved in the light phase to prepare a raw material solution. The heavy phase is used as the extractant, the light phase is used as the detergent, and the flow ratio of the extractant, raw material liquid, and detergent is 1:0.01:0.98. Fractional extraction is carried out at 25°C. There are 30 stages in the extraction section, and the washing section There are 30 stages in total, and the extract and raffinate are collected. Th...

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Abstract

The invention discloses a carnosine and histidine separation method which comprises the following steps: adding a mixture containing carnosine and histidine into an aqueous two-phase system for extraction to realize separation of carnosine and histidine; wherein the aqueous two-phase system comprises a heavy phase serving as an extraction agent and a light phase serving as a detergent, the heavy phase consists of inorganic salt and water, and the light phase consists of a polymer and water or an organic solvent and water; the inorganic salt is at least one of sulfate, phosphate, carbonate andchloride; the polymer is at least one of polyethylene glycol, polypropylene glycol, polyvinyl pyrrolidone and an ethylene oxide-propylene oxide copolymer; wherein the organic solvent is at least one of low-carbon alcohol with the carbon number of 1-5, acetone and acetonitrile. According to the method, an aqueous two-phase system is used as a separation medium, efficient separation of carnosine andhistidine is achieved, the operation process is simple, and amplification is easy.

Description

technical field [0001] The invention relates to the technical field of chemical separation, in particular to a method for separating carnosine and histidine. Background technique [0002] Carnosine was discovered in innervated tissues of vertebrates as early as 1900. The scientific name of carnosine is β-alanyl-L-histidine, which is an endogenous dipeptide composed of β-alanine and L-histidine, which can participate in regulating various physiological activities of the human body, and has excellent natural Anti-oxidation, anti-free radicals, anti-aging, nerve protection, vasodilation, and fatigue-relieving effects have been used in medicine, health care, food, cosmetics and other industries. [0003] At present, most of the synthesis methods of carnosine use β-alanine or β-alanine precursor and L-histidine as starting materials, and synthesize by protecting the amino group, activating the carboxyl group, and selecting a suitable protecting agent and activating agent. ; or ...

Claims

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Application Information

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IPC IPC(8): C07D233/64
CPCC07D233/64
Inventor 邢华斌郭阳崔希利
Owner QUZHOU RES INST OF ZHEJIANG UNIV
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