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Pediococcus acidilactici strain and application thereof in aquaculture

A technology of Pediococcus lactis and aquaculture, applied to Pediococcus lactis and its application in aquaculture, can solve problems such as damage to normal flora structure, drug resistance of pathogenic bacteria, endangering human health, etc., and achieve the goal of improving environmental water quality. Effect

Active Publication Date: 2020-09-18
青岛玛斯特生物技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive use of antibiotics will not only damage the normal flora structure of the California perch intestine, but also easily lead to drug resistance of pathogenic bacteria. Drug-resistant strains enter the human body through water or fish, eventually endangering human health and destroying the surrounding water environment. micro-ecological balance, these phenomena have aroused great concern

Method used

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  • Pediococcus acidilactici strain and application thereof in aquaculture
  • Pediococcus acidilactici strain and application thereof in aquaculture
  • Pediococcus acidilactici strain and application thereof in aquaculture

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1: Isolation, screening and identification of strains

[0024] 1. Sample:

[0025] The intestines of live California perch were collected from a market in Jiaozhou City, Qingdao. Healthy and vigorous California perch intestines were extracted by aseptic operation for subsequent separation.

[0026] 2. Screening method

[0027] (1) Enrichment culture

[0028] Take the intestinal sample and put it into a grinder, put it in normal saline, homogenize it, and centrifuge at a low speed, so that the bacteria in the sample are fully dispersed in the upper layer of normal saline, and make a 10-fold gradient dilution. The above bacterial suspension was transferred to a liquid MRS medium with a 10% inoculum amount, and cultured at 30°C for 24h-48h to obtain an enriched bacterial suspension.

[0029] (2) Separation

[0030] Apply 0.2ml of bacterial suspension on DM solid medium plate by 10-fold dilution method, and cultivate in a 30°C incubator; when a single colony ...

Embodiment 2

[0043] Application of embodiment 2 Pediococcus lactis MST02 in purifying aquaculture water

[0044] 1. Preparation of bacteria powder

[0045] Activate Pediococcus lactis MST02, expand the culture, and undergo liquid fermentation, centrifugation, concentration, and freeze-drying to make a live bacteria content of about 10 10 CFU / g of bacteria powder.

[0046] 2. Experimental location

[0047] A California perch breeding factory in Yancheng, Jiangsu.

[0048] 3. Experimental process

[0049] Six ponds with similar size and water quality were selected, three in each of the control group and the experimental group were paralleled, and the nitrite nitrogen content in each water body was detected respectively. Then add 10ppm of Pediococcus lactis powder to the water body of the experimental group, and do not add it to the control group. The water temperature in the experiment was 28-30°C, and the same equipment was used to continuously aerate the water body during the experiment...

Embodiment 3

[0054] Embodiment 3 Pediococcus lactis MST02 stomach acid resistance test

[0055] The simulated gastric juice components are: tryptone 8.5g, glucose 3.5g, sodium chloride 2.05g, calcium chloride 0.13g, potassium chloride 0.42g, potassium dihydrogen phosphate 0.55g, ox bile salt 0.05g, lysozyme 0.11g, Pepsin 13.5g, distilled water 1000ml, hydrochloric acid to adjust the pH to 1.5 and 2.5 respectively, and sterilize at 115°C for 20 minutes.

[0056] Another strain of Pediococcus lactis AS1.2696 was selected as the experimental control group from the Institute of Microbiology, Chinese Academy of Sciences.

[0057] Activate Pediococcus lactis MST02 and Pediococcus lactis AS1.2696 under the same conditions, then transfer it into 100ml MRS liquid medium according to the inoculum size of 0.6%, culture at 30°C for 24 hours, and add the two bacterial solutions to the prepared aseptic mock Gastric juice, after 0h and 2h, the number of viable bacteria was detected by plate counting met...

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Abstract

The invention relates to the technical field of functional microorganism screening and application, and particularly provides a novel pediococcus acidilactici strain and an application thereof in aquaculture. The pediococcus acidilactici is screened from the intestinal tract of micropterus salmoides, has the preservation number of CCTCC NO: M2020094, can significantly improve the surrounding waterquality of aquatic animals, is resistant to the acidic environment of the stomach of the aquatic animals, has high stability, improves the immunity and disease resistance of the aquatic animals, promotes the growth of the aquatic animals, and can be widely applied to the field of aquatic feeds.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a strain of Pediococcus lactis and its application in aquaculture. Background technique [0002] With the increase in demand for aquatic products and the international development of aquatic product trade, the aquaculture industry has developed by leaps and bounds in recent years. However, the intensification and high-density scale of aquaculture is expanding day by day, the deterioration of the aquaculture environment and the harm of diseases have brought huge economic losses to the aquaculture industry. [0003] California sea bass (Micropterus salmoides) is a carnivorous fish with delicious meat, no intermuscular spines, fast growth, easy to catch, and wide temperature adaptability. It occupies a large proportion in my country's aquaculture industry. As time goes by, the problem of California perch farming diseases has become more and more apparent....

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18A23K50/80A23K10/18C02F3/34C12R1/01C12R1/10C12R1/125C12R1/865C12R1/23C02F103/20
CPCC12N1/20C12N1/18A23K50/80A23K10/18C02F3/34C02F3/347C02F2103/20C12R2001/01C12N1/205
Inventor 郭本月刘广倪梦丽魏万权周怡王磊
Owner 青岛玛斯特生物技术有限公司
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