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Preparation method of a layered double hydroxide nanosheet-copper sulfide quantum dot heterogeneous nanocomposite

A nanocomposite and hydroxide technology, applied in the field of medical materials, can solve problems such as no anti-cancer effect, and achieve the effect of improving production efficiency and utilization rate

Active Publication Date: 2022-06-07
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, LDH nanosheets have no anti-cancer effect by themselves, and need to cooperate with other drugs or materials to exert their effect

Method used

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  • Preparation method of a layered double hydroxide nanosheet-copper sulfide quantum dot heterogeneous nanocomposite
  • Preparation method of a layered double hydroxide nanosheet-copper sulfide quantum dot heterogeneous nanocomposite
  • Preparation method of a layered double hydroxide nanosheet-copper sulfide quantum dot heterogeneous nanocomposite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) 16.0mmol sodium hydroxide was dissolved in 37.5mL deionized water to obtain sodium hydroxide solution;

[0037] (2) 1.7mmol aluminum nitrate and 5.1mmol magnesium nitrate are dissolved in 12.5mL deionized water, obtain metal salt solution;

[0038] (3) Under a nitrogen atmosphere, slowly drop the sodium hydroxide solution into the metal salt solution, stir vigorously for 1 h at room temperature, then centrifuge at 12,000 rpm for 15 min, and wash with water 3 times respectively to collect the layered double hydroxide Crude nanosheets;

[0039] (4) The crude product of layered double hydroxide nanosheets was dispersed in 40 mL of deionized water, placed in a hydrothermal reaction kettle, and placed in a 120° C. oven for 8 hours of hydrothermal treatment to obtain layered double hydroxide nanosheets.

[0040] (5) Dissolve 7.25 mg of the prepared layered double hydroxide nanosheets with 6.8 mg of copper chloride and 27 mg of polyvinylpyrrolidone in 9 mL of deionized wa...

Embodiment 2

[0044] Take 2 mg of the layered double hydroxide-copper sulfide quantum dot nanocomposite prepared in Example 1, disperse it in 2 mL of water and place it in a cuvette, with power densities of 0.5, 1.0, 1.5, and 2.0W / cm 2 The solution was irradiated with an 808 nm near-infrared laser, and the temperature of the solution was monitored over time. figure 2 It can be seen that the obtained nanocomposite undergoes obvious photothermal conversion under illumination, and the temperature of the solution increases continuously with the prolongation of illumination time.

Embodiment 3

[0046] The layered double hydroxide-copper sulfide quantum dot nanocomposites prepared in Example 1 were used for intracellular lysosome localization experiments.

[0047] (1) The layered double hydroxide-copper sulfide quantum dot nanocomposite prepared in Example 1 was stirred in an aqueous solution of an equal amount of green fluorescent marker (FITC) for 4 h. Human breast cancer cells 4T1 were seeded in 12-well cell culture plates, 1 × 10 per well. 5 1 mL of DMEM medium was added to the medium of each well. After the cells were cultured for 24 hours, the original medium was removed, and 500 μL of nanocomplexes containing 10 μg / mL of FITC labeled with FITC were added to the experimental group, and the culture was continued for 4 hours;

[0048](2) Aspirate the supernatant in the well plate, rinse the cells with phosphate buffer solution, and label the cell lysosomes with the lysosome probe LysoTracker Rad, observe and take pictures under a laser confocal microscope.

[004...

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Abstract

The invention discloses a method for preparing a layered double hydroxide nanosheet-copper sulfide quantum dot heterogeneous nanocomposite. The in-situ growth method is used to grow copper sulfide quantum dots on the layered double hydroxide nanosheet. Utilizing polymer confinement, CuS independently dispersed on LDH without agglomeration. The layered double hydroxide nanosheet-copper sulfide quantum dot heterogeneous nanocomposite prepared by the present invention has photothermal properties on the one hand, and can convert heat energy after near-infrared irradiation to exert the effect of photothermal treatment of tumors; on the other hand, the The formed metal-semiconductor heterostructure helps copper sulfide to separate electrons and holes under near-infrared light, thereby more efficiently catalyzing the generation of biotoxic active oxygen in tumor cells and killing tumor cells.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and in particular relates to a preparation method of a layered double hydroxide nanosheet-copper sulfide quantum dot heterogeneous nanocomposite. Background technique [0002] With the development of tumor therapy, nanomaterials possess superior morphological, physicochemical, and functional properties, and various therapeutic strategies have undergone extensive changes. Various active targeting strategies targeting specific organelles have attracted increasing attention in recent years. In particular, the lysosome is the first organelle to transport nanomaterials after endocytosis. Compared with normal cells, lysosomes in cancer cells are overdeveloped, large in size, large in number, and high in cathepsin activity, showing the potential of lysosomes as targeted organelles to improve selectivity and significant efficacy in tumor therapy. [0003] Copper sulfide (CuS) has been widely ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K49/22A61P35/00B82Y30/00B82Y40/00C01F7/00C01G3/12
CPCA61K41/0052A61K41/0057A61K49/222A61P35/00C01F7/00C01G3/12B82Y30/00B82Y40/00C01P2004/04C01P2004/20C01P2004/10A61K2300/00
Inventor 陈爱政刘晨光王士斌
Owner HUAQIAO UNIVERSITY
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