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Biological nanometer magnetite, and preparation method and application thereof

A bio-nano, magnetite technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of thermodynamic instability, development and application limitations, reduction in reducing ability, etc. The effect of eliminating efficiency and no secondary pollution

Active Publication Date: 2020-11-06
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Although nano-materials such as nano-zero-valent iron have high removal efficiency for pollutants, the nano-materials synthesized by chemical means usually have a particle size of 50-100 nm, and are thermodynamically unstable, easy to agglomerate and easily oxidized and corroded, thereby causing reduction. The decline in ability limits its development and application
[0005] Therefore, it is necessary to improve the classic nanomaterials to overcome the problems of weak stability, easy precipitation and reduced reduction ability, so as to provide more favorable conditions for the reduction and degradation of organochlorine pesticides by nanomaterials.

Method used

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  • Biological nanometer magnetite, and preparation method and application thereof
  • Biological nanometer magnetite, and preparation method and application thereof
  • Biological nanometer magnetite, and preparation method and application thereof

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Experimental program
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Embodiment 1

[0053] Enrichment and purification culture of embodiment 1 iron-reducing bacteria

[0054] Streak the Shewanella onesidensis MR-1 (purchased from the China Marine Microorganism Culture Collection Management Center) preserved at -80°C on an LB plate, and cultivate overnight at 30°C; take a single colony and put it into the liquid In LB medium, cultivate at 30°C for 16h; transfer to new liquid LB medium at 1% (v / v) inoculum size, and culture at 30°C at 180rpm for 16h. Centrifuge the cultured bacterial solution at 5000rpm for 5min, wash and centrifuge with sterile water, repeat 3 times, and finally resuspend the bacterial suspension to the required concentration (10 10 cells / mL) for later use.

[0055] Among them, the preparation of LB liquid culture medium is to add tryptone (tryptone) 10g, yeast extract (yeast extract) 5g, NaCl 10g in 950mL deionized water, shake the container until the solute is dissolved; dilute to 1L with deionized water, 121 ℃ autoclave for 20min. When p...

Embodiment 2

[0056] The synthesis of embodiment 2 ferrihydrite

[0057] First prepare 0.4mol / L FeCl 3 .6H 2 O solution and 1mol / L NaOH solution; in the case of vigorous and uniform stirring, NaOH solution was added dropwise at a constant speed (0.5mL / s) to the FeCl contained in a high-density polyethylene container. 3 Solution, when the pH is close to neutral, the dropping speed should be slowed down until the pH reaches 7.0 and a thick dark red ferrihydrite suspension is formed; then the suspension is allowed to age for 4-6 hours, and the pH will be slightly lower at this time. Decrease (<1pH); after re-adjusting the pH to 7.0, centrifuge the suspension at 7000rpm and 4°C for 10min, pour off the supernatant, add sterilized ultrapure water and stir to resuspend evenly, then repeat the centrifugation for 7 After about two times until the concentration of chloride ion in the ferrihydrite suspension is lower than 1mmol / L, the ferrihydrite whose iron concentration is 200mmol / L is obtained. ...

Embodiment 3

[0058] The preparation of embodiment 3 bionano magnetite

[0059] Get the ferrihydrite that 25mL embodiment 2 obtains and place in the anaerobic bottle of 250mL, the bacterium suspension that obtains in 2mL embodiment 1 is inoculated in ferrihydrite, and adding final concentration is 20mmol / L lactic acid as required by microorganism Electron donor, while using a final concentration of 25mmol / L sodium bicarbonate buffer to adjust the pH value of the system to 7.0, the final volume of the reaction system obtained is 200mL, the final concentration of iron in ferrihydrite is 25mmol / L, and N 2 / CO 2 The mixed gas was deoxygenated for 30 minutes, and kept in a constant temperature anaerobic box at 25°C for 7 to 8 days in the dark to obtain a black suspension; the suspension was separated by centrifugation (10000rpm, 15min), the supernatant was removed, and reserved Black precipitate; wash the magnetite precipitate repeatedly with ultrapure water three times, centrifuge (10000rpm, 1...

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Abstract

The invention discloses biological nanometer magnetite, and a preparation method and application thereof. The biological nanometer magnetite is prepared from iron reducing bacteria and iron oxide. Thepreparation method comprises the following steps: inoculating iron reducing bacteria into iron oxide, adding an electron donor, adjusting a pH value, removing oxygen, and conducting culturing to obtain the biological nanometer magnetite. The preparation method further comprises the following steps: centrifuging the obtained biological nanometer magnetite, then conducting cleaning with water and absolute ethyl alcohol in sequence, and successively carrying out separating and drying. The biological nanometer magnetite provided by the invention is small in particle size and rich in ferrous iron,and has high adsorption characteristic and reducibility. Compared with a traditional bioremediation method, when the biological nanometer magnetite provided by the invention is used for repairing, the toxicity of an organochlorine concentration to microorganisms is prevented, the biological nanometer magnetite has the advantages of capability of treating high-concentration organochlorine pesticides, high elimination efficiency and the like, the elimination rate of pentachlorophenol, with a concentration as high as 10 mg / Kg, in soil is as high as 91.56%, and secondary pollution is avoided.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a biological nano-magnetite and its preparation method and application. Background technique [0002] Organochlorine pesticides are highly toxic and refractory persistent organic pollutants ubiquitous in nature. In agricultural production, it is widely used in insecticide, sterilization, wood preservation, etc., which has caused serious pollution to wetlands, water bodies, soil, etc., and the soil is the "gathering place" of organochlorine pesticides. The organochlorine pesticides in the soil will seriously endanger the safety of agricultural products after being absorbed by crops, and enter the human body through the food chain, endangering human health. How to efficiently eliminate pentachlorophenol pollution in soil has become an environmental problem to be solved urgently. [0003] At present, there are physical methods, chemical methods and bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N1/20B09C1/10C02F3/34C12R1/15C12R1/01C02F101/36
CPCC12N11/14C12N1/20B09C1/10C02F3/34C02F2101/36
Inventor 陈曼佳刘承帅乔江涛童辉周继梅吕亚辉
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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