Method for synergistically fermenting low-salt fermented bean curds by multifunctional bacteria and application
A collaborative fermentation and multi-functional technology, applied in the direction of microorganism-based methods, applications, biochemical equipment and methods, etc., can solve the problems of high salt content and single flavor of the product, and achieve the reduction of salt content, increase of flavor components, and outstanding floral fragrance Effect
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Embodiment 1
[0027] The preparation method of embodiment 1 low-salt fermented bean curd, comprises the following steps.
[0028] (1) Domestication and improvement of Mucor radiata and Aspergillus oryzae
[0029] Add L-tyrosine to the PDA agar medium (gradually increase the L-tyrosine concentration in the range of 0-0.04%), inoculate Mucor actinosa and Aspergillus oryzae, and carry out domestication by repeated passage for 15 generations. The activity of phthaloyl tyrosine hydrolase in Mucorra jazhi and Aspergillus oryzae decreased significantly, and their tyrosine-producing ability decreased by 34.3% and 32.5% respectively compared with unaccustomed strains, and the white spot rate of fermented bean curd decreased.
[0030] (2) Preparation of starter
[0031] The preparation process of the mold spore suspension of the described Monascus purple, domesticated radiomucor yazhi, and domesticated Aspergillus oryzae is as follows: inoculate the three kinds of molds on the PDA plate medium respe...
Embodiment 2
[0048] Example 2 Antibacterial activity of bacteriocin produced by Enterococcus faecalis and its application in fermented bean curd
[0049] Enterococcus faecalis (E.Faecium) CGMCC No.16677 used in the present invention and the biological characteristics, functional identification, and antibacterial effect of the bacteriocin produced by it can be found in the patent "A bacteriocin Gr17 with broad-spectrum antibacterial activity and its application "(public number: CN109627299A). The Enterococcus faecalis Gr17 was isolated from traditional Chinese low-salt fermented whole fish products, and its complete genome sequence was determined; The molecular weight of coccus bacteriocin Gr17 is 4531.01Da; bacteriocin Gr17 is effective against Listeria monocytogenes (Listeriamonocytogenes), Staphylococcus aereus (Staphylococcus aereu), Bacillus subtilis (Bacillus subtilis), Escherichia coli (Escherichia coli), Sakazaki intestinalis Enterobacter sakazakii, Enterococcus faecalis, Pseudomon...
Embodiment 3
[0053] Example 3 The active ingredient of Monascus purple CGMCC No.12502 and its application in fermented bean curd
[0054] For the biological characteristics and active ingredients produced by the Monascus purpureus CGMCC No.12502 used in the present invention, see the patent "A Blood-Lipid-Lowering Oatmeal Red Koji Health Tea and Its Preparation Method" (publication number: CN106173054A). The bacterium is the "M1-20" strain selected by the applicant's team through mutagenesis, and the preservation number is "CGMCC No. 12502". The content of Monacolin K in the fermentation broth of M1-20 strain detected by HPLC reached 421.7mg / L, which was 142.1% higher than that of the original strain M1, and its ability to produce Monacolin K only decreased by 4.3% after 10 subcultures, showing good genetic stability , indicating that the M1-20 strain has the characteristics of high-yielding functional component Monacolin K. The breeding process and characteristics of the M1-20 strain are...
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