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Method for synergistically fermenting low-salt fermented bean curds by multifunctional bacteria and application

A collaborative fermentation and multi-functional technology, applied in the direction of microorganism-based methods, applications, biochemical equipment and methods, etc., can solve the problems of high salt content and single flavor of the product, and achieve the reduction of salt content, increase of flavor components, and outstanding floral fragrance Effect

Pending Publication Date: 2020-11-24
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problems of high salt content in traditional fermented bean curd, potential safety risks after salt reduction, and single flavor of the product, the present invention provides a method for synergistically fermenting flower-flavored low-salt fermented bean curd with multifunctional bacteria and a new product thereof, which can make fermented bean curd products The salinity is controlled at 3% to 5%, eliminating biogenic amines and "white spots", fully fermenting, realizing low-salinity products, improving the flavor components, functional components and nutritional quality of fermented bean curd, and eliminating potential safety risks

Method used

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  • Method for synergistically fermenting low-salt fermented bean curds by multifunctional bacteria and application
  • Method for synergistically fermenting low-salt fermented bean curds by multifunctional bacteria and application
  • Method for synergistically fermenting low-salt fermented bean curds by multifunctional bacteria and application

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Embodiment 1

[0027] The preparation method of embodiment 1 low-salt fermented bean curd, comprises the following steps.

[0028] (1) Domestication and improvement of Mucor radiata and Aspergillus oryzae

[0029] Add L-tyrosine to the PDA agar medium (gradually increase the L-tyrosine concentration in the range of 0-0.04%), inoculate Mucor actinosa and Aspergillus oryzae, and carry out domestication by repeated passage for 15 generations. The activity of phthaloyl tyrosine hydrolase in Mucorra jazhi and Aspergillus oryzae decreased significantly, and their tyrosine-producing ability decreased by 34.3% and 32.5% respectively compared with unaccustomed strains, and the white spot rate of fermented bean curd decreased.

[0030] (2) Preparation of starter

[0031] The preparation process of the mold spore suspension of the described Monascus purple, domesticated radiomucor yazhi, and domesticated Aspergillus oryzae is as follows: inoculate the three kinds of molds on the PDA plate medium respe...

Embodiment 2

[0048] Example 2 Antibacterial activity of bacteriocin produced by Enterococcus faecalis and its application in fermented bean curd

[0049] Enterococcus faecalis (E.Faecium) CGMCC No.16677 used in the present invention and the biological characteristics, functional identification, and antibacterial effect of the bacteriocin produced by it can be found in the patent "A bacteriocin Gr17 with broad-spectrum antibacterial activity and its application "(public number: CN109627299A). The Enterococcus faecalis Gr17 was isolated from traditional Chinese low-salt fermented whole fish products, and its complete genome sequence was determined; The molecular weight of coccus bacteriocin Gr17 is 4531.01Da; bacteriocin Gr17 is effective against Listeria monocytogenes (Listeriamonocytogenes), Staphylococcus aereus (Staphylococcus aereu), Bacillus subtilis (Bacillus subtilis), Escherichia coli (Escherichia coli), Sakazaki intestinalis Enterobacter sakazakii, Enterococcus faecalis, Pseudomon...

Embodiment 3

[0053] Example 3 The active ingredient of Monascus purple CGMCC No.12502 and its application in fermented bean curd

[0054] For the biological characteristics and active ingredients produced by the Monascus purpureus CGMCC No.12502 used in the present invention, see the patent "A Blood-Lipid-Lowering Oatmeal Red Koji Health Tea and Its Preparation Method" (publication number: CN106173054A). The bacterium is the "M1-20" strain selected by the applicant's team through mutagenesis, and the preservation number is "CGMCC No. 12502". The content of Monacolin K in the fermentation broth of M1-20 strain detected by HPLC reached 421.7mg / L, which was 142.1% higher than that of the original strain M1, and its ability to produce Monacolin K only decreased by 4.3% after 10 subcultures, showing good genetic stability , indicating that the M1-20 strain has the characteristics of high-yielding functional component Monacolin K. The breeding process and characteristics of the M1-20 strain are...

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Abstract

Fermented bean curds are a traditional soybean fermented food and seasoning in China, and the salt content of traditional fermented bean curds is generally 8%-16%. In recent years, low-salinity dietsare beneficial to health and becomes a consensus of the public, and low-salinity development of traditional fermented foods becomes a social demand. Aiming at the problems of high salt content, potential safety risks after salt reduction and the like of the traditional fermented bean curds, the invention provides a method for synergistically fermenting flowery low-salt fermented bean curds by multifunctional bacteria, so that the fermented bean curd products are low in salt content, palatable in saltiness, mellow and rich in fragrance and outstanding in flowery flavor; the fermented bean curdscontain functional components such as Monacolin K and gamma-aminobutyric acid (GABA), so that the flavor and quality of the fermented bean curds are effectively improved, and the upgrading and updating of the traditional fermented bean curd industry are guided.

Description

technical field [0001] The invention relates to a method and application of multifunctional bacteria cooperatively fermenting flower-flavored low-salt fermented bean curd, which belongs to the field of food science and technology. Background technique [0002] Fermented bean curd is a kind of traditional soybean fermented food in my country. Sufu contains various nutrients such as free amino acids, calcium, phosphorus, riboflavin and B vitamins, and contains functional ingredients such as antioxidant peptides, ACE inhibitory peptides, and soybean isoflavones. [0003] At present, the production of fermented bean curd is mainly carried out by pre-fermentation inoculation with a single purebred microorganism to make the dough, and post-fermentation by natural fermentation. According to the microorganisms inoculated in the pre-fermentation, fermented bean curd can be divided into mucor type, rhizopus type, and bacterial type. The commonly used mucormycetes are mainly Actinomrc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23C20/02C12N1/14C12N1/20C12R1/645C12R1/01C12R1/785C12R1/69
CPCA23C20/025C12N1/14C12N1/20
Inventor 王成涛刘国荣张婵赵磊袁栋栋赵亮孙宝国王欣赵丹
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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