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Degradation and deodorization bacteria agent for human feces and method thereof

A technology for deodorizing bacteria and feces, which is applied in the field of environmental microorganisms, can solve the problems of reducing the concentration of odorous VOCs, low activity of microbial agents, and complex bacteria, so as to achieve high total effective viable bacteria content and maintain optimal temperature and humidity. , the effect of high enzymatic activity

Active Publication Date: 2021-12-28
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to provide a low-cost degradation and deodorizing bacterial agent and its method that can quickly degrade human feces and effectively reduce the concentration of malodorous VOCs in feces, so as to solve the problem of low activity, high cost, and bacterial contamination of existing microbial agents for treating human feces. overly complex and other issues, while meeting the needs of waterless toilets

Method used

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  • Degradation and deodorization bacteria agent for human feces and method thereof
  • Degradation and deodorization bacteria agent for human feces and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] In this example, the strains that can be used for the degradation of human feces were subjected to directional isolation and screening, as follows:

[0043] (1) Sample environmental genome analysis

[0044] The litter samples (buckwheat husks, sawdust, wood blocks and other mixtures) provided by Hangzhou Ivy Industrial Co., Ltd. were subjected to PCR amplification after DNA extraction and detection. According to the characteristics of the amplified region, a small fragment library was constructed and sequenced based on IlluminaNovaSeq The platform performs paired-end sequencing on the library. The original data is spliced ​​and filtered to obtain valid data. OTUs (Operational Taxonomic Units) clustering and species classification analysis were performed based on valid data. According to the OTUs clustering results, species annotations were made on the representative sequences of each OTU, and species classification tree statistics were performed according to the speci...

Embodiment 2

[0048] In the present embodiment, prepared a kind of degradation deodorizing bacteria agent, specifically as follows:

[0049] S1: Inoculate Bacillus sucrose and Bacillus subtilis into LB liquid medium (10g sodium chloride, 10g tryptone, 5g yeast extract, 1L sterile water, pH 7.0) and CM medium (5g sodium chloride, 5g of beef extract powder, 10g of tryptone, 1L of sterile water, pH 7.0), and cultivated at a temperature of 32°C and 180rpm for 72h to achieve expanded cultivation to obtain Bacillus subtilis bacterium liquid and Saccharomyces sucrose bacterium liquid.

[0050] S2: 3kg of corncobs (particle size 1-2cm) and 4.5kg of bamboo chips (passed through a 4-mesh sieve) were sterilized by high-pressure steam at 121°C for 30 minutes and mixed evenly, and 375g of cornstarch, 375g of soybean meal and 2L of nutrient solution were added (37.5g ammonium sulfate, 37.5g potassium dihydrogen phosphate, 7.5g sodium chloride, 7.5g magnesium sulfate heptahydrate, 0.75g manganese sulfate,...

Embodiment 3

[0057] In the present embodiment, the degradation and deodorization bacterial agent prepared in Example 2 is used for the research on the degradation and deodorization effect of human feces, as follows:

[0058] (1) Bacteria application

[0059] Add 5 kg of bacterial agent to the dry toilet machine (provided by Hangzhou Ivy Industry Co., Ltd.), control the temperature and humidity of the bacterial agent to be 40°C and 55% respectively, and simulate a family of 3 daily excretion situations. Stir for 1 minute, vent for 5 minutes, then stir for 1 minute and vent for 10 minutes every 60 minutes. The reactor was operated continuously for 35 days, during which the concentration of malodorous VOCs was detected every 7 days, and the contents of cellulose, protein, starch, and fat in the reactor were measured on the 30th day.

[0060] (2) Sample detection method

[0061]Determination of the starch content of the material (perchloric acid hydrolysis-anthrone sulfuric acid colorimetric...

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Abstract

The invention discloses a degrading and deodorizing bacterial agent for human feces and a method thereof. The degrading and deodorizing bacterial agent is composed of Bacillus subtilis and Bacillus sucrose; the Bacillus subtilis was preserved on October 9, 2020 In the General Microbiology Center of the China Committee for the Collection of Microbial Cultures, the preservation number is CGMCC No.20844; the said sucrose was preserved in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures on October 09, 2020, and the preservation number is CGMCC No. 20843. The invention is applied to dry toilet machines to realize efficient and rapid degradation of human feces, and at the same time effectively remove malodorous VOCs components in the feces. The bacterial agent of the present invention is constructed by compounding two strains of synergistic bacteria, the cultivation process is simple and easy, and the preparation is convenient; the solid fermentation substrate adopts raw materials such as corn cobs and bamboo chips with wide sources and cheap prices, and has low cost and wide application range. At the same time, the dried solid bacterial agent is also suitable for long-term storage.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a degrading and deodorizing bacterial agent for human feces and a method thereof. Background technique [0002] In recent years, with the rapid growth of population and GDP, people's living standards are improving day by day, and people's requirements for living environment are also increasing. The traditional dry toilets used in rural areas in northern my country have caused great harm to the environment and human health due to their heavy odor, overflowing feces, breeding of mosquitoes and flies, and simple and easy to collapse, which have seriously affected the people's sense of well-being At the same time, the use of traditional flush toilets consumes a lot of water resources and produces a large amount of toilet sewage that is difficult to treat, which is contrary to the concept of sustainable development. Therefore, it is imminent to promote ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F11/02C12R1/125C12R1/01
CPCC12N1/20C02F11/02C02F2303/02
Inventor 吴伟祥赵昶勋牟文建李馨予韩文婷王蓉悦
Owner ZHEJIANG UNIV