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Flora for degrading corn straw to produce polysaccharide and microbial ratio of flora

A technology for corn stalks and stalks, which is applied to microorganisms, isolated microorganisms, microorganism-based methods, etc., can solve problems such as poor degradation effect, and achieve the effects of high degradation efficiency, high polysaccharide yield, and high cellulose-degrading enzyme activity.

Pending Publication Date: 2021-04-16
TIANJIN UNIVERSITY OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using a single bacterial strain to degrade straw is less effective than mixed bacterial flora. Therefore, it is of great significance to construct a group of efficient and stable straw-degrading flora to promote the transformation and utilization of straw resources.

Method used

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  • Flora for degrading corn straw to produce polysaccharide and microbial ratio of flora
  • Flora for degrading corn straw to produce polysaccharide and microbial ratio of flora
  • Flora for degrading corn straw to produce polysaccharide and microbial ratio of flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Screening of degrading straw-producing polysaccharide-degrading bacteria and the construction of bacterial colonies

[0040] (1) Enrichment

[0041] Add 50g of straw powder to a 250ml Erlenmeyer flask, sterilize at 121°C for 40min, add 2g of soil sample, and incubate at 30°C for 20 days.

[0042] (2) Primary screening of cellulose degrading bacteria

[0043] Take 1g of the enriched material in (1) in a Erlenmeyer flask filled with 99ml of sterile water, incubate on a constant temperature shaker for 45 minutes with shaking at a speed of 120 times per minute, and apply the supernatant to sodium carboxymethylcellulose On a solid medium, culture at a constant temperature of 30°C. After colonies grow out, pure colonies are isolated by streaking.

[0044] Place the single strains screened for the first time in the cellulose Congo red medium, culture at 30°C for 3 days, measure the ratio of the transparent circle to the colony diameter, select the strain with a la...

Embodiment 2

[0069] Example 2: Degradation efficiency of the constructed flora to corn stalks

[0070] (1) Quantitative determination of corn stalk cellulose

[0071] Dry corn stalks (hereinafter referred to as: stalks) to constant weight, add 0.5 g of stalks to the Erlenmeyer flask, and then add 50 mL of enzyme-producing medium, place the high-efficiency flora constructed in Example 1 in sodium carboxymethyl cellulose Activated in the medium for 2 days, transferred to the enzyme-producing medium at 30°C, 120r / min, and cultivated for 8 days. On the 8th day, the straw was taken out and the weight loss rate and the content of each component of the straw were measured.

[0072] (2) Determination and change trend of each component content of straw

[0073] The content of each component (cellulose, hemicellulose, lignin) in straw was determined by differential gravimetric method.

[0074] The result is as follows:

[0075] Such as image 3 As shown in Table 3, the contents of the three majo...

Embodiment 3

[0082] Example 3: Determination of the content of polysaccharides produced by degrading corn stalks by the constructed flora

[0083] The mensuration method of polysaccharide content adopts 3,5-dinitrosalicylic acid method (hereinafter referred to as: DNS method) to draw glucose standard curve, and contrast standard curve asks sugar content, concrete steps are:

[0084] (1) Activation of bacteria

[0085] Use an inoculation loop to inoculate the strain into 50ml of activation medium, activate at 30°C, 120r / min for 48h.

[0086] The activation medium formula is: 15g CMC-Na, 1g yeast extract, 1g NH 4 NO 3 , 1g KH 2 PO 4 , 0.5gMgSO 4 , 0.5g FeSO 4 ·7H 2 O, 20g agar, 1000mL distilled water, natural pH.

[0087] (2) Treatment of fermentation broth

[0088] Add 50 mL of straw fermentation medium into the Erlenmeyer flask, and sterilize at 121 °C for 20 min. Then inoculate it with 4% (2ml) of the flora suspension described in (1), and cultivate it at 30°C for 2 days at 120r...

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Abstract

The invention discloses a flora for degrading corn straw (hereinafter referred to as straw) to produce polysaccharide, and belongs to the technical field of solid waste disposal. The mixed flora comprises streptomyces griseorubens, streptomyces carpaticus and streptomyces rochei, and the inoculation amount is 1:1:1. The degrading strains are respectively named ye-9, er-72 and se-93, and are preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation numbers are respectively 18289, 18290 and 18291, and the preservation date is July 24th, 2019. According to the invention, a single strain is primarily screened from a sample, and the primarily screened single strain is secondarily screened by taking the activities of four enzymes, namely cellulose whole enzyme, incision enzyme, excision enzyme and beta-glucosidase, as indexes. The strains which are obtained by secondary screening and have a good degradation effect are obtained through screening, the enzyme activity is determined, and efficient mixed flora is picked out. The straw degradation effect of the mixed flora is better than that of a single strain, and the polysaccharide yield is as high as 2159.23 mg / L under the optimized fermentation condition. A basis is provided for microbial degradation of the straws, and resource utilization of the straws is realized.

Description

technical field [0001] The invention belongs to the technical field of solid waste disposal, and relates to a group of bacterial flora and its microbial ratio, in particular to a group of bacterial flora and its microbial ratio for degrading corn stalks (hereinafter referred to as: straw) to produce polysaccharides. Background technique [0002] my country's corn stalks are extremely rich in resources, which can provide a large amount of glycogen substances for human beings, but the lack of full and effective use of them results in waste of resources and even environmental pollution. According to the sustainable development strategy, the efficient transformation and utilization of corn stalk resources is an important content in the field of agricultural solid waste treatment and disposal. [0003] Corn stalks have complex components and are not easy to degrade in a short period of time. The use of physical and chemical methods consumes a lot of energy and pollutes the enviro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C12P19/14C12P19/04C12R1/465
Inventor 解玉红冯炘孙景红
Owner TIANJIN UNIVERSITY OF TECHNOLOGY
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