Immobilized enzyme honey and preparation method thereof

A technology for immobilizing enzymes and honey, which is applied in chemical instruments and methods, semipermeable membrane separation, food ingredients as taste improvers, etc. It can solve the problems of poor filtration effect and cumbersome process, so as to reduce the content, simplify the purification process, The effect of high yield

Pending Publication Date: 2021-04-30
江西巨荣生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the process of honey processing, since raw honey contains impurities, filtration is particularly important. Existing filtration devices generally include several filtration steps to obtain honey with the same fineness.
In fact, due to the different sources of honey and the different taste requirements of different groups of people on the fineness of honey, this requires that in the process of honey processing, one filter honey, two filter honey, and multiple filter honey can be separated. Cumbersome and poor filtering

Method used

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  • Immobilized enzyme honey and preparation method thereof
  • Immobilized enzyme honey and preparation method thereof
  • Immobilized enzyme honey and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A solidified enzyme honey is composed of the following components: parts by weight: 80 parts of acacia honey, 3 parts of pineapple juice, 5 parts of grapefruit juice, 3 parts of passion fruit juice, 3 parts of mangosteen juice, 4 parts of dragon fruit, 2 parts of grape juice 12 parts of water, 3-5 parts of compound solidified enzyme.

[0034] The preparation method of the solidified enzyme honey and the composite solidified enzyme is: based on the total weight of the substrate: glucoamylase 100U / g, cellulase 50U / g, bromelain 30U / g, pectin esterase 20U / g mixed , adjust the pH to 7.1, raise the temperature to 32°C, and keep it for 35 minutes; then mix in polyphenol oxidase 30U / g, glucosidase 100U / g, lipoxygenase 50U / g, adjust the pH to 7.5, cool down to 28.5°C, Keep it for 60 minutes; prepare immobilized enzyme by cross-linking method, add 0.1 g / g polyethylene glycol colloid particles based on the total weight of the solution to the above mixed enzyme solution, the partic...

Embodiment 2

[0046] A solidified enzyme honey, consisting of the following components: parts by weight: 70 parts of acacia honey, 2 parts of pineapple juice, 3 parts of grapefruit juice, 2 parts of passion fruit juice, 2 parts of mangosteen juice, 3 parts of dragon fruit, 1 part of grape juice 10 parts, 10 parts of water, compound solidified enzyme.

[0047] The preparation method of the composite immobilized enzyme is: based on the total weight of the substrate: 100 U / g of glucoamylase, 50 U / g of cellulase, 30 U / g of bromelain, and 20 U / g of pectin esterase are mixed, and the pH is adjusted to 7.1 , heated to 32°C, and kept for 35min; then mixed with polyphenol oxidase 30U / g, glucosidase 100U / g, lipoxygenase 50U / g, adjusted to pH 7.5, cooled to 28.5°C, and kept for 60min; Combined method to prepare immobilized enzyme, adding 0.1g / g polyethylene glycol colloid particles based on the total weight of the solution to the above mixed enzyme solution, the particle diameter is 12 μm; the mass fr...

Embodiment 3

[0050] A solidified enzyme honey, consisting of the following components: parts by weight: 90 parts of acacia honey, 5 parts of pineapple juice, 8 parts of grapefruit juice, 5 parts of passion fruit juice, 5 parts of mangosteen juice, 6 parts of dragon fruit, 3 parts of grape juice parts, 15 parts of water, compound solidified enzyme.

[0051] The preparation method of the composite immobilized enzyme is: based on the total weight of the substrate: 100 U / g of glucoamylase, 50 U / g of cellulase, 30 U / g of bromelain, and 20 U / g of pectin esterase are mixed, and the pH is adjusted to 7.1 , heated to 32°C, and kept for 35min; then mixed with polyphenol oxidase 30U / g, glucosidase 100U / g, lipoxygenase 50U / g, adjusted to pH 7.5, cooled to 28.5°C, and kept for 60min; Combined method to prepare immobilized enzyme, adding 0.1g / g polyethylene glycol colloid particles based on the total weight of the solution to the above mixed enzyme solution, the particle diameter is 15 μm; the mass frac...

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Abstract

The invention provides immobilized enzyme honey, which is prepared from the following components in parts by weight: 70-90 parts of acacia honey, 2-5 parts of pineapple juice, 3-8 parts of grapefruit juice, 2-5 parts of passion fruit juice, 2-5 parts of mangosteen juice, 3-6 parts of pitaya, 1-3 parts of grape juice, 10-15 parts of water and 3-5 parts of compound immobilized enzyme. The formula used in the present invention uses honey as a substrate and fruit juice as an auxiliary material. The problems that pure honey is single in taste and heavy in sweetness are solved, the added compound fruits can effectively improve the too sweet and greasy taste feeling of the honey and bring the fresh feeling of the fruits, impurities and cellulose generated through enzymolysis can be effectively filtered out through a novel filter and a filtering membrane, the honey product is soft in taste, the content of enzymatic hydrolysate in the honey can be effectively reduced, and more oxidation resistance of total phenols, total flavonoids, proline and the like is reserved.

Description

technical field [0001] The invention relates to the field of honey food, in particular to enzyme-cured honey and a preparation method thereof. Background technique [0002] Honey is the honey brewed by bees. Bees collect nectar or secretions with a water content of about 75% from the flowers of plants, store them in their second stomach, and have multiple functions in their bodies. After repeated brewing, vitamins, Minerals and amino acids are rich to a certain value. At the same time, the polysaccharides in the nectar are converted into simple sugars glucose and fructose that can be directly absorbed by the human body in the body of the bee. The water content is less than 23% and then stored in the nest. Seal with beeswax. Therefore, honey is rich in nutrition, including a variety of micronutrients: vitamin A, vitamin B1, vitamin B2, vitamin B6, vitamin C, vitamin D, vitamin K, niacin, pantothenic acid, folic acid, biotin, choline, etc.; Acids: citric acid, malic acid, su...

Claims

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Application Information

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IPC IPC(8): A23L21/25A23L19/00A23L29/00A23L5/00B01D61/14
CPCA23L21/25A23L19/09A23L29/06A23L5/00B01D61/145A23V2002/00A23V2200/14
Inventor 范斌斌
Owner 江西巨荣生物科技有限公司
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